##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1545780_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 548969 Sequences flagged as poor quality 0 Sequence length 50 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.633451069186055 31.0 31.0 33.0 30.0 34.0 2 31.81378912106148 31.0 31.0 34.0 30.0 34.0 3 31.827019376321797 31.0 31.0 34.0 30.0 34.0 4 35.7250045084513 37.0 35.0 37.0 35.0 37.0 5 27.298674424238893 35.0 28.0 37.0 0.0 37.0 6 31.473771743031026 36.0 29.0 37.0 17.0 37.0 7 14.822569216112385 0.0 0.0 32.0 0.0 37.0 8 25.028333111705763 17.0 17.0 35.0 17.0 37.0 9 33.335006166104094 32.0 32.0 37.0 28.0 39.0 10 36.24610679291545 37.0 35.0 39.0 33.0 39.0 11 37.21475711743286 39.0 37.0 39.0 34.0 39.0 12 36.938477400363226 39.0 35.0 39.0 33.0 39.0 13 36.26756519949214 39.0 35.0 39.0 32.0 39.0 14 37.57136195304289 40.0 35.0 41.0 33.0 41.0 15 37.92196098504652 40.0 35.0 41.0 33.0 41.0 16 38.04420650346376 40.0 35.0 41.0 34.0 41.0 17 38.08816709140225 40.0 35.0 41.0 34.0 41.0 18 38.06602012135476 40.0 36.0 41.0 34.0 41.0 19 37.966351469755125 40.0 36.0 41.0 34.0 41.0 20 37.9646555634289 40.0 35.0 41.0 34.0 41.0 21 37.7968245930098 40.0 35.0 41.0 34.0 41.0 22 37.695206833172726 39.0 35.0 41.0 33.0 41.0 23 37.59279667886529 39.0 35.0 41.0 33.0 41.0 24 37.44349498787727 39.0 35.0 41.0 33.0 41.0 25 37.27070198863688 39.0 35.0 41.0 33.0 41.0 26 37.0328251686343 38.0 35.0 40.0 33.0 41.0 27 36.779725266818346 38.0 35.0 40.0 33.0 41.0 28 36.855403492729096 38.0 35.0 40.0 33.0 41.0 29 36.8539935770508 38.0 35.0 40.0 33.0 41.0 30 36.64954669571506 38.0 35.0 40.0 32.0 41.0 31 36.41320548154814 38.0 35.0 40.0 32.0 41.0 32 36.095575524301005 38.0 35.0 40.0 31.0 41.0 33 35.83622572494986 38.0 35.0 40.0 30.0 41.0 34 35.57513083616743 38.0 35.0 40.0 30.0 41.0 35 35.39819916971632 38.0 35.0 40.0 29.0 41.0 36 35.17696991997727 38.0 34.0 40.0 27.0 41.0 37 34.9753228324368 38.0 34.0 40.0 27.0 41.0 38 34.83267179021038 37.0 34.0 40.0 27.0 41.0 39 34.80464834990683 37.0 34.0 40.0 26.0 41.0 40 34.55735387608408 37.0 34.0 40.0 25.0 40.0 41 34.40953860782667 37.0 34.0 40.0 24.0 40.0 42 34.397818456051255 37.0 34.0 40.0 24.0 40.0 43 34.273592862256336 36.0 33.0 40.0 23.0 41.0 44 34.02530561835003 36.0 33.0 40.0 23.0 40.0 45 33.78589683570475 36.0 33.0 39.0 23.0 40.0 46 33.7850807604801 36.0 33.0 39.0 23.0 40.0 47 33.64684344653341 35.0 33.0 39.0 23.0 40.0 48 33.664290697653236 35.0 33.0 39.0 23.0 40.0 49 33.57450056378411 35.0 33.0 39.0 23.0 40.0 50 33.55841404523753 35.0 33.0 39.0 23.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 10.0 15 14.0 16 26.0 17 72.0 18 186.0 19 359.0 20 717.0 21 1316.0 22 2163.0 23 3417.0 24 5377.0 25 7651.0 26 9945.0 27 10639.0 28 10256.0 29 11065.0 30 13264.0 31 18098.0 32 25949.0 33 53677.0 34 49627.0 35 57645.0 36 66210.0 37 129102.0 38 59758.0 39 12425.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.864822968145738 22.65191659274021 28.421459135215287 17.061801303898765 2 33.17855835211096 24.060010674555393 31.048201264552276 11.71322970878137 3 28.0973971207846 22.543713761614956 36.99735322030934 12.361535897291104 4 25.09886714914685 26.322251347526 33.51937905419067 15.059502449136472 5 17.06453369862415 47.663347110674735 24.96680140408657 10.305317786614545 6 19.944113419883454 39.35741362444874 29.862524113383454 10.835948842284354 7 38.14040501376216 56.33013886029994 4.636873848978722 0.8925822769591726 8 87.12368093644632 2.7378595148359928 8.884836848710947 1.25362270000674 9 83.9659434321428 3.963247469347085 10.073975033198595 1.9968340653115202 10 58.13898416850496 17.420109332220946 14.54198688814851 9.898919611125583 11 