FastQCFastQC Report
Thu 26 May 2016
SRR1545784_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545784_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1485097
Sequences flagged as poor quality0
Sequence length50
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83890.5648789271003847No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.2432837720364394No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA207.8572263E-444.01
ACGTTAG408.314601E-944.02
CGCTTAG254.4434702E-544.02
CGTTTTA49250.043.597971
AGGCGAT9800.041.979597
CGTTATT25300.041.9130441
GTTTTAT55800.041.4372752
GTTATTT27600.041.0507242
AGGTACC6000.039.67
CGCATCG1000.039.621
TTACTGG25200.039.1984140
TTACGGG3200.039.18753
TCGTTAG452.3517714E-839.111112
CGGTCTA3350.038.7462731
ACGTAAC404.1256317E-738.516
CGTTTTT22800.038.307021
CGTTAAG1150.038.260872
ACATACG2600.038.07692317
CGAAGGA3300.038.04
GCCAGTA10850.037.91705325
Produced by FastQC (version 0.11.5)