FastQCFastQC Report
Thu 26 May 2016
SRR1545785_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545785_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences451513
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC10250.22701450456576No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8020.17762500747486784No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.17607466451685777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC750.045.00000425
ACGTTAG207.02873E-445.0000042
ACCGCTC207.02873E-445.00000418
CGGCATA207.02873E-445.00000442
ATTACCG207.02873E-445.00000410
ATTCGAT207.02873E-445.00000414
TACGAAG351.2097371E-745.0000042
GTCGTAC207.02873E-445.00000420
TGTTACG207.02873E-445.0000041
ACGCTAC207.02873E-445.00000424
CTACCGA207.02873E-445.00000427
CTATTCG207.02873E-445.00000410
TTGTTCG207.02873E-445.00000428
CGCCGAC207.02873E-445.00000413
GCGTATG207.02873E-445.0000041
GCCCGAC207.02873E-445.0000049
GTTGCGA207.02873E-445.0000041
ATAGGCC253.8870312E-545.05
GCCTCTA253.8870312E-545.030
CATACTC253.8870312E-545.015