##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545786_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1181931
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.514091770162555	31.0	31.0	33.0	30.0	34.0
2	31.69614216058298	31.0	31.0	34.0	30.0	34.0
3	31.76138031746354	31.0	31.0	34.0	30.0	34.0
4	35.65670246401863	37.0	35.0	37.0	33.0	37.0
5	27.082401595355396	35.0	25.0	37.0	0.0	37.0
6	31.317707209642524	35.0	26.0	37.0	17.0	37.0
7	15.023937945616115	0.0	0.0	32.0	0.0	37.0
8	25.11042607394171	17.0	17.0	35.0	17.0	37.0
9	33.22046887677876	32.0	32.0	37.0	28.0	39.0
10	36.0358481163452	37.0	35.0	39.0	32.0	39.0
11	36.997758752414484	39.0	37.0	39.0	34.0	39.0
12	36.916286991372594	39.0	35.0	39.0	33.0	39.0
13	36.36414900700633	39.0	35.0	39.0	32.0	39.0
14	37.64618323743095	40.0	36.0	41.0	33.0	41.0
15	37.95169684186302	40.0	36.0	41.0	33.0	41.0
16	38.04411678854349	40.0	36.0	41.0	33.0	41.0
17	38.084809519337426	40.0	36.0	41.0	34.0	41.0
18	38.06659779631806	40.0	36.0	41.0	34.0	41.0
19	38.00239438681277	40.0	36.0	41.0	34.0	41.0
20	38.01959589857614	40.0	36.0	41.0	34.0	41.0
21	37.86980373642793	40.0	36.0	41.0	33.0	41.0
22	37.813970527890376	40.0	35.0	41.0	33.0	41.0
23	37.73320862216153	40.0	35.0	41.0	33.0	41.0
24	37.59902566224255	39.0	35.0	41.0	33.0	41.0
25	37.416083510797165	39.0	35.0	41.0	33.0	41.0
26	37.18991463968709	39.0	35.0	40.0	33.0	41.0
27	36.957675194237225	39.0	35.0	40.0	32.0	41.0
28	37.01118762431987	39.0	35.0	40.0	32.0	41.0
29	36.94589616483534	39.0	35.0	40.0	32.0	41.0
30	36.74849547054777	38.0	35.0	40.0	32.0	41.0
31	36.551906160342696	38.0	35.0	40.0	31.0	41.0
32	36.35642266765149	38.0	35.0	40.0	31.0	41.0
33	36.17604919407309	38.0	35.0	40.0	30.0	41.0
34	35.949004637326546	38.0	35.0	40.0	30.0	41.0
35	35.816200776525875	38.0	35.0	40.0	30.0	41.0
36	35.60862097702827	38.0	35.0	40.0	29.0	41.0
37	35.47754902781973	38.0	34.0	40.0	29.0	41.0
38	35.288785893592774	38.0	34.0	40.0	28.0	41.0
39	35.251624671829404	38.0	34.0	40.0	28.0	41.0
40	35.013472021632396	37.0	34.0	40.0	27.0	41.0
41	34.820750111470126	37.0	34.0	40.0	26.0	40.0
42	34.83616217867202	37.0	34.0	40.0	27.0	41.0
43	34.71776778847496	37.0	34.0	40.0	26.0	41.0
44	34.52329535311283	37.0	34.0	40.0	26.0	40.0
45	34.25006620521841	36.0	33.0	39.0	25.0	40.0
46	34.237039218025416	36.0	33.0	39.0	25.0	40.0
47	34.083086914549156	36.0	33.0	39.0	24.0	40.0
48	34.00933895464287	36.0	33.0	39.0	24.0	40.0
49	33.91661865201945	36.0	33.0	39.0	24.0	40.0
50	33.96011019255777	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	11.0
15	30.0
16	59.0
17	177.0
18	446.0
19	908.0
20	1624.0
21	2742.0
22	4374.0
23	6568.0
24	9751.0
25	13684.0
26	17241.0
27	18978.0
28	20807.0
29	23968.0
30	29647.0
31	40246.0
32	55491.0
33	101871.0
34	102854.0
35	125012.0
36	160800.0
37	287490.0
38	126807.0
39	30338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.89349209048582	22.655721865320395	28.101471236476577	14.349314807717201
2	33.34873186336596	23.20930748072434	30.525893643537565	12.916067012372126
3	30.7066994604592	23.52074698099974	32.760880288274016	13.011673270267046
4	26.8598589934607	26.346377241987902	31.079563866249384	15.714199898302017
5	17.385278836074185	47.97995822091137	23.11090918166966	11.523853761344782
6	20.58842690478547	41.33117753912877	28.118138876127286	9.962256679958474
7	39.605103851240045	55.75646970931467	3.6194160234396087	1.019010416005672
8	88.00945232843542	3.001698068668983	7.090177006948799	1.8986725959468023
9	84.32404260485595	4.271907581745466	8.659727175275037	2.7443226381235455
10	52.504418616653595	22.022013129362037	13.865530221307335	11.608038032677035
11	46.50889095894769	20.679464368055324	19.257130915425687	13.554513757571296
