FastQCFastQC Report
Thu 26 May 2016
SRR1545787_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545787_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences434253
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10940.25192687212293297No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.2300502241780713No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5090.11721277688352182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAATA207.028432E-445.0000041
GCGAATA207.028432E-445.0000041
GTTACGG207.028432E-445.0000042
CCGTAAG207.028432E-445.0000042
AACGCGT207.028432E-445.00000414
CGATGTA207.028432E-445.00000410
CGGTCTA800.045.00000431
TCGACAA406.7975634E-945.00000419
CACCACT207.028432E-445.00000439
TTCTCGT207.028432E-445.00000431
CAACCCG253.8867838E-545.023
AGGATCG502.1827873E-1145.07
TACCGGG502.1827873E-1145.03
CGAAGGA850.045.04
ACGGTCT850.045.030
AGGCGAT3450.044.3478247
ATAGGCG1250.043.1999975
TCACGAC1000.042.7525
CGTTTTA5750.042.6521761
CGACGGT900.042.528