54.07627753115385 15.99398144521822 18.518714171474162 11.411026852153764 12 47.37717430310273 19.68380728237842 22.589435833353065 10.349582581165786 13 20.095852406966515 45.30929797493119 22.832072484967274 11.762777133135021 14 13.983121086983052 46.50444742781469 27.335969790643915 12.176461694558345 15 11.462213713342647 20.724849672750192 54.961209102881945 12.851727511025214 16 13.192001734159852 13.90242436276001 53.98993385783169 18.91564004524846 17 13.579455306219476 14.43706293069372 31.378274547378815 40.60520721570799 18 20.99845346458543 20.831413067040216 37.37278425557727 20.79734921279708 19 29.33480761208739 22.799101588614292 26.37762788062714 21.488462918671182 20 32.52187281977671 20.50516513682922 26.011669147073878 20.961292896320195 21 20.68823558343003 27.576784845774533 29.66907056682618 22.06590900396926 22 21.779007557803812 22.554825500164853 27.535434605597036 28.1307323364343 23 18.7589827476597 29.409857387211304 25.75919587444828 26.071963990680715 24 19.475234485007352 21.334902335104534 42.471797132442816 16.7180660474453 25 16.19708945313852 22.125293049334296 40.06929353023577 21.608323967291412 26 15.150764432964339 34.6766393002155 28.830771865077992 21.341824401742173 27 15.948988012073542 33.56382600839027 31.854804187485996 18.63238179205019 28 13.677275037388267 27.19461390351732 43.561476148926445 15.56663491016797 29 13.787117305348753 22.883441505804516 42.37470603986746 20.954735148979267 30 17.284764713490198 29.237352200215312 35.78744883590877 17.69043425038572 31 30.960400314043234 24.707223905174974 25.931883221092626 18.400492559689162 32 31.28336937058377 25.001411737274783 27.57532756858766 16.13989132355379 33 28.681947432368677 26.80497441567739 26.092912350242 18.420165801711935 34 18.577733897542483 27.80903839743228 29.855055567800733 23.758172137224506 35 19.771243913590748 24.827813592388644 32.79383717477672 22.607105319243892 36 32.85959680783432 22.70346777322581 26.97328993076112 17.46364548817875 37 20.56290974535903 31.767914035218748 30.065085642358675 17.60409057706355 38 20.284023323721375 33.21735835721143 23.42463782107915 23.073980497988046 39 20.929050638560646 30.186768287462495 28.170260980128205 20.71392009384865 40 23.655069776253303 25.485774242261407 25.916763970278833 24.94239201120646 41 17.231391936521007 23.130085669682625 28.49286571737202 31.14565667642435 42 23.54650262583133 24.27404826137724 24.06820785873155 28.111241254059884 43 23.17489694317894 25.958660689401402 25.21472068550319 25.651721681916467 44 19.827895564230403 31.40359473850072 27.998302272077296 20.770207425191586 45 17.250154380301986 38.70637504121362 22.3497137361126 21.69375684237179 46 23.22098333421377 32.55921554769031 25.879239082716875 18.340562035379048 47 22.28941889250577 27.019376321795946 27.612852456149618 23.07835232954866 48 23.99771207481661 23.103672520670564 30.999018159495346 21.89959724501748 49 21.54985072016817 21.872273297763627 32.834458776360776 23.743417205707427 50 19.861230779880103 32.48143337784101 27.102987600392737 20.554348241886153 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 476.0 1 619.0 2 762.0 3 4113.0 4 7464.0 5 5562.5 6 3661.0 7 3955.5 8 4250.0 9 4506.5 10 4763.0 11 4804.5 12 4846.0 13 4594.0 14 4342.0 15 3945.5 16 3549.0 17 3219.0 18 2889.0 19 2782.5 20 2676.0 21 2521.0 22 2366.0 23 2526.0 24 2686.0 25 2827.5 26 2969.0 27 3463.5 28 3958.0 29 5022.0 30 6086.0 31 7309.0 32 8532.0 33 8727.5 34 8923.0 35 9701.0 36 10479.0 37 14538.5 38 18598.0 39 27384.0 40 36170.0 41 46114.0 42 56058.0 43 58859.5 44 61661.0 45 57909.0 46 54157.0 47 49907.5 48 45658.0 49 42957.0 50 40256.0 51 36692.0 52 33128.0 53 29215.5 54 25303.0 55 23093.0 56 20883.0 57 19436.5 58 17990.0 59 16822.0 60 15654.0 61 13707.0 62 11760.0 63 10183.5 64 8607.0 65 7262.0 66 5917.0 67 5220.5 68 4524.0 69 3838.0 70 3152.0 71 2517.0 72 1882.0 73 1397.5 74 913.0 75 714.5 76 516.0 77 400.5 78 285.0 79 206.0 80 127.0 81 83.5 82 40.0 83 31.0 84 22.0 85 20.5 86 19.0 87 14.0 88 9.0 89 5.5 90 2.0 91 1.