##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545787_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	434253
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998811752595838	31.0	31.0	33.0	30.0	33.0
2	31.157539498863567	31.0	31.0	33.0	30.0	34.0
3	31.11159623537431	31.0	31.0	34.0	28.0	34.0
4	35.08856588210099	35.0	35.0	37.0	32.0	37.0
5	32.81361786792492	35.0	35.0	37.0	28.0	37.0
6	33.989142274204205	35.0	35.0	37.0	28.0	37.0
7	15.296115398166506	0.0	0.0	35.0	0.0	37.0
8	24.90032308354807	17.0	17.0	35.0	17.0	37.0
9	32.724699656651765	32.0	32.0	37.0	27.0	39.0
10	35.48075430682114	37.0	34.0	37.0	32.0	39.0
11	36.422497944746496	37.0	35.0	39.0	32.0	39.0
12	36.31314924709789	37.0	35.0	39.0	32.0	39.0
13	36.00858255440953	37.0	35.0	39.0	31.0	39.0
14	36.957748133000806	39.0	35.0	40.0	31.0	41.0
15	37.28711833884855	39.0	36.0	41.0	32.0	41.0
16	37.327937861108616	39.0	35.0	41.0	32.0	41.0
17	37.285626121178204	39.0	35.0	41.0	32.0	41.0
18	37.2384853990646	39.0	35.0	40.0	32.0	41.0
19	37.199047559832636	39.0	35.0	40.0	32.0	41.0
20	37.30168818637983	39.0	35.0	40.0	32.0	41.0
21	37.186260083407596	39.0	35.0	40.0	32.0	41.0
22	37.26890545373319	39.0	35.0	41.0	32.0	41.0
23	37.261260140977726	39.0	35.0	41.0	32.0	41.0
24	37.102249149689236	39.0	35.0	40.0	32.0	41.0
25	36.835998830175036	38.0	35.0	40.0	31.0	41.0
26	36.725464187927315	38.0	35.0	40.0	31.0	41.0
27	36.63349245716207	38.0	35.0	40.0	31.0	41.0
28	36.54189378081441	38.0	35.0	40.0	31.0	41.0
29	36.502911896981715	38.0	35.0	40.0	31.0	41.0
30	36.2078534863317	38.0	35.0	40.0	30.0	41.0
31	36.062207975534996	38.0	35.0	40.0	30.0	41.0
32	35.973764142101494	38.0	34.0	40.0	30.0	41.0
33	35.80139457873636	38.0	34.0	40.0	29.0	41.0
34	35.611958927169184	38.0	34.0	40.0	28.0	41.0
35	35.43799582271164	38.0	34.0	40.0	27.0	41.0
36	35.22684241674784	38.0	34.0	40.0	26.0	41.0
37	35.08942482838346	38.0	34.0	40.0	25.0	41.0
38	34.92439200189751	38.0	34.0	40.0	25.0	41.0
39	34.81688324548132	38.0	34.0	40.0	24.0	41.0
40	34.63819478506769	38.0	33.0	40.0	24.0	41.0
41	34.46991270066067	37.0	33.0	40.0	23.0	41.0
42	34.434205405604565	37.0	33.0	40.0	23.0	41.0
43	34.33907422631508	37.0	33.0	40.0	23.0	41.0
44	34.02172466281177	37.0	33.0	40.0	23.0	41.0
45	33.98291548935759	37.0	33.0	40.0	23.0	41.0
46	34.007417335055834	36.0	33.0	40.0	23.0	41.0
47	33.91074788199506	36.0	33.0	40.0	23.0	41.0
48	33.83926420773144	36.0	33.0	40.0	23.0	41.0
49	33.742516459299075	36.0	33.0	40.0	22.0	41.0
50	33.614376872468355	36.0	33.0	40.0	22.0	41.0
51	33.45269693013059	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	9.0
15	26.0
16	66.0
17	128.0
18	282.0
19	494.0
20	878.0
21	1473.0
22	2280.0
23	3359.0
24	4739.0
25	6297.0
26	7806.0
27	8410.0
28	9343.0
29	10828.0
30	13177.0
31	16718.0
32	22350.0
33	31902.0
34	40393.0
35	44184.0
36	52801.0
37	76261.0
38	66354.0
39	13689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.61185069533199	23.945948559940863	27.626061305275957	13.816139439451195
2	34.15566501555545	23.69402168781793	29.994496295938085	12.155817000688538
3	31.68936081040315	23.206517859404542	32.15222462481549	12.95189670537682
4	27.874994530838016	27.10286399863674	29.64677273386712	15.375368736658125
5	21.26663488795702	36.14367661248166	28.423752973497017	14.165935526064299
6	20.3535726868899	41.908288486205045	27.828017307882728	9.91012151902232
7	38.536751617144844	56.706343997623506	3.979707681927356	0.777196703304295
8	88.62137970261576	2.845345915860109	6.901276444837457	1.6319979366866781
9	84.82405418039714	3.8634160270625646	8.314507902075519	2.9980218904647753
10	48.77387145281668	24.486186623926606	14.160869354961278	12.579072568295441
