FastQCFastQC Report
Thu 26 May 2016
SRR1545782_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545782_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1050800
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42570.40511990864103536No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18260.17377236391320897No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18120.1724400456794823No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13940.13266082984392844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC3200.044.025
TCAATCG207.8561745E-444.028
TTACGCG302.5275094E-644.013
ACGTATA207.8561745E-444.013
AATCGAT207.8561745E-444.024
CTTACGA254.4425782E-544.021
CGTTTTA27950.043.0554581
CGTTATT13050.041.6398471
CGACGGT3600.041.55555728
CGGTCTA3350.041.37313531
CACGACG3400.040.76470626
TAACGGG2000.040.73
AGGCGAT7300.040.383567
CAAGGAC9600.040.1041645
GTTATTT14800.039.6891862
GTTTTAT32800.039.6402442
TTACTGG20650.039.5254240
TATGGGC2850.039.3684234
CTCACGA3650.038.5753424
ATCACGG404.1240855E-738.52
Produced by FastQC (version 0.11.5)