##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545781_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	401344
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.957931350661777	31.0	31.0	33.0	30.0	33.0
2	31.136249701004626	31.0	31.0	33.0	30.0	34.0
3	31.068631896826663	31.0	31.0	34.0	28.0	34.0
4	35.055677922181474	35.0	35.0	37.0	32.0	37.0
5	32.78706047679796	35.0	35.0	37.0	28.0	37.0
6	33.9609586788391	35.0	35.0	37.0	28.0	37.0
7	15.078329313506618	0.0	0.0	35.0	0.0	37.0
8	24.78385624302344	17.0	17.0	35.0	17.0	37.0
9	32.63090017541062	32.0	32.0	37.0	27.0	39.0
10	35.38364844123744	37.0	34.0	37.0	32.0	39.0
11	36.374068131079575	37.0	35.0	39.0	32.0	39.0
12	36.320632674214636	37.0	35.0	39.0	32.0	39.0
13	36.08421204752033	37.0	35.0	39.0	32.0	39.0
14	37.066531454313505	39.0	36.0	40.0	31.0	41.0
15	37.37156404480944	39.0	36.0	41.0	32.0	41.0
16	37.38845230027109	39.0	36.0	41.0	32.0	41.0
17	37.325797819327065	39.0	35.0	41.0	32.0	41.0
18	37.25787852814543	39.0	36.0	40.0	32.0	41.0
19	37.20829512836868	39.0	36.0	40.0	32.0	41.0
20	37.32710094083878	39.0	35.0	40.0	32.0	41.0
21	37.194628548078455	39.0	35.0	40.0	32.0	41.0
22	37.251918553659706	39.0	35.0	40.0	32.0	41.0
23	37.2508870196141	39.0	35.0	40.0	32.0	41.0
24	37.067732419071916	39.0	35.0	40.0	32.0	41.0
25	36.811839718545684	38.0	35.0	40.0	31.0	41.0
26	36.69995066576303	38.0	35.0	40.0	31.0	41.0
27	36.61629923457184	38.0	35.0	40.0	31.0	41.0
28	36.528284962525916	38.0	35.0	40.0	31.0	41.0
29	36.47684280816457	38.0	35.0	40.0	31.0	41.0
30	36.17320802104928	38.0	35.0	40.0	30.0	41.0
31	36.03545337665444	38.0	35.0	40.0	30.0	41.0
32	35.93794849306331	38.0	35.0	40.0	29.0	41.0
33	35.75623156195184	38.0	34.0	40.0	29.0	41.0
34	35.542173297719664	38.0	34.0	40.0	27.0	41.0
35	35.360703037793016	38.0	34.0	40.0	26.0	41.0
36	35.17393308483496	38.0	34.0	40.0	25.0	41.0
37	35.06337206984532	38.0	34.0	40.0	25.0	41.0
38	34.90550500318928	38.0	34.0	40.0	24.0	41.0
39	34.776912075426566	38.0	34.0	40.0	24.0	41.0
40	34.63810347233296	38.0	33.0	40.0	23.0	41.0
41	34.45524786716632	37.0	33.0	40.0	23.0	41.0
42	34.42046224685058	37.0	33.0	40.0	23.0	41.0
43	34.31950396667198	37.0	33.0	40.0	23.0	41.0
44	34.092982578536116	37.0	33.0	40.0	23.0	41.0
45	34.01720967549035	37.0	33.0	40.0	23.0	41.0
46	33.96812958459576	37.0	33.0	40.0	23.0	41.0
47	33.87168115133153	37.0	33.0	40.0	23.0	41.0
48	33.80753916839419	36.0	33.0	40.0	22.0	41.0
49	33.6728890926487	36.0	33.0	40.0	22.0	41.0
50	33.55337814941795	36.0	32.0	40.0	22.0	41.0
51	33.387787035560514	36.0	32.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	5.0
14	14.0
15	20.0
16	60.0
17	146.0
18	270.0
19	558.0
20	940.0
21	1485.0
22	2266.0
23	3235.0
24	4648.0
25	6159.0
26	7219.0
27	7887.0
28	8680.0
29	10002.0
30	12136.0
31	15500.0
32	19962.0
33	28713.0
34	35834.0
35	39988.0
36	49725.0
37	72750.0
38	60544.0
39	12597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.99463801626535	24.55499521607399	27.973010684101418	14.47735608355924
2	34.83520371551587	22.917248046563547	29.66432785839579	12.583220379524796
3	31.679805852336152	23.04407191835433	32.16741747727635	13.108704752033168
4	27.913211608993784	26.75061792377611	29.31724405995854	16.018926407271568
5	21.021866528464358	36.03517182267581	28.362202997926968	14.580758650932864
6	20.060845558922022	42.17628767341732	28.168603492265987	9.594263275394674
7	37.54659344602137	57.325885026311596	4.3431569925051825	0.7843645351618562
8	87.54260684101419	3.1860448891723805	7.445981502152767	1.8253667676606602
9	83.25451483017062	4.519564264072716	8.987800988678043	3.2381199170786155
10	45.751524876415246	25.233465555732735	15.546762876734174	13.468246691117844
