##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545785_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	451513
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.986049128153564	31.0	31.0	33.0	30.0	33.0
2	31.148697822654054	31.0	31.0	33.0	30.0	34.0
3	31.101518671666152	31.0	31.0	34.0	28.0	34.0
4	35.07906084653155	35.0	35.0	37.0	32.0	37.0
5	32.8030687931466	35.0	35.0	37.0	28.0	37.0
6	33.96233331044732	35.0	35.0	37.0	28.0	37.0
7	15.367333830919597	0.0	0.0	35.0	0.0	37.0
8	24.931897863405926	17.0	17.0	35.0	17.0	37.0
9	32.70558101317127	32.0	32.0	37.0	27.0	39.0
10	35.47617012134756	37.0	34.0	37.0	32.0	39.0
11	36.42563558524339	37.0	35.0	39.0	32.0	39.0
12	36.212955108712265	37.0	35.0	39.0	32.0	39.0
13	35.822078212587456	37.0	35.0	39.0	31.0	39.0
14	36.82298405583006	39.0	35.0	40.0	31.0	41.0
15	37.207225484094586	39.0	35.0	40.0	32.0	41.0
16	37.27925220314808	39.0	35.0	41.0	32.0	41.0
17	37.26233353192488	39.0	35.0	41.0	32.0	41.0
18	37.18147871711335	39.0	35.0	40.0	32.0	41.0
19	37.11809848221424	39.0	35.0	40.0	32.0	41.0
20	37.2111943620671	39.0	35.0	40.0	32.0	41.0
21	37.047774925638905	39.0	35.0	40.0	32.0	41.0
22	37.14208893210162	39.0	35.0	40.0	32.0	41.0
23	37.12853671987296	39.0	35.0	40.0	32.0	41.0
24	36.969099450071205	39.0	35.0	40.0	32.0	41.0
25	36.71762717795501	38.0	35.0	40.0	31.0	41.0
26	36.598543120574604	38.0	35.0	40.0	31.0	41.0
27	36.47452897258772	38.0	35.0	40.0	31.0	41.0
28	36.416202855731726	38.0	35.0	40.0	31.0	41.0
29	36.41278545689714	38.0	35.0	40.0	31.0	41.0
30	36.14087523504307	38.0	35.0	40.0	30.0	41.0
31	35.99313419547167	38.0	35.0	40.0	30.0	41.0
32	35.903384841632466	38.0	34.0	40.0	30.0	41.0
33	35.742344074257	38.0	34.0	40.0	29.0	41.0
34	35.58525889620011	38.0	34.0	40.0	28.0	41.0
35	35.43644369043638	38.0	34.0	40.0	27.0	41.0
36	35.22352401813458	38.0	34.0	40.0	26.0	41.0
37	35.061752374793194	38.0	34.0	40.0	26.0	41.0
38	34.95830241875649	38.0	34.0	40.0	25.0	41.0
39	34.871879657950046	38.0	34.0	40.0	25.0	41.0
40	34.7189361103667	37.0	33.0	40.0	24.0	41.0
41	34.569762110946975	37.0	33.0	40.0	24.0	41.0
42	34.5479620741817	37.0	33.0	40.0	24.0	41.0
43	34.45243880021173	37.0	33.0	40.0	23.0	41.0
44	34.241953166354016	37.0	33.0	40.0	23.0	41.0
45	34.13402050439301	37.0	33.0	40.0	23.0	41.0
46	34.12087138133343	36.0	33.0	40.0	23.0	41.0
47	34.05871591737115	36.0	33.0	40.0	23.0	41.0
48	33.998086433834686	36.0	33.0	40.0	23.0	41.0
49	33.93047154788456	36.0	33.0	40.0	23.0	41.0
50	33.78846899203345	36.0	33.0	40.0	23.0	41.0
51	33.58895978631845	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	9.0
15	32.0
16	68.0
17	145.0
18	334.0
19	606.0
20	976.0
21	1610.0
22	2374.0
23	3483.0
24	4848.0
25	6470.0
26	7572.0
27	8584.0
28	9461.0
29	11061.0
30	13713.0
31	17563.0
32	23240.0
33	34478.0
34	44166.0
35	46127.0
36	52715.0
37	79179.0
38	69201.0
39	13496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.51550675174358	23.35104415598222	28.186563842015623	13.946885250258575
2	33.236030856254416	22.89856548980871	30.917825178898507	12.947578475038371
3	31.151484010427165	23.175412446596223	32.548343015594234	13.124760527382378
4	27.319036218226273	26.912181930531347	29.783195611200565	15.985586240041815
5	21.226631348377566	36.27160236803813	28.199631018375992	14.30213526520831
6	20.69508519134554	41.96977717142142	27.603191934672978	9.73194570256006
7	38.655808359892184	56.69537754173191	3.8654479494499605	0.7833661489259446
8	88.41871662609935	3.2428745130262033	6.535360000708729	1.8030488601657098
9	84.69922239226777	4.359564397924312	8.069535096442406	2.8716781133655065
10	52.55042490470928	22.503006557950712	13.433500253591813	11.513068283748197