11	41.66557283426942	22.231970763587125	20.898416360969296	15.204040041174155
12	37.68056870073436	20.746891788888043	26.378631811409477	15.193907698968111
13	24.560567226939135	31.74278588748955	27.409367350369486	16.28727953520183
14	18.85352547938644	35.18686111552482	29.612460938669393	16.347152466419345
15	17.30995525649794	23.00064708821816	42.4697123566216	17.219685298662302
16	20.608262925069027	17.96717581686252	40.60374942717724	20.82081183089121
17	20.342288942160447	18.448692352154158	29.104462145339237	32.10455656034616
18	23.35689102896238	22.30911473265585	33.82705473537316	20.506939503008613
19	29.182987797436056	24.27824332819808	25.43655426675233	21.102214607613533
20	29.947749353487485	24.313706525919223	24.67570747928051	21.06283664131278
21	25.561366300290384	25.996596454140793	29.016955553559793	19.425081692009037
22	23.955159780128174	21.869048688207105	27.498946466691077	26.67684506497364
23	21.602383863784475	28.534748176754103	25.840466271965884	24.022401687495538
24	22.082058155038652	25.357568053646144	33.50834651689223	19.052027274422976
25	22.604794900668505	23.277213974342146	32.00162117475296	22.116369950236383
26	19.746322996041478	29.284541500001147	26.712768823704153	24.256366680253215
27	20.223233921239462	28.177122552981785	29.86553921331574	21.734104312463014
28	18.57350438569221	26.54673657982789	34.7104107513362	20.169348283143698
29	20.287021621036587	25.53350235922377	32.943468438905434	21.236007580834215
30	22.29069229228123	26.696418907871678	30.86956221373255	20.143326586114547
31	29.805666282098226	24.007894015700526	25.801318586169813	20.38512111603144
32	29.938768413804855	24.832528502969467	25.711739469848222	19.516963613377456
33	28.49513992994867	24.41134545990471	25.623772317059412	21.46974229308721
34	21.224954116609442	25.788192597402897	28.345918162914245	24.640935123073415
35	21.825295392317383	24.52164982164775	30.068186057436563	23.584868728598305
36	30.787121793056123	23.838177283749335	24.884111336018407	20.49058958717614
37	22.32085903839467	30.153389844169183	26.05831162939577	21.467439488040384
38	22.649008757567593	30.634676098956138	23.924302192500686	22.792012950975582
39	22.12673257294711	28.005103015983767	24.7599901439944	25.108174267074723
40	25.060736483110073	23.291261085127793	26.60545810852199	25.04254432324014
41	20.17878978383569	22.54331000591821	27.495492259120834	29.78240795112527
42	22.16449857571508	25.20304983500402	25.234828544650238	27.397623044630663
43	21.741243008108178	24.413648264951537	26.99670468597799	26.848404040962297
44	21.52155540664083	26.192795444130496	29.243091009158256	23.042558140070422
45	20.890126262800717	29.92426074200984	24.96079474407776	24.224818251111678
46	23.393045068197573	27.750643058309326	27.659451978454957	21.196859895038145
47	22.552290945600838	26.960320366238115	26.887321446253683	23.600067241907368
48	22.12535088991901	26.317146916659183	28.816611514485796	22.74089067893601
49	22.339281478769287	24.484804940898506	30.494665552109023	22.681248028223177
50	21.35045699166154	28.29686841541682	27.673268808735923	22.679405784185715
51	20.520526052784895	30.15937713729093	26.130619707866153	23.189477102058017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	158.0
1	351.0
2	544.0
3	2366.5
4	4189.0
5	3036.5
6	1884.0
7	1940.0
8	1996.0
9	2006.0
10	2016.0
11	1964.0
12	1912.0
13	1820.5
14	1729.0
15	1671.5
16	1614.0
17	1580.0
18	1546.0
19	1607.0
20	1668.0
21	1697.5
22	1727.0
23	1947.5
24	2168.0
25	2370.5
26	2904.0
27	3235.0
28	3859.0
29	4483.0
30	5161.5
31	5840.0
32	6873.5
33	7907.0
34	8920.0
35	9933.0
36	11296.5
37	12660.0
