FastQCFastQC Report
Thu 26 May 2016
SRR1545780_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545780_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences548969
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25360.4619568682384616No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18760.3417315003215118No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10370.18889955534829836No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9800.1785164553918345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA207.8528846E-444.034
TCACGCC254.439791E-544.015
CAGGTAT207.8528846E-444.06
CGGGTCA207.8528846E-444.019
AGCCGTC207.8528846E-444.012
TCGTTAA207.8528846E-444.01
ACCGGTC207.8528846E-444.022
CGAACTG254.439791E-544.036
ACCGGCA207.8528846E-444.010
CATATCC207.8528846E-444.041
CTACTCG207.8528846E-444.023
CGACGTA254.439791E-544.023
GTTTCGA254.439791E-544.032
ACGTTAG302.525292E-644.02
ACGCACA207.8528846E-444.041
TAGACAT207.8528846E-444.024
CTATACG254.439791E-544.014
AGCTTGC254.439791E-544.033
GACAACG207.8528846E-444.040
ACCGAAT207.8528846E-444.015
Produced by FastQC (version 0.11.5)