FastQCFastQC Report
Thu 26 May 2016
SRR1545778_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545778_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences907576
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36250.3994155861327316No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA20300.22367272823432968No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.17144569710966354No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14440.15910513279328672No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGGC650.044.00000417
GCATAAC207.8556064E-444.024
TTAGGCG1600.044.05
CGTATTA207.8556064E-444.024
CGTTGTC254.442098E-544.040
TCTTACG254.442098E-544.01
GCAGTCG207.8556064E-444.035
GTTACGA207.8556064E-444.01
CTAACGA207.8556064E-444.011
CCCGTAT207.8556064E-444.022
CCCCGTA207.8556064E-444.021
TAACCGT207.8556064E-444.029
TACGCAC302.5271256E-644.036
CGTAGGT254.442098E-544.04
GCTTAAC302.5271256E-644.040
TCTCGAG207.8556064E-444.019
GGTATCG254.442098E-544.024
CGGGATA254.442098E-544.06
ACTAACG207.8556064E-444.010
AGTTACG302.5271256E-644.01
Produced by FastQC (version 0.11.5)