12	40.407942595633756	21.022547001474706	24.482055213036972	14.08745518985457
13	22.800146539857234	37.59804929390971	25.019142403405954	14.582661762827104
14	17.18425187257124	39.6013811296937	28.752947507087978	14.461419490647085
15	15.447348449274958	22.60411140751871	46.77024293296309	15.178297210243239
16	17.860433477081152	17.1709685252354	45.03976966506505	19.9288283326184
17	18.601678101344326	17.476739335883398	28.86615208502019	35.05543047775209
18	23.212691773039204	22.03123532592004	34.27763549648837	20.47843740455238
19	30.470898893421023	23.053122390393348	25.224145910378866	21.25183280580677
20	31.1876920057093	23.41422638038938	24.673436943442553	20.724644670458765
21	24.29642677956666	26.237741458680752	29.346467771807323	20.119363989945267
22	24.280605212994665	23.020210147631293	26.161256452364817	26.537928187009225
23	20.643337047594148	29.402223987694715	25.472129929750555	24.482309034960586
24	21.05545924423676	24.593990681351112	36.34036166239823	18.0101884120139
25	20.27918719451474	23.160658278698165	34.64753864650305	21.912615880284044
26	18.74314152010566	30.755856306332603	27.43671161852934	23.0642905550324
27	19.064057038862675	30.523016995069934	29.856396016349517	20.556529949717877
28	17.253799079641706	26.812648115668342	37.539416429554684	18.39413637513527
29	18.07965101177649	24.64280909799303	35.946091607716525	21.331448282513954
30	20.98489674947184	27.54170928759801	31.564702169585196	19.90869179334496
31	30.319197990407226	24.931066195911605	24.47723259648829	20.272503217192885
32	30.323005319261448	24.73959985819815	26.58395456249138	18.353440260049023
33	28.75066310977544	24.52918148352146	25.54556907298311	21.17458633371999
34	20.62963066371895	26.83549208879368	28.56698064438618	23.967896603101195
35	22.388447379753977	24.66861432689387	30.173758028175925	22.769180265176225
36	31.607513467368232	23.301360231688655	25.444547947384404	19.64657835355871
37	21.89027955100594	30.46506098917788	27.043456851542096	20.60120260827409
38	22.08631468334446	31.856089737895022	23.19450120184681	22.86309437691371
39	21.235334380771807	28.832816805718775	26.518383898890885	23.413464914618533
40	24.181107018937652	24.734608027033726	25.260527052763653	25.823757901264965
41	19.352314136781253	23.11996216361192	27.79341602851605	29.734307671090782
42	23.356947232960298	25.296315943993346	24.541026506623485	26.80571031642287
43	23.158712310617116	25.772147443463282	25.813943453551857	25.255196792367745
44	21.74627791300846	28.438546751037073	27.47554637284241	22.33962896311206
45	19.447243536213197	33.80484986010182	23.58310256690111	23.164804036783874
46	23.580564347664964	29.483277788635714	26.845560358430397	20.09059750526892
47	23.23037470038437	26.470665377251297	26.680406893465015	23.61855302889932
48	23.647319513575667	25.315944839419558	28.89288799430762	22.143847652697154
49	22.853956787663577	23.52599263408778	30.758732954800237	22.861317623448407
50	21.062143221558618	30.36708572666256	26.83261544032604	21.738155611452783
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	574.0
1	1103.5
2	1633.0
3	6089.5
4	10546.0
5	7880.0
6	5214.0
7	5386.5
8	5559.0
9	5933.5
10	6308.0
11	6294.5
12	6281.0
13	6038.5
14	5796.0
15	5391.0
16	4986.0
17	4867.0
18	4748.0
19	4620.5
20	4493.0
21	4664.5
22	4836.0
23	5203.0
24	5570.0
25	6338.5
26	7107.0
27	8373.5
28	9640.0
29	11208.0
30	12776.0
31	15205.0
32	17634.0
33	19443.0
34	21252.0
35	23715.0
36	26178.0
37	33781.0
38	41384.0
39	55447.0
40	69510.0
41	85201.0
42	100892.0
43	105346.0
44	109800.0
45	107715.0
46	105630.0
47	103516.5
48	101403.0
49	96955.5
50	92508.0
51	84166.5
52	75825.0
53	70063.5
54	64302.0
55	60503.5
56	56705.0
57	53186.0
58	49667.0
59	45695.0
60	41723.0
61	38051.5
62	34380.0
