##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545783_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	562374
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.984734358274032	31.0	31.0	33.0	30.0	33.0
2	31.14915874489219	31.0	31.0	33.0	30.0	34.0
3	31.05922037647544	31.0	31.0	34.0	28.0	34.0
4	35.05172892061155	35.0	35.0	37.0	32.0	37.0
5	32.85651185865634	35.0	35.0	37.0	28.0	37.0
6	34.02252060016999	35.0	35.0	37.0	28.0	37.0
7	14.839604960400019	0.0	0.0	35.0	0.0	37.0
8	24.652306116570113	17.0	17.0	35.0	17.0	37.0
9	32.56906258112929	32.0	32.0	37.0	27.0	39.0
10	35.40733568763847	37.0	34.0	37.0	32.0	39.0
11	36.39062083239979	37.0	35.0	39.0	32.0	39.0
12	36.40360151785111	37.0	35.0	39.0	32.0	39.0
13	36.223330025925804	38.0	35.0	39.0	32.0	39.0
14	37.273376791956956	39.0	36.0	40.0	32.0	41.0
15	37.52623876637256	39.0	36.0	41.0	32.0	41.0
16	37.518452133277854	39.0	36.0	41.0	32.0	41.0
17	37.42793941398429	39.0	36.0	41.0	32.0	41.0
18	37.34809219487387	39.0	36.0	40.0	32.0	41.0
19	37.28805030104521	39.0	36.0	40.0	32.0	41.0
20	37.39460572501574	39.0	35.0	40.0	32.0	41.0
21	37.280349020402795	39.0	35.0	40.0	32.0	41.0
22	37.30968714769886	39.0	35.0	40.0	32.0	41.0
23	37.25613026206759	39.0	35.0	40.0	32.0	41.0
24	37.05630950221739	39.0	35.0	40.0	32.0	41.0
25	36.81630729727903	38.0	35.0	40.0	31.0	41.0
26	36.689274397465034	38.0	35.0	40.0	31.0	41.0
27	36.6136539029187	38.0	35.0	40.0	31.0	41.0
28	36.49376038010292	38.0	35.0	40.0	31.0	41.0
29	36.416086092173536	38.0	35.0	40.0	30.0	41.0
30	36.10792461955923	38.0	35.0	40.0	30.0	41.0
31	35.972873923758925	38.0	35.0	40.0	30.0	41.0
32	35.8278441037459	38.0	34.0	40.0	29.0	41.0
33	35.621613019094056	38.0	34.0	40.0	27.0	41.0
34	35.356894166515524	38.0	34.0	40.0	26.0	41.0
35	35.12466792561534	38.0	34.0	40.0	25.0	41.0
36	34.913970062627364	38.0	34.0	40.0	24.0	41.0
37	34.80132260737516	38.0	34.0	40.0	24.0	41.0
38	34.61509422555097	38.0	33.0	40.0	23.0	41.0
39	34.498975770572606	38.0	33.0	40.0	23.0	41.0
40	34.32756315192381	38.0	33.0	40.0	23.0	41.0
41	34.147361008865985	37.0	33.0	40.0	22.0	41.0
42	34.085053718699655	37.0	33.0	40.0	21.0	41.0
43	33.99253166042527	37.0	33.0	40.0	21.0	41.0
44	33.65087824117047	37.0	33.0	40.0	20.0	41.0
45	33.583632956004365	37.0	33.0	40.0	20.0	41.0
46	33.56650378573689	36.0	32.0	40.0	20.0	41.0
47	33.458102614985755	36.0	32.0	40.0	20.0	41.0
48	33.402740880623924	36.0	32.0	40.0	20.0	41.0
49	33.284223666101205	36.0	32.0	40.0	20.0	41.0
50	33.16304274379683	36.0	32.0	39.0	20.0	40.0
51	33.01078108162895	35.0	32.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	2.0
13	7.0
14	9.0
15	26.0
16	82.0
17	172.0
18	449.0
19	827.0
20	1472.0
21	2339.0
22	3455.0
23	5146.0
24	7115.0
25	9531.0
26	11289.0
27	11981.0
28	12568.0
29	14106.0
30	16953.0
31	20925.0
32	27838.0
33	39568.0
34	49387.0
35	56555.0
36	70552.0
37	101379.0
38	81638.0
39	17001.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.36258788635321	23.68210479147329	24.198131492565444	16.757175829608055
2	34.82486743697255	23.9948859655674	28.892160732893057	12.288085864566996
3	30.869314726498736	23.95416573312422	32.92915390825323	12.247365632123817
4	27.145280542841597	27.20751670596436	30.523281659536178	15.123921091657866
5	20.70401547724468	36.39055148353231	29.17186783172764	13.733565207495369
6	20.48921180566669	40.674888952903224	28.98124735496307	9.854651886467012
7	36.135916667555755	57.9301319051023	5.22997151361905	0.7039799137228961
8	86.42326992357398	3.1950267971136648	8.726754793073649	1.6549484862386954
9	82.17538506403213	4.505542574870105	10.21953361997532	3.0995387411224558
10	44.49369992211589	26.312382862650125	16.317788517961358	12.876128697272634