38	15746.0
39	18832.0
40	23188.0
41	27544.0
42	30251.5
43	32959.0
44	33378.5
45	33798.0
46	34884.5
47	35971.0
48	35180.0
49	34389.0
50	32616.0
51	30843.0
52	28641.5
53	26440.0
54	25044.0
55	23648.0
56	23011.5
57	22375.0
58	21191.5
59	20008.0
60	18178.0
61	16348.0
62	14566.5
63	12785.0
64	11017.0
65	9249.0
66	7835.0
67	6421.0
68	5581.0
69	4741.0
70	4045.0
71	3349.0
72	2828.0
73	2307.0
74	1782.0
75	905.5
76	554.0
77	451.5
78	349.0
79	262.5
80	176.0
81	129.0
82	82.0
83	56.0
84	30.0
85	18.0
86	6.0
87	5.0
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.35352868677625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.34587038305229	26.896511707421393
2	9.480578031929507	7.461883990935172
3	5.228513109310564	6.172813219093168
4	3.5050725391342485	5.517478908722053
5	2.6029961952087377	5.121854271985825
6	1.9811886723446397	4.678005915061856
7	1.6074554585999312	4.4281331151909225
8	1.2772471500934408	4.0211345889044345
9	0.9747713792076108	3.452462409122565
>10	4.9372174141368275	29.25388678409343
>50	0.03802800350338837	1.0181050388589576
>100	0.01930652485556634	1.3786551272472682
>500	0.001170092415488869	0.3471963814109687
>1k	5.850462077444345E-4	0.2518785419519892
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1094	0.25192687212293297	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	999	0.2300502241780713	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	509	0.11721277688352182	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011053464224772195	0.0
2	0.0	0.0	0.0	0.04375329588972327	0.0
3	0.0	0.0	0.0	0.05826096768473678	0.0
4	0.0	0.0	0.0	0.07990733512491566	0.0
5	0.0	0.0	0.0	0.1367866197815559	0.0
6	0.0	0.0	0.0	0.21485171086900953	0.0
7	0.0	0.0	0.0	0.2650528608898499	0.0
8	0.0	0.0	0.0	0.5869850064363401	0.0
9	0.0	0.0	0.0	0.7419637860878336	0.0
10	0.0	0.0	0.0	1.0090891715198282	0.0
11	0.0	0.0	0.0	1.243514725286872	0.0
12	0.0	0.0	0.0	1.4569847531277849	0.0
13	0.0	0.0	0.0	1.5470244304587417	0.0
14	0.0	0.0	0.0	1.5873235187782238	0.0
15	0.0	0.0	0.0	1.636603546780333	0.0
16	0.0	0.0	0.0	1.732169956223676	0.0
17	0.0	0.0	0.0	1.8480010500791013	0.0
18	0.0	0.0	0.0	2.0285409657503806	0.0
19	0.0	0.0	0.0	2.1165081185391927	0.0
20	0.0	0.0	0.0	2.2113836864684875	0.0
21	0.0	0.0	0.0	2.3113254255008027	0.0
22	0.0	0.0	0.0	2.4310712879358345	0.0
23	0.0	0.0	0.0	2.5584164070253976	0.0
24	0.0	0.0	0.0	2.663194036656051	0.0
25	0.0	0.0	0.0	2.7477069818746216	0.0
26	0.0	0.0	0.0	2.843273391317964	0.0
27	0.0	0.0	0.0	2.9445968133783764	0.0
28	0.0	0.0	0.0	3.042005466859181	0.0
29	0.0	0.0	0.0	3.152770389611586	0.0
30	0.0	0.0	0.0	3.304064681188155	0.0
31	0.0	0.0	0.0	3.4224288605950908	0.0
32	0.0	0.0	0.0	3.5377993934411505	0.0
33	0.0	0.0	0.0	3.6633022684932515	0.0
34	0.0	0.0	0.0	3.784890374965746	0.0
35	0.0	0.0	0.0	3.9557585094403493	0.0
36	0.0	0.0	0.0	4.084715592062691	0.0
37	0.0	0.0	0.0	4.225416980423854	0.0
38	0.0	0.0	0.0	4.402502688524892	0.0
39	0.0	0.0	0.0	4.739403066875761	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTAATA	20	7.028432E-4	45.000004	1
GCGAATA	20	7.028432E-4	45.000004	1
GTTACGG	20	7.028432E-4	45.000004	2
CCGTAAG	20	7.028432E-4	45.000004	2
AACGCGT	20	7.028432E-4	45.000004	14
CGATGTA	20	7.028432E-4	45.000004	10
CGGTCTA	80	0.0	45.000004	31
TCGACAA	40	6.7975634E-9	45.000004	19
CACCACT	20	7.028432E-4	45.000004	39
TTCTCGT	20	7.028432E-4	45.000004	31
CAACCCG	25	3.8867838E-5	45.0	23
AGGATCG	50	2.1827873E-11	45.0	7
TACCGGG	50	2.1827873E-11	45.0	3
CGAAGGA	85	0.0	45.0	4
ACGGTCT	85	0.0	45.0	30
AGGCGAT	345	0.0	44.347824	7
ATAGGCG	125	0.0	43.199997	5
TCACGAC	100	0.0	42.75	25
CGTTTTA	575	0.0	42.652176	1
CGACGGT	90	0.0	42.5	28
>>END_MODULE