11	39.72228312868761	23.098638574390048	21.353253069685856	15.825825227236484
12	35.68136062828895	21.213472731621753	27.62243860628289	15.482728033806412
13	24.55723768139053	31.163291341093924	27.962296683144633	16.317174294370915
14	19.00838183702759	34.50730545367565	30.35849545527029	16.12581725402647
15	17.732419071918354	23.532929357359272	41.17988558443629	17.55476598628608
16	20.711160500717586	18.84567852017222	40.19195503109552	20.251205948014668
17	20.50111624940201	19.124242545048638	29.741070004783925	30.63357120076543
18	23.737741189602936	23.244897145590816	32.32414088662095	20.6932207781853
19	28.921573512996336	24.66936094721735	25.74948174134907	20.65958379843725
20	29.237262797002074	24.34021687131239	24.99850502312231	21.424015308563227
21	25.257385185775792	25.089449449848512	29.74430912135226	19.90885624302344
22	23.677692951682346	22.629963323233934	27.670527029181947	26.021816695901773
23	21.482817732419072	27.92168314463403	26.85975123584755	23.735747887099347
24	21.777826502950088	25.29326463084038	33.54454034444267	19.384368521766863
25	22.941167676606604	23.312669430712806	31.299085074150852	22.44707781852974
26	19.708529341412852	28.196758890129164	27.43880561313985	24.65590615531813
27	20.52603253069686	27.82226718226758	30.389391644075904	21.262308642959653
28	18.884547918992187	26.784254903524157	33.91853372667836	20.412663450805294
29	19.99157829692234	26.158607080210494	32.8042776271727	21.045536995694466
30	22.83901092329772	25.93261640886621	31.659374501674375	19.5689981661617
31	29.090505900175412	24.96686134587785	26.072396746930316	19.870236007016427
32	28.830629086270132	25.05282251634508	26.669640009567853	19.446908387816936
33	27.96653245096476	24.216881278902886	26.00337864774358	21.813207622388774
34	21.621601419231382	26.50793334396428	28.726229867644715	23.144235369159624
35	21.980395869877213	24.849754823792058	29.633182506777228	23.536666799553497
36	28.85454871631319	25.052573353532132	25.64682666241429	20.44605126774039
37	22.164776351459096	29.852694944984854	26.476040503906873	21.50648819964918
38	23.005700845160263	30.471864535161856	23.995375538191677	22.527059081486208
39	21.8585552543454	27.604997209376496	25.84864854090257	24.687798995375537
40	24.287394354967308	23.65053420507096	27.836220299792696	24.225851140169034
41	20.836489395630682	23.697875139531174	27.80806490192952	27.657570562908628
42	22.80562310636262	26.391574310317335	24.723180114814227	26.07962246850582
43	22.15979309520013	25.17840057407112	26.46856561951842	26.193240711210336
44	21.870764232179877	26.691317174294372	28.38637179078297	23.051546802742784
45	21.232159942592887	29.856183224366127	25.37349505660979	23.538161776431192
46	23.720798118322435	27.695941636102695	27.315719183543298	21.267541062031576
47	23.031115452081007	27.593784882793816	26.393069287195026	22.982030377930155
48	22.432875538191677	26.772544251315576	27.998176128209217	22.796404082283527
49	22.266434779142084	25.384458220379525	30.26057446978153	22.08853253069686
50	21.859801068410142	28.40456067612821	28.114784324669113	21.620853930792535
51	21.256328735448893	29.635175809280817	26.636501355445702	22.471994099824588
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	249.0
1	431.5
2	614.0
3	2350.5
4	4087.0
5	3000.0
6	1913.0
7	1931.5
8	1950.0
9	2014.5
10	2079.0
11	2053.5
12	2028.0
13	1940.5
14	1853.0
15	1829.5
16	1806.0
17	1675.5
18	1545.0
19	1578.0
20	1611.0
21	1757.0
22	1903.0
23	1989.5
24	2076.0
25	2303.5
26	2921.5
27	3312.0
28	3814.0
29	4316.0
30	4998.0
31	5680.0
32	6603.5
33	7527.0
34	8524.0
35	9521.0
36	10901.0
37	12281.0
38	15192.0
39	18103.0
40	21957.5