11	37.38721918154111	24.122025555946756	22.657697546472633	15.833057716039503
12	33.72808842514056	21.56323016355664	28.20756293854268	16.50111847276012
13	24.98550786487284	28.935014776643303	28.46077521364786	17.618702144836
14	19.69490054661133	32.53208718753001	30.44788699335318	17.325125272505485
15	18.311835184414644	23.501442100808358	39.4975585642295	18.689164150547498
16	21.772877124475883	19.09707774541497	38.03803163019627	21.09201349991287
17	21.607328930569338	19.51690512008023	30.218324460234648	28.657441489115786
18	23.49023959144626	22.646317219501615	32.52675265926234	21.336690529789784
19	28.315498227158436	24.784396149181863	26.495534999839965	20.404570623819733
20	29.825169726907717	23.880193607812593	25.72807419973185	20.56656246554784
21	26.017916902275	24.883440557351513	29.211343340908364	19.887299199465126
22	24.24738697023689	21.93842531838243	28.027611518313435	25.786576193067244
23	22.841916589316007	26.653259218953934	27.111317379537457	23.393506812192598
24	23.007642600831474	24.871526777553726	32.70634844427374	19.414482177341057
25	23.75749945765629	23.291795139889114	30.800499311845854	22.15020609060874
26	21.15833946804084	27.263529252774845	28.100694555580453	23.477436723603866
27	21.238001756837978	26.160882259848428	30.006188052790495	22.594927930523102
28	20.56105011967125	25.73963234431179	32.9040816253952	20.795235910621756
29	21.306283718664094	25.42240572999463	32.418461735428735	20.85284881591254
30	22.97616888405225	25.307535554630906	31.885008908662204	19.83128665265464
31	28.608186011444342	23.992218701433565	26.401647302329057	20.99794798479304
32	28.244193365980646	24.846632312304624	27.201648724869926	19.707525596844803
33	27.597826357548534	23.92322546917176	26.074285084303327	22.404663088976378
34	22.373011554588228	26.144700857436508	28.09820510905554	23.384082478919723
35	22.803330168179894	24.807512438341746	29.50492021323888	22.884237180239484
36	28.44245999992887	25.53994316949219	25.518427238812606	20.499169591766332
37	23.22689171263252	29.03583736090218	25.728963287776462	22.008307638688844
38	23.827737413180554	29.622457652736433	24.22693794521084	22.322866988872175
39	23.133004015121607	27.258550359725024	24.482106214014163	25.126339411139202
40	24.59555384850651	24.236184460874792	27.418941842972828	23.74931984764587
41	21.85680703588715	23.625914427053882	28.238503202495135	26.278775334563832
42	23.332871007550136	26.47473033959607	24.801822274856235	25.390576377997558
43	23.289839146190968	25.42151664195002	25.827474243119347	25.461169968739668
44	22.591549395953585	26.68722238225807	28.2077407561516	22.513487465636747
45	22.362164680443975	28.85890884002461	25.981642110054874	22.79728436947654
46	24.169680675137897	27.22885481903502	27.364529654642638	21.236934851184444
47	23.39368462980152	27.239701693179274	26.986845053291937	22.37976862372727
48	23.215689203270422	26.073218178649793	27.843392475470065	22.867700142609724
49	23.149185417533527	25.109091103073755	29.199963013937346	22.541760465455376
50	22.05436239939969	27.109005750621474	27.95630665713564	22.880325192843195
51	21.943759846650092	28.618321615152905	27.111495197146386	22.326423341050617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	415.0
1	699.0
2	983.0
3	4526.0
4	8069.0
5	5873.0
6	3677.0
7	3654.0
8	3631.0
9	3684.0
10	3737.0
11	3693.5
12	3650.0
13	3482.0
14	3314.0
15	3183.5
16	3053.0
17	2904.0
18	2755.0
19	2612.5
20	2470.0
21	2454.5
22	2439.0
23	2662.5
24	2886.0
25	3243.0
26	4102.0
27	4604.0
28	5348.5
29	6093.0
30	7001.5
31	7910.0
32	8922.5
33	9935.0
34	11227.0
35	12519.0
36	14386.5
37	16254.0
38	19346.5
39	22439.0
