##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545784_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1485097
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49986229855693	31.0	31.0	33.0	30.0	34.0
2	31.691328579884008	31.0	31.0	34.0	30.0	34.0
3	31.719852642621998	31.0	31.0	34.0	30.0	34.0
4	35.644547123857905	37.0	35.0	37.0	33.0	37.0
5	27.332814624229933	35.0	28.0	37.0	0.0	37.0
6	31.45997601503471	35.0	29.0	37.0	17.0	37.0
7	14.768520170736322	0.0	0.0	32.0	0.0	37.0
8	24.96726005102697	17.0	17.0	35.0	17.0	37.0
9	33.13809064323745	32.0	32.0	37.0	28.0	39.0
10	35.98744593787477	37.0	35.0	39.0	32.0	39.0
11	36.97946060089004	38.0	37.0	39.0	34.0	39.0
12	37.105704206526575	39.0	37.0	39.0	34.0	39.0
13	36.735924993451604	39.0	35.0	39.0	33.0	39.0
14	38.05176092874741	40.0	37.0	41.0	33.0	41.0
15	38.27130753075388	40.0	37.0	41.0	33.0	41.0
16	38.30700284223859	40.0	37.0	41.0	34.0	41.0
17	38.30864650591847	40.0	37.0	41.0	34.0	41.0
18	38.26288383856408	40.0	37.0	41.0	34.0	41.0
19	38.20726794276737	40.0	37.0	41.0	34.0	41.0
20	38.23873659431	40.0	37.0	41.0	34.0	41.0
21	38.114144732633626	40.0	37.0	41.0	34.0	41.0
22	38.00862098569993	40.0	36.0	41.0	34.0	41.0
23	37.88515497640895	40.0	36.0	41.0	33.0	41.0
24	37.71206863928754	40.0	35.0	41.0	33.0	41.0
25	37.531128943092604	39.0	35.0	41.0	33.0	41.0
26	37.282615883002926	39.0	35.0	40.0	33.0	41.0
27	37.090343593718124	39.0	35.0	40.0	32.0	41.0
28	37.09207681383775	39.0	35.0	40.0	32.0	41.0
29	36.96565476867841	39.0	35.0	40.0	32.0	41.0
30	36.737390217608684	38.0	35.0	40.0	31.0	41.0
31	36.56395844850538	38.0	35.0	40.0	31.0	41.0
32	36.32840211784146	38.0	35.0	40.0	31.0	41.0
33	36.07981431515921	38.0	35.0	40.0	30.0	41.0
34	35.740153000107064	38.0	35.0	40.0	29.0	41.0
35	35.4980368285708	38.0	35.0	40.0	27.0	41.0
36	35.288826925109944	38.0	35.0	40.0	26.0	41.0
37	35.19833115277992	38.0	34.0	40.0	26.0	41.0
38	34.97757722222858	38.0	34.0	40.0	25.0	41.0
39	34.870035425295455	38.0	34.0	40.0	25.0	41.0
40	34.62510462279568	38.0	34.0	40.0	24.0	41.0
41	34.39476613312127	37.0	34.0	40.0	23.0	40.0
42	34.3797374851609	37.0	34.0	40.0	23.0	40.0
43	34.25376120213023	37.0	33.0	40.0	23.0	40.0
44	34.018052692854404	37.0	33.0	40.0	23.0	40.0
45	33.80516828193714	36.0	33.0	39.0	23.0	40.0
46	33.739410287678176	36.0	33.0	39.0	23.0	40.0
47	33.535909102233724	36.0	33.0	39.0	23.0	40.0
48	33.42938138047548	36.0	33.0	39.0	22.0	40.0
49	33.28174051930615	35.0	33.0	39.0	22.0	40.0
50	33.371036370014885	36.0	33.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	6.0
13	6.0
14	16.0
15	45.0
16	107.0
17	248.0
18	597.0
19	1295.0
20	2392.0
21	4034.0
22	6185.0
23	9861.0
24	14602.0
25	21317.0
26	26761.0
27	28608.0
28	28798.0
29	31051.0
30	36729.0
31	47555.0
32	64824.0
33	108258.0
34	119409.0
35	154960.0
36	216571.0
37	366943.0
38	153675.0
39	40241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.31291222054856	22.993043552037342	24.21053978292327	17.48350444449083
2	35.29203816316375	23.95392354842815	28.488711511773307	12.265326776634792
3	30.38131515988518	24.046106079266202	33.48219005223228	12.09038870861634
4	26.683980911684557	26.474162967132784	31.890307501799548	14.951548619383113
5	16.97377343028772	47.61884240558024	24.220236119256857	11.187148044875183
6	20.450516026899255	39.89665321524453	29.633417884488356	10.019412873367868
7	38.02041213469558	56.31611941846223	4.757534356341707	0.9059340905004858
8	85.95721356921467	2.7805591149938356	9.574324101388664	1.68790321440283
9	81.79371448464309	4.3317035856917085	11.055102797999053	2.819479131666147
10	44.35467851594879	25.639402678747587	17.126894741555603	12.879024063748023
11	37.02936575860028	23.68303215210858	23.385879844885554	15.901722244405583
12	33.531345090590044	21.15740587988529	28.877170986137607	16.434078043387064