41	25812.0
42	27759.5
43	29707.0
44	30355.0
45	31003.0
46	31768.0
47	32533.0
48	31709.0
49	30885.0
50	29649.5
51	28414.0
52	26159.0
53	23904.0
54	23059.0
55	22214.0
56	21199.0
57	20184.0
58	19206.0
59	18228.0
60	16588.5
61	14949.0
62	13143.5
63	11338.0
64	9864.5
65	8391.0
66	7047.5
67	5704.0
68	4815.0
69	3926.0
70	3439.0
71	2952.0
72	2515.0
73	2078.0
74	1568.5
75	774.5
76	490.0
77	381.5
78	273.0
79	231.5
80	190.0
81	134.0
82	78.0
83	51.5
84	25.0
85	19.0
86	13.0
87	8.0
88	3.0
89	4.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	401344.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.04642115265553
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.48612154865542	27.70063767045412
2	10.179928384288855	8.35699255530785
3	5.678373589224162	6.992307414162334
4	3.790499810152734	6.223458063463597
5	2.6930467739295882	5.52699660332571
6	2.0458661067120874	5.038528910282883
7	1.6239111629853438	4.6659019057276545
8	1.291516651043849	4.240970910753045
9	0.9631182670946197	3.557930220988338
>10	4.194943703086222	24.74913799102519
>50	0.03148331203461807	0.9108069711001378
>100	0.019374345867457267	1.3695654072940948
>500	6.054483083580396E-4	0.13618614465562764
>1k	0.0012108966167160792	0.5305792314594253
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1113	0.2773182108116728	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1022	0.2546443948333599	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	548	0.1365412214957742	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004983256258969861	0.0
2	0.0	0.0	0.0	0.02441795566895232	0.0
3	0.0	0.0	0.0	0.03288949130920108	0.0
4	0.0	0.0	0.0	0.05058005102854409	0.0
5	0.0	0.0	0.0	0.09094442672619997	0.0
6	0.0	0.0	0.0	0.14875019933025035	0.0
7	0.0	0.0	0.0	0.18438048158188486	0.0
8	0.0	0.0	0.0	0.4273142242066656	0.0
9	0.0	0.0	0.0	0.5254843725083719	0.0
10	0.0	0.0	0.0	0.6869418752989954	0.0
11	0.0	0.0	0.0	0.8389311911975762	0.0
12	0.0	0.0	0.0	0.9724824589379685	0.0
13	0.0	0.0	0.0	1.0325306968585553	0.0
14	0.0	0.0	0.0	1.054706187210971	0.0
15	0.0	0.0	0.0	1.0863498644554297	0.0
16	0.0	0.0	0.0	1.1606003827140807	0.0
17	0.0	0.0	0.0	1.2438207622388773	0.0
18	0.0	0.0	0.0	1.3751295646627333	0.0
19	0.0	0.0	0.0	1.4416560357199808	0.0
20	0.0	0.0	0.0	1.5012059480146707	0.0
21	0.0	0.0	0.0	1.5839280019135704	0.0
22	0.0	0.0	0.0	1.6758690798915643	0.0
23	0.0	0.0	0.0	1.7715476000637858	0.0
24	0.0	0.0	0.0	1.850033886142561	0.0
25	0.0	0.0	0.0	1.9113279381278903	0.0
26	0.0	0.0	0.0	1.9713761760484771	0.0
27	0.0	0.0	0.0	2.0376534842927763	0.0
28	0.0	0.0	0.0	2.1081665603572	0.0
29	0.0	0.0	0.0	2.201851778025833	0.0
30	0.0	0.0	0.0	2.299772763514591	0.0
31	0.0	0.0	0.0	2.387228910859512	0.0
32	0.0	0.0	0.0	2.4689543135066176	0.0
33	0.0	0.0	0.0	2.5643836708658907	0.0
34	0.0	0.0	0.0	2.665792935735927	0.0
35	0.0	0.0	0.0	2.8018358316058043	0.0
36	0.0	0.0	0.0	2.9015009567852017	0.0
37	0.0	0.0	0.0	3.0029102216552386	0.0
38	0.0	0.0	0.0	3.122508371870515	0.0
39	0.0	0.0	0.0	3.3198453197257214	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	130	0.0	45.000004	25
CACGACG	130	0.0	45.000004	26
GTACCGC	20	7.0277933E-4	45.0	31
GACGTCA	20	7.0277933E-4	45.0	9
CGCATCG	20	7.0277933E-4	45.0	21
ATAGCGG	20	7.0277933E-4	45.0	2
GGATCGT	20	7.0277933E-4	45.0	8
GCGTTAA	20	7.0277933E-4	45.0	1
ATGCGCG	40	6.7957444E-9	45.0	36
ATACGAA	20	7.0277933E-4	45.0	19
TGCGTAG	20	7.0277933E-4	45.0	2
GTTAATC	30	2.161958E-6	44.999996	40
CGAGACA	60	0.0	44.999996	22
CGAAGGA	140	0.0	43.39286	4
CGACGGT	135	0.0	43.333336	28
GACGGTC	135	0.0	43.333336	29
ACGGTCT	135	0.0	43.333336	30
ATAGGCG	120	0.0	43.124996	5
CGTTTTA	660	0.0	42.272728	1
CGTTATT	145	0.0	41.89655	1
>>END_MODULE