FastQCFastQC Report
Thu 26 May 2016
SRR1545779_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545779_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences209704
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7590.3619387326898867No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6540.31186815702132525No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5630.2684736581085721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGGC750.045.0000044
AGGTCAA351.2055898E-745.07
GTTGATC551.8189894E-1245.016
TTTAGCG207.020086E-445.01
GTGCAAA253.8798673E-545.01
ATGGGCT453.8198777E-1045.05
TTAGGCG351.2055898E-745.05
TTAGGCC406.7684596E-945.05
AACCGGA207.020086E-445.024
GGTACTA351.2055898E-745.038
TCGTTCA207.020086E-445.016
CTCACGT406.7684596E-945.045
GAAGCGC207.020086E-445.09
ACACGCG207.020086E-445.036
ACCGGAA207.020086E-445.025
GAGCTTC207.020086E-445.010
CCGATGT351.2055898E-745.011
ACACCCA207.020086E-445.030
TATGGGC502.1827873E-1145.04
AGGACTA850.045.07