11	46.80330355936596	21.097066972601013	18.711310637788944	13.388318830244089
12	40.63005937813529	21.479558728098638	24.02079231384257	13.869589579923502
13	22.938652929151544	38.396458130773645	24.298303703326372	14.366585236748444
14	17.07149074334515	40.578233627824666	28.241268800676835	14.109006828153342
15	15.389147156338797	23.007975407131134	46.58581259011368	15.017064846416384
16	17.768259164187963	17.554533313548003	44.98187206126955	19.695335460994478
17	18.537672226491818	17.759843016701623	28.446135548699598	35.25634920810696
18	23.12491556167818	22.2040118446202	34.47785556562048	20.19321702808114
19	30.963671034942514	23.14108342395457	24.93505170393765	20.960193837165264
20	31.668191170575376	23.5922332247355	24.217021436813557	20.522554167875565
21	24.275048558956218	26.865450164225614	28.94933257735658	19.91016869946159
22	24.136182125431603	23.32003729682202	25.647766509491422	26.89601406825496
23	20.41004356463712	29.95041117310022	25.158744045021958	24.4808012172407
24	20.60295052412666	24.931286585325342	36.670704940943004	17.795057949604995
25	20.160216870832066	23.37540668817952	34.971529058963974	21.492847382024436
26	18.433577770739713	31.54261339097656	27.089142505309926	22.9346663329738
27	18.878969154819465	31.515814605559527	29.411777733974436	20.193438505646572
28	16.912248373800974	27.26433125956506	37.620400741506884	18.203019625127073
29	17.824957420938045	25.026300460894813	35.98102380219396	21.167718315973183
30	20.838381176178757	28.39873934969757	31.2336521872017	19.529227286921973
31	30.809744126968656	25.21101275046344	24.100302759831944	19.878940362735957
32	30.904979480103563	24.852883527163115	26.241547862409277	18.000589130324045
33	29.412442166670726	24.48345894802586	24.986877454248273	21.117221431055142
34	20.702615428570162	26.864785731529324	28.004730760797585	24.427868079102925
35	22.285737066263874	24.71379561607307	30.306990053442533	22.693477264220522
36	32.049575538245854	23.60507892353044	25.086985313822634	19.25836022440107
37	21.612888222487502	31.458008960982298	26.745187846197123	20.18391497033308
38	21.938238766104188	32.72486063524195	22.865343854994208	22.47155674365965
39	21.155537049874535	29.502805013366174	26.17288981712597	23.16876811963332
40	24.384901431409507	24.882782998496168	25.094958506178116	25.637357063916212
41	19.25592397118134	23.265110860595374	27.558896421586976	29.920068746636307
42	23.043633295165368	25.22806652300155	24.415243857873417	27.313056323959668
43	22.73644391191394	25.661055163417224	25.742337429930036	25.860163494738803
44	21.417102054647373	28.474484677074635	27.87583081771732	22.23258245056067
45	19.411622699678635	34.598782316345265	23.38448726836215	22.605107715613947
46	23.36654758556232	30.14974098198723	26.660140461071997	19.823570971378455
47	22.966116147264863	26.921041033148548	26.26413857408314	23.848704245503452
48	23.11096247505609	25.742115952364603	29.204031777601084	21.942889794978218
49	22.62947024781125	24.00085933295387	31.04805398737135	22.321616431863532
50	20.925421859392753	31.348820521225303	26.603442204321915	21.12231541506003
51	20.086464841543876	32.355657533670126	24.846903632896506	22.710973991889492
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	225.0
1	419.5
2	614.0
3	1965.0
4	3316.0
5	2549.0
6	1782.0
7	1845.0
8	1908.0
9	1992.0
10	2076.0
11	2048.0
12	2020.0
13	2004.0
14	1988.0
15	1878.0
16	1768.0
17	1747.0
18	1726.0
19	1686.0
20	1646.0
21	1675.0
22	1704.0
23	1918.5
24	2133.0
25	2354.5
26	3087.5
27	3599.0
28	4099.0
29	4599.0
30	5414.0
31	6229.0
32	7198.5
33	8168.0
34	8944.0
35	9720.0
36	11606.0
37	13492.0
38	18062.0
39	22632.0
40	29051.5
41	35471.0
42	38783.5
43	42096.0
44	41193.0