13	24.735353986978627	28.280913637291032	29.328656646670215	17.655075729060123
14	19.805507653708815	31.48669750191402	31.318425665124906	17.389369179252263
15	18.473136771537483	22.907863930773544	40.0136826079374	18.60531668975158
16	21.823894331481377	18.81702003303488	38.40806358103208	20.951022054451663
17	21.77689403453108	18.92226568365568	30.92457933724194	28.376260944571296
18	23.47644631966801	22.344331717052825	32.949362903567916	21.229859059711252
19	28.09654857561493	24.445675939012737	27.155599937243156	20.302175548129178
20	29.365354586266086	23.63892728892456	26.546683482627735	20.449034642181623
21	25.855213497838864	24.273767976098533	29.921008526715763	19.950009999346843
22	24.317132146923736	21.621685317524715	28.56581085275911	25.495371682792438
23	22.744036248137327	26.07203435196489	27.868482664768703	23.315446735129086
24	23.12380942120279	24.25336526839661	33.087603031990504	19.535222278410096
25	23.810767916169787	22.75986013034839	31.281189040177175	22.148182913304655
26	21.30621770833824	26.645868923040045	28.5152417653527	23.532671603269012
27	21.138888570914897	25.36460581362699	30.76842792086982	22.7280776945883
28	20.651445663145235	25.01607639096975	33.35378093148124	20.978697014403775
29	21.420621009940767	24.97143284243386	32.74836593165295	20.859580215972425
30	23.118287896346164	24.532538952001115	32.338830392896895	20.010342758755826
31	28.22906517217394	23.65737726222597	27.079712638299046	21.033844927301047
32	27.68485829545141	24.563311352726455	27.810573989443117	19.941256362379022
33	27.037291166839605	23.76740374534458	26.79818220628013	22.397122881535683
34	22.323727002343954	25.840399650662548	28.798186246420265	23.03768710057323
35	22.731511813706444	24.762422925909892	29.722435638884193	22.783629621499472
36	28.13782534070165	25.39847565512556	25.841746364042212	20.621952640130576
37	23.316591441501803	28.50817152010946	25.990558192495172	22.18467884589357
38	24.14354079228495	28.995951106224037	24.60068264901215	22.25982545247886
39	23.241781513261422	26.6910511569278	24.87972166127869	25.18744566853209
40	24.500083159551195	23.986042662533155	27.673343896055275	23.840530281860378
41	21.950216046493935	23.426617924620412	28.435044983593667	26.18812104529199
42	23.460757108794912	26.313634732276746	25.13815595883636	25.08745220009198
43	23.44129710045876	25.390799388861467	26.077219198476598	25.090684312203177
44	22.82564707894501	26.280640254474964	28.292899386370046	22.60081328020998
45	22.379076922248174	28.34414183046629	26.15505923182122	23.12172201546431
46	24.12576417567337	26.844037796857712	27.667014343170848	21.36318368429806
47	23.388371264637932	26.905111248625513	27.473693637519975	22.232823849216583
48	23.457457661014736	25.72141752356917	27.93460629171024	22.88651852370586
49	23.18394017360482	24.74787842140951	29.237551486535896	22.83062991844977
50	21.97513024401773	26.472210232732273	28.38447589618725	23.168183627062746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	978.0
1	1835.5
2	2693.0
3	14523.0
4	26353.0
5	18833.0
6	11313.0
7	11095.5
8	10878.0
9	11016.5
10	11155.0
11	10872.5
12	10590.0
13	10258.5
14	9927.0
15	9356.0
16	8785.0
17	8219.0
18	7653.0
19	7406.5
20	7160.0
21	7014.0
22	6868.0
23	7341.5
24	7815.0
25	8972.0
26	10129.0
27	11443.5
28	12758.0
29	14807.5
30	16857.0
31	19283.0
32	21709.0
33	24814.5
34	27920.0
35	30628.0
36	33336.0
37	39574.5
38	45813.0
39	55688.5
40	65564.0
41	76101.0
42	86638.0
43	91032.0
44	95426.0
45	101129.5
46	106833.0
47	111237.0
48	115641.0
49	112899.5
50	110158.0
51	101431.5
52	92705.0
53	88030.5
54	83356.0
55	82853.5
56	82351.0
57	82005.5
58	81660.0
59	76971.0
60	72282.0
61	65691.0
62	59100.0
63	51969.0
64	44838.0
65	38309.5
66	31781.0
67	27703.5
68	23626.0
69	20361.5