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 548969.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.30408739080587 #Duplication Level Percentage of deduplicated Percentage of total 1 71.75714872327303 16.722348647622084 2 6.182702285274419 2.8816446873474044 3 2.9760003099469734 2.0805891389420883 4 2.0646985220286425 1.9246365917209278 5 1.5392063737630024 1.7934899923329206 6 1.2785146024606346 1.7876769615698433 7 1.1739962673642623 1.915123812779566 8 0.9485425792546085 1.7683935328679838 9 0.845309341426067 1.7729246488371857 >10 10.364949271554392 52.71576983229588 >50 0.8114230964311612 11.75398263498649 >100 0.05435746956674769 1.7031378374479833 >500 7.87789414010836E-4 0.17991539082667063 >1k 0.002363368242032508 1.000366290422988 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2536 0.4619568682384616 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 1876 0.3417315003215118 No Hit CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1037 0.18889955534829836 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 980 0.1785164553918345 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.012204696440053992 0.0 2 0.0 0.0 0.0 0.044082634902881584 0.0 3 0.0 0.0 0.0 0.05738028923308967 0.0 4 0.0 0.0 0.0 0.07304601899196493 0.0 5 0.0 0.0 0.0 0.10692771358674169 0.0 6 0.0 0.0 0.0 0.16430800281983135 0.0 7 0.0 0.0 0.0 0.2018328903817884 0.0 8 0.0 0.0 0.0 0.3976545123677293 0.0 9 0.0 0.0 0.0 0.4725221278432844 0.0 10 0.0 0.0 0.0 0.6412019622237322 0.0 11 0.0 0.0 0.0 0.7960376633288947 0.0 12 0.0 0.0 0.0 0.9299250048727706 0.0 13 0.0 0.0 0.0 0.9780151520395505 0.0 14 0.0 0.0 0.0 0.9982348730074011 0.0 15 0.0 0.0 0.0 1.030841450063665 0.0 16 0.0 0.0 0.0 1.1027945111654756 0.0 17 0.0 0.0 0.0 1.194056494993342 0.0 18 0.0 0.0 0.0 1.3192001734159853 0.0 19 0.0 0.0 0.0 1.3685654381212782 0.0 20 0.0 0.0 0.0 1.4243062905191368 0.0 21 0.0 0.0 0.0 1.4995382252914098 0.0 22 0.0 0.0 0.0 1.563840581162142 0.0 23 0.0 0.0 0.0 1.648726977297443 0.0 24 0.0 0.0 0.0 1.7050143086403786 0.0 25 0.0 0.0 0.0 1.7520114979170045 0.0 26 0.0 0.0 0.0 1.8010124433255794 0.0 27 0.0 0.0 0.0 1.8542030606464117 0.0 28 0.0 0.0 0.0 1.9037504850000637 0.0 29 0.0 0.0 0.0 1.9598556566946403 0.0 30 0.0 0.0 0.0 2.0281655248292707 0.0 31 0.0 0.0 0.0 2.0952002754253884 0.0 32 0.0 0.0 0.0 2.15221624536176 0.0 33 0.0 0.0 0.0 2.212511088968594 0.0 34 0.0 0.0 0.0 2.2738988904655817 0.0 35 0.0 0.0 0.0 2.362428479568063 0.0 36 0.0 0.0 0.0 2.4272773143838724 0.0 37 0.0 0.0 0.0 2.4994125351340424 0.0 38 0.0 0.0 0.0 2.571729915532571 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGATCGA 20 7.8528846E-4 44.0 34 TCACGCC 25 4.439791E-5 44.0 15 CAGGTAT 20 7.8528846E-4 44.0 6 CGGGTCA 20 7.8528846E-4 44.0 19 AGCCGTC 20 7.8528846E-4 44.0 12 TCGTTAA 20 7.8528846E-4 44.0 1 ACCGGTC 20 7.8528846E-4 44.0 22 CGAACTG 25 4.439791E-5 44.0 36 ACCGGCA 20 7.8528846E-4 44.0 10 CATATCC 20 7.8528846E-4 44.0 41 CTACTCG 20 7.8528846E-4 44.0 23 CGACGTA 25 4.439791E-5 44.0 23 GTTTCGA 25 4.439791E-5 44.0 32 ACGTTAG 30 2.525292E-6 44.0 2 ACGCACA 20 7.8528846E-4 44.0 41 TAGACAT 20 7.8528846E-4 44.0 24 CTATACG 25 4.439791E-5 44.0 14 AGCTTGC 25 4.439791E-5 44.0 33 GACAACG 20 7.8528846E-4 44.0 40 ACCGAAT 20 7.8528846E-4 44.0 15 >>END_MODULE