FastQCFastQC Report
Thu 26 May 2016
SRR1545786_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545786_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1181931
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32680.2764966821244218No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25590.21651010084345026No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.1197193406383283No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13000.10998950023309313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAG207.856571E-444.0000046
GTCGAAA207.856571E-444.0000041
TAGGTCG207.856571E-444.0000046
CGTCTAG207.856571E-444.0000042
CGATCTA207.856571E-444.00000410
AGTACGT207.856571E-444.00000425
GTCGACC254.442916E-544.035
TACGATA254.442916E-544.01
AACGCAT454.802132E-1044.029
CGGTCAT254.442916E-544.033
CGACTCA254.442916E-544.035
GCGTATG454.802132E-1044.01
CGTTTTA21350.043.07261
CGGTCTA1650.042.66666431
AGGCGAT9350.042.1176457
AGTACGG1050.041.9047622
TAACGCC900.041.55555712
GTTTTAT24900.041.3493962
CCCGACG800.041.25000410
CGTTATT13500.040.5777781
Produced by FastQC (version 0.11.5)