FastQCFastQC Report
Thu 26 May 2016
SRR1545783_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545783_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences562374
Sequences flagged as poor quality0
Sequence length51
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21200.37697333091501384No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19730.3508341424034539No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC6090.10829092383360539No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATG207.030212E-445.01
ACGGGTA207.030212E-445.05
ACGTAAC207.030212E-445.016
CGTTTTA12850.043.9494171
TAAGGTA1250.043.25
AGGCGAT4300.042.9069757
AATAGGC950.042.631584
GTAAGGT1500.042.04
GTTTTAT15200.041.2993432
CGTTTTT17900.041.103351
ATAGGCC556.002665E-1140.9090925
CGTTATT3250.040.8461531
ATACTAT2550.040.58823445
CGCATCG501.0786607E-940.521
CAACCCG501.0786607E-940.523
TCGTCCC1900.040.26315738
TCACGAC1400.040.1785725
CACGACG1400.040.1785726
CGGTCTA1350.040.00000431
GGCGATT3350.039.626868
Produced by FastQC (version 0.11.5)