45	40290.0
46	39309.0
47	38328.0
48	36813.5
49	35299.0
50	33219.5
51	31140.0
52	28869.5
53	26599.0
54	24670.0
55	22741.0
56	21208.0
57	19675.0
58	18372.5
59	17070.0
60	15493.0
61	13916.0
62	12148.5
63	10381.0
64	9071.5
65	7762.0
66	6693.5
67	5625.0
68	4707.0
69	3789.0
70	3232.0
71	2675.0
72	2412.5
73	2150.0
74	1717.0
75	974.0
76	664.0
77	493.5
78	323.0
79	244.5
80	166.0
81	121.5
82	77.0
83	60.0
84	43.0
85	29.5
86	16.0
87	12.0
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	451513.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.972048703347276
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.64662683522472	22.963919720348823
2	8.000266516406409	5.2757035443742115
3	4.3364919501738575	4.289490713484175
4	2.8235313192262286	3.7239044869181432
5	2.128180450454413	3.508523473094721
6	1.7156004839747256	3.3940117627860484
7	1.4621733737535376	3.3747596184497466
8	1.1421651128339851	3.0127618982101025
9	1.031326133260697	3.0604441945415872
>10	7.610582835809994	44.20790306091263
>50	0.08310886200117512	1.7421537027235388
>100	0.017951514192253826	0.8716249052508859
>500	0.001329741792018802	0.35009682436762196
>1k	6.64870896009401E-4	0.22470209453776613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1025	0.22701450456576	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	802	0.17762500747486784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	795	0.17607466451685777	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007087282093760312	0.0
2	0.0	0.0	0.0	0.027020262982461192	0.0
3	0.0	0.0	0.0	0.03587936559966158	0.0
4	0.0	0.0	0.0	0.06024189779696266	0.0
5	0.0	0.0	0.0	0.10852400706070478	0.0
6	0.0	0.0	0.0	0.17075920294653754	0.0
7	0.0	0.0	0.0	0.2130614179436694	0.0
8	0.0	0.0	0.0	0.5406267372146538	0.0
9	0.0	0.0	0.0	0.6797146483047	0.0
10	0.0	0.0	0.0	0.8859102617200391	0.0
11	0.0	0.0	0.0	1.041830467782766	0.0
12	0.0	0.0	0.0	1.1835761096579722	0.0
13	0.0	0.0	0.0	1.2402743664080547	0.0
14	0.0	0.0	0.0	1.2590999594696055	0.0
15	0.0	0.0	0.0	1.294314892372977	0.0
16	0.0	0.0	0.0	1.3696172646191804	0.0
17	0.0	0.0	0.0	1.4531143067862942	0.0
18	0.0	0.0	0.0	1.5924236954417703	0.0
19	0.0	0.0	0.0	1.6460212662758327	0.0
20	0.0	0.0	0.0	1.7080349845962353	0.0
21	0.0	0.0	0.0	1.7804581484918485	0.0
22	0.0	0.0	0.0	1.858196773957782	0.0
23	0.0	0.0	0.0	1.9543180373544062	0.0
24	0.0	0.0	0.0	2.0260767685537293	0.0
25	0.0	0.0	0.0	2.0825535477383816	0.0
26	0.0	0.0	0.0	2.136151118572444	0.0
27	0.0	0.0	0.0	2.199272224719997	0.0
28	0.0	0.0	0.0	2.2728027764427603	0.0
29	0.0	0.0	0.0	2.3487695813852536	0.0
30	0.0	0.0	0.0	2.446884142870748	0.0
31	0.0	0.0	0.0	2.538797332524202	0.0
32	0.0	0.0	0.0	2.623180284953036	0.0
33	0.0	0.0	0.0	2.7104424457324594	0.0
34	0.0	0.0	0.0	2.8003623372970434	0.0
35	0.0	0.0	0.0	2.9113225975774784	0.0
36	0.0	0.0	0.0	3.0003565788803424	0.0
37	0.0	0.0	0.0	3.088061694790626	0.0
38	0.0	0.0	0.0	3.200793775594501	0.0
39	0.0	0.0	0.0	3.3890497062100096	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	75	0.0	45.000004	25
ACGTTAG	20	7.02873E-4	45.000004	2
ACCGCTC	20	7.02873E-4	45.000004	18
CGGCATA	20	7.02873E-4	45.000004	42
ATTACCG	20	7.02873E-4	45.000004	10
ATTCGAT	20	7.02873E-4	45.000004	14
TACGAAG	35	1.2097371E-7	45.000004	2
GTCGTAC	20	7.02873E-4	45.000004	20
TGTTACG	20	7.02873E-4	45.000004	1
ACGCTAC	20	7.02873E-4	45.000004	24
CTACCGA	20	7.02873E-4	45.000004	27
CTATTCG	20	7.02873E-4	45.000004	10
TTGTTCG	20	7.02873E-4	45.000004	28
CGCCGAC	20	7.02873E-4	45.000004	13
GCGTATG	20	7.02873E-4	45.000004	1
GCCCGAC	20	7.02873E-4	45.000004	9
GTTGCGA	20	7.02873E-4	45.000004	1
ATAGGCC	25	3.8870312E-5	45.0	5
GCCTCTA	25	3.8870312E-5	45.0	30
CATACTC	25	3.8870312E-5	45.0	15
>>END_MODULE