FastQCFastQC Report
Thu 26 May 2016
SRR1545781_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545781_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences401344
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11130.2773182108116728No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10220.2546443948333599No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC5480.1365412214957742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC1300.045.00000425
CACGACG1300.045.00000426
GTACCGC207.0277933E-445.031
GACGTCA207.0277933E-445.09
CGCATCG207.0277933E-445.021
ATAGCGG207.0277933E-445.02
GGATCGT207.0277933E-445.08
GCGTTAA207.0277933E-445.01
ATGCGCG406.7957444E-945.036
ATACGAA207.0277933E-445.019
TGCGTAG207.0277933E-445.02
GTTAATC302.161958E-644.99999640
CGAGACA600.044.99999622
CGAAGGA1400.043.392864
CGACGGT1350.043.33333628
GACGGTC1350.043.33333629
ACGGTCT1350.043.33333630
ATAGGCG1200.043.1249965
CGTTTTA6600.042.2727281
CGTTATT1450.041.896551