63	29286.0
64	24192.0
65	20867.5
66	17543.0
67	15194.0
68	12845.0
69	10904.5
70	8964.0
71	7454.5
72	5945.0
73	4794.0
74	3643.0
75	2875.5
76	2108.0
77	1511.5
78	915.0
79	710.5
80	506.0
81	366.0
82	226.0
83	171.0
84	116.0
85	80.5
86	45.0
87	29.5
88	14.0
89	8.5
90	3.0
91	2.0
92	1.0
93	3.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1181931.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.763092510291372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.58988997357369	16.29607288270039
2	6.9150887743014104	3.1481761097260073
3	3.1086104722293557	2.1228476327345214
4	2.0921693592261867	1.9049697868505089
5	1.5865175446995956	1.8057022819598607
6	1.263815071643052	1.7261003635026788
7	1.053020152194194	1.6778996597718279
8	0.9253345199666382	1.6850780224773316
9	0.8037436891060379	1.6466122754715249
>10	9.458572974787787	47.0952242246404
>50	1.0932706062760134	16.310850749517574
>100	0.10796746450518341	3.752230080700029
>500	3.9987949816734056E-4	0.05070089163431899
>1k	0.0015995179926693622	0.7775350383130212
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3268	0.2764966821244218	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2559	0.21651010084345026	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1415	0.1197193406383283	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1300	0.10998950023309313	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006683977321857198	0.0
2	0.0	0.0	0.0	0.02411308274340888	0.0
3	0.0	0.0	0.0	0.03299685006992794	0.0
4	0.0	0.0	0.0	0.054656320885060124	0.0
5	0.0	0.0	0.0	0.093744897121744	0.0
6	0.0	0.0	0.0	0.17877524153271215	0.0
7	0.0	0.0	0.0	0.2412154347419604	0.0
8	0.0	0.0	0.0	0.6240635028609961	0.0
9	0.0	0.0	0.0	0.7890477532106358	0.0
10	0.0	0.0	0.0	1.053953234156647	0.0
11	0.0	0.0	0.0	1.2255368545202723	0.0
12	0.0	0.0	0.0	1.4063426714419032	0.0
13	0.0	0.0	0.0	1.4770743808225693	0.0
14	0.0	0.0	0.0	1.5024565731840522	0.0
15	0.0	0.0	0.0	1.539176144800331	0.0
16	0.0	0.0	0.0	1.6228527722853534	0.0
17	0.0	0.0	0.0	1.7204049982613199	0.0
18	0.0	0.0	0.0	1.8794667370599467	0.0
19	0.0	0.0	0.0	1.9378457794913577	0.0
20	0.0	0.0	0.0	2.0111157081081723	0.0
21	0.0	0.0	0.0	2.0982612352159307	0.0
22	0.0	0.0	0.0	2.1831223650111555	0.0
23	0.0	0.0	0.0	2.2871893536932357	0.0
24	0.0	0.0	0.0	2.366127971937448	0.0
25	0.0	0.0	0.0	2.4307679551513584	0.0
26	0.0	0.0	0.0	2.496761655291214	0.0
27	8.460730787161011E-5	0.0	0.0	2.5651243600514753	0.0
28	8.460730787161011E-5	0.0	0.0	2.641017115212309	0.0
29	8.460730787161011E-5	0.0	0.0	2.7260474596232775	0.0
30	8.460730787161011E-5	0.0	0.0	2.836967640242958	0.0
31	8.460730787161011E-5	0.0	0.0	2.938581016996762	0.0
32	8.460730787161011E-5	0.0	0.0	3.027757119493439	0.0
33	8.460730787161011E-5	0.0	0.0	3.1189637973790347	0.0
34	8.460730787161011E-5	0.0	0.0	3.219646493746251	0.0
35	8.460730787161011E-5	0.0	0.0	3.3466420628615374	0.0
36	8.460730787161011E-5	0.0	0.0	3.442502142680072	0.0
37	8.460730787161011E-5	0.0	0.0	3.5445385559732334	0.0
38	8.460730787161011E-5	0.0	0.0	3.681010143570141	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAG	20	7.856571E-4	44.000004	6
GTCGAAA	20	7.856571E-4	44.000004	1
TAGGTCG	20	7.856571E-4	44.000004	6
CGTCTAG	20	7.856571E-4	44.000004	2
CGATCTA	20	7.856571E-4	44.000004	10
AGTACGT	20	7.856571E-4	44.000004	25
GTCGACC	25	4.442916E-5	44.0	35
TACGATA	25	4.442916E-5	44.0	1
AACGCAT	45	4.802132E-10	44.0	29
CGGTCAT	25	4.442916E-5	44.0	33
CGACTCA	25	4.442916E-5	44.0	35
GCGTATG	45	4.802132E-10	44.0	1
CGTTTTA	2135	0.0	43.0726	1
CGGTCTA	165	0.0	42.666664	31
AGGCGAT	935	0.0	42.117645	7
AGTACGG	105	0.0	41.904762	2
TAACGCC	90	0.0	41.555557	12
GTTTTAT	2490	0.0	41.349396	2
CCCGACG	80	0.0	41.250004	10
CGTTATT	1350	0.0	40.577778	1
>>END_MODULE