70	17097.0
71	14238.0
72	11379.0
73	9059.0
74	6739.0
75	5320.5
76	3902.0
77	2868.5
78	1835.0
79	1285.5
80	736.0
81	561.0
82	386.0
83	300.5
84	215.0
85	162.5
86	110.0
87	69.0
88	28.0
89	22.5
90	17.0
91	9.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1485097.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.749827748608148
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69421062774795	21.031405889993252
2	8.103809978347318	4.821739019265692
3	4.115292829961402	3.6728775847930164
4	2.675921839395586	3.184328551630294
5	1.9322614947301395	2.874222331674488
6	1.5576431924710488	2.780377000188347
7	1.2555760352159244	2.6147219541067876
8	1.0213698809177278	2.430846241993505
9	0.8408187336716413	2.2512791245080743
>10	7.480291588842651	45.08194561855596
>50	0.2799534205141621	5.15738309669239
>100	0.03721217054313985	1.962675501033846
>500	0.004510566113090584	0.9247269479577716
>1k	8.457311462044845E-4	0.5079037181962873
>5k	2.819103820681615E-4	0.7035674194102798
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8389	0.5648789271003847	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3613	0.2432837720364394	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00430948281492724	0.0
2	0.0	0.0	0.0	0.016766581576826294	0.0
3	0.0	0.0	0.0	0.024375512171932203	0.0
4	0.0	0.0	0.0	0.03535122621620002	0.0
5	0.0	0.0	0.0	0.06639296961747279	0.0
6	0.0	0.0	0.0	0.12551368698475587	0.0
7	0.0	0.0	0.0	0.16746380876131323	0.0
8	0.0	0.0	0.0	0.3943176775658425	0.0
9	0.0	0.0	0.0	0.5141078326870231	0.0
10	0.0	0.0	0.0	0.7316020435028823	0.0
11	6.733566898323813E-5	0.0	0.0	0.8745556687542969	0.0
12	6.733566898323813E-5	0.0	0.0	1.0224247978414878	0.0
13	6.733566898323813E-5	0.0	0.0	1.0850469699958993	0.0
14	1.3467133796647626E-4	0.0	0.0	1.112991272623943	0.0
15	1.3467133796647626E-4	0.0	0.0	1.1467937784535287	0.0
16	1.3467133796647626E-4	0.0	0.0	1.2043657754341972	0.0
17	1.3467133796647626E-4	0.0	0.0	1.2694120316720052	0.0
18	1.3467133796647626E-4	0.0	0.0	1.3723009338783931	0.0
19	1.3467133796647626E-4	0.0	0.0	1.4246880843473524	0.0
20	1.3467133796647626E-4	0.0	0.0	1.4815867246381886	0.0
21	1.3467133796647626E-4	0.0	0.0	1.5497304216492256	0.0
22	1.3467133796647626E-4	0.0	0.0	1.6180761256672123	0.0
23	1.3467133796647626E-4	0.0	0.0	1.6989462641160813	0.0
24	1.3467133796647626E-4	0.0	0.0	1.7615011006015096	0.0
25	1.3467133796647626E-4	0.0	0.0	1.8118008453319885	0.0
26	1.3467133796647626E-4	0.0	0.0	1.8589358136202552	0.0
27	1.3467133796647626E-4	0.0	0.0	1.9165078106009237	0.0
28	1.3467133796647626E-4	0.0	0.0	1.9791973184243183	0.0
29	1.3467133796647626E-4	0.0	0.0	2.044916931351959	0.0
30	1.3467133796647626E-4	0.0	0.0	2.1336653430718666	0.0
31	1.3467133796647626E-4	0.0	0.0	2.2052431592010486	0.0
32	1.3467133796647626E-4	0.0	0.0	2.27991841610346	0.0
33	1.3467133796647626E-4	0.0	0.0	2.3593071698346977	0.0
34	1.3467133796647626E-4	0.0	0.0	2.4307503146259135	0.0
35	1.3467133796647626E-4	0.0	0.0	2.5250875868714298	0.0
36	1.3467133796647626E-4	0.0	0.0	2.599493501097908	0.0
37	1.3467133796647626E-4	0.0	0.0	2.6866931924312016	0.0
38	1.3467133796647626E-4	0.0	0.0	2.7906594653413213	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAA	20	7.8572263E-4	44.0	1
ACGTTAG	40	8.314601E-9	44.0	2
CGCTTAG	25	4.4434702E-5	44.0	2
CGTTTTA	4925	0.0	43.59797	1
AGGCGAT	980	0.0	41.97959	7
CGTTATT	2530	0.0	41.913044	1
GTTTTAT	5580	0.0	41.437275	2
GTTATTT	2760	0.0	41.050724	2
AGGTACC	600	0.0	39.6	7
CGCATCG	100	0.0	39.6	21
TTACTGG	2520	0.0	39.19841	40
TTACGGG	320	0.0	39.1875	3
TCGTTAG	45	2.3517714E-8	39.11111	2
CGGTCTA	335	0.0	38.74627	31
ACGTAAC	40	4.1256317E-7	38.5	16
CGTTTTT	2280	0.0	38.30702	1
CGTTAAG	115	0.0	38.26087	2
ACATACG	260	0.0	38.076923	17
CGAAGGA	330	0.0	38.0	4
GCCAGTA	1085	0.0	37.917053	25
>>END_MODULE