40	26520.0
41	30601.0
42	33186.5
43	35772.0
44	37187.5
45	38603.0
46	40141.0
47	41679.0
48	41039.0
49	40399.0
50	39209.5
51	38020.0
52	35650.5
53	33281.0
54	32515.5
55	31750.0
56	31377.5
57	31005.0
58	29513.5
59	28022.0
60	25925.0
61	23828.0
62	21411.5
63	18995.0
64	16620.5
65	14246.0
66	12125.5
67	10005.0
68	8814.5
69	7624.0
70	6639.5
71	5655.0
72	4707.0
73	3759.0
74	3020.5
75	1770.0
76	1258.0
77	933.0
78	608.0
79	447.0
80	286.0
81	209.5
82	133.0
83	104.0
84	75.0
85	54.5
86	34.0
87	22.5
88	11.0
89	8.5
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	562374.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.02098705165003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.58153299148987	28.132821768262158
2	10.179782347088755	8.351694396970869
3	5.363675401058203	6.60069777528187
4	3.510795259204849	5.760651474753457
5	2.468539053818755	5.063095428159579
6	1.9285475756935837	4.746655507861053
7	1.4361099977338858	4.123745473525082
8	1.1587513987741713	3.8026500900157316
9	0.9216876414351929	3.402768312448101
>10	4.398332581686036	26.758660582989087
>50	0.03411086290423315	0.988516091153054
>100	0.016839539890181013	1.4352169642947012
>500	4.3178307410720543E-4	0.10786710246272653
>1k	8.635661482144109E-4	0.724959031822561
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2120	0.37697333091501384	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1973	0.3508341424034539	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	609	0.10829092383360539	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0037341697873657033	0.0
2	0.0	0.0	0.0	0.019737754590361575	0.0
3	0.0	0.0	0.0	0.02773954699185951	0.0
4	0.0	0.0	0.0	0.04374313179485538	0.0
5	0.0	0.0	0.0	0.07468339574731407	0.0
6	0.0	0.0	0.0	0.11131382318528239	0.0
7	0.0	0.0	0.0	0.13851991735037536	0.0
8	0.0	0.0	0.0	0.34496616130902213	0.0
9	0.0	0.0	0.0	0.44827819209280656	0.0
10	0.0	0.0	0.0	0.6420993858179788	0.0
11	0.0	0.0	0.0	0.75821428444416	0.0
12	0.0	0.0	0.0	0.8864207804770491	0.0
13	0.0	0.0	0.0	0.9392326103269355	0.0
14	0.0	0.0	0.0	0.9637714403581958	0.0
15	0.0	0.0	0.0	0.9993349621426311	0.0
16	0.0	0.0	0.0	1.0541027856906613	0.0
17	0.0	0.0	0.0	1.1124269614171352	0.0
18	0.0	0.0	0.0	1.20186921870499	0.0
19	0.0	0.0	0.0	1.2505912435496662	0.0
20	0.0	0.0	0.0	1.299668903612187	0.0
21	0.0	0.0	0.0	1.362260701952793	0.0
22	0.0	0.0	0.0	1.420762695288189	0.0
23	0.0	0.0	0.0	1.4879777514607717	0.0
24	0.0	0.0	0.0	1.5466575624050898	0.0
25	0.0	0.0	0.0	1.5909341470267118	0.0
26	0.0	0.0	0.0	1.6360998196929446	0.0
27	0.0	0.0	0.0	1.69211236650343	0.0
28	0.0	0.0	0.0	1.7429682026551725	0.0
29	0.0	0.0	0.0	1.799869837510269	0.0
30	0.0	0.0	0.0	1.882555025659081	0.0
31	0.0	0.0	0.0	1.9478140881335197	0.0
32	0.0	0.0	0.0	2.0159182323507134	0.0
33	0.0	0.0	0.0	2.0865118230928172	0.0
34	0.0	0.0	0.0	2.15781668427061	0.0
35	0.0	0.0	0.0	2.2401462372015777	0.0
36	0.0	0.0	0.0	2.304160576413561	0.0
37	0.0	0.0	0.0	2.37350944389321	0.0
38	0.0	0.0	0.0	2.4668636885773525	0.0
39	0.0	0.0	0.0	2.606272693972339	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATG	20	7.030212E-4	45.0	1
ACGGGTA	20	7.030212E-4	45.0	5
ACGTAAC	20	7.030212E-4	45.0	16
CGTTTTA	1285	0.0	43.949417	1
TAAGGTA	125	0.0	43.2	5
AGGCGAT	430	0.0	42.906975	7
AATAGGC	95	0.0	42.63158	4
GTAAGGT	150	0.0	42.0	4
GTTTTAT	1520	0.0	41.299343	2
CGTTTTT	1790	0.0	41.10335	1
ATAGGCC	55	6.002665E-11	40.909092	5
CGTTATT	325	0.0	40.846153	1
ATACTAT	255	0.0	40.588234	45
CGCATCG	50	1.0786607E-9	40.5	21
CAACCCG	50	1.0786607E-9	40.5	23
TCGTCCC	190	0.0	40.263157	38
TCACGAC	140	0.0	40.17857	25
CACGACG	140	0.0	40.17857	26
CGGTCTA	135	0.0	40.000004	31
GGCGATT	335	0.0	39.62686	8
>>END_MODULE