##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545778_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	907576
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58643463467522	31.0	31.0	33.0	30.0	34.0
2	31.771820762117994	31.0	31.0	34.0	30.0	34.0
3	31.79840916903929	31.0	31.0	34.0	30.0	34.0
4	35.696752668647036	37.0	35.0	37.0	35.0	37.0
5	27.231836231896832	35.0	26.0	37.0	0.0	37.0
6	31.430847664548203	36.0	28.0	37.0	17.0	37.0
7	14.882417560623022	0.0	0.0	32.0	0.0	37.0
8	25.05361424277416	17.0	17.0	35.0	17.0	37.0
9	33.269882632418664	32.0	32.0	37.0	28.0	39.0
10	36.15347364848784	37.0	35.0	39.0	32.0	39.0
11	37.120772254885544	39.0	37.0	39.0	34.0	39.0
12	36.99686307262422	39.0	35.0	39.0	33.0	39.0
13	36.46652071011133	39.0	35.0	39.0	33.0	39.0
14	37.79318426225462	40.0	36.0	41.0	33.0	41.0
15	38.10157386268478	40.0	36.0	41.0	33.0	41.0
16	38.16180573307359	40.0	36.0	41.0	34.0	41.0
17	38.193134238895695	40.0	36.0	41.0	34.0	41.0
18	38.17374082170529	40.0	36.0	41.0	34.0	41.0
19	38.09714007421968	40.0	36.0	41.0	34.0	41.0
20	38.103287217819776	40.0	36.0	41.0	34.0	41.0
21	37.94673063192504	40.0	35.0	41.0	34.0	41.0
22	37.84386762100364	40.0	35.0	41.0	34.0	41.0
23	37.744981136565976	39.0	35.0	41.0	34.0	41.0
24	37.61349022010278	39.0	35.0	41.0	33.0	41.0
25	37.42797627967244	39.0	35.0	41.0	33.0	41.0
26	37.1740339101078	39.0	35.0	40.0	33.0	41.0
27	36.95798037850274	39.0	35.0	40.0	33.0	41.0
28	36.99952510864104	39.0	35.0	40.0	33.0	41.0
29	36.95568525390711	38.0	35.0	40.0	33.0	41.0
30	36.74790320590232	38.0	35.0	40.0	32.0	41.0
31	36.5235164878754	38.0	35.0	40.0	31.0	41.0
32	36.260034421359755	38.0	35.0	40.0	31.0	41.0
33	36.051070103220006	38.0	35.0	40.0	30.0	41.0
34	35.82094722645817	38.0	35.0	40.0	30.0	41.0
35	35.623715259107776	38.0	35.0	40.0	29.0	41.0
36	35.39835451796874	38.0	35.0	40.0	28.0	41.0
37	35.2142564369265	38.0	34.0	40.0	27.0	41.0
38	35.042280756652886	38.0	34.0	40.0	27.0	41.0
39	34.959603383077564	38.0	34.0	40.0	26.0	41.0
40	34.69335791162393	37.0	34.0	40.0	25.0	41.0
41	34.52081037841459	37.0	34.0	40.0	24.0	40.0
42	34.45984468518339	37.0	34.0	40.0	24.0	41.0
43	34.29792546299153	37.0	33.0	40.0	23.0	41.0
44	34.01960056237714	36.0	33.0	40.0	23.0	40.0
45	33.75142467407688	36.0	33.0	39.0	23.0	40.0
46	33.664003896092446	36.0	33.0	39.0	23.0	40.0
47	33.53407868872689	36.0	33.0	39.0	22.0	40.0
48	33.45149717489224	36.0	33.0	39.0	22.0	40.0
49	33.348677135578725	36.0	33.0	39.0	21.0	40.0
50	33.36565202253034	36.0	33.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	5.0
14	2.0
15	16.0
16	44.0
17	113.0
18	275.0
19	618.0
20	1185.0
21	2102.0
22	3322.0
23	5334.0
24	8387.0
25	12242.0
26	15341.0
27	16763.0
28	17218.0
29	19103.0
30	23137.0
31	30857.0
32	42613.0
33	79956.0
34	78979.0
35	94576.0
36	116617.0
37	217613.0
38	98665.0
39	22491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.51628513755322	23.229900305869702	27.757124472220507	16.496690084356572
2	33.35037506500833	24.986447415973977	29.839153966169224	11.824023552848468
3	29.593224148721433	23.829299144093717	33.81413787936217	12.763338827822684
4	26.51447371900535	25.888740997999065	33.06918649237089	14.527598790624696
5	17.02369829083184	48.00666831207524	24.235105379604573	10.734528017488342
6	20.751209816037445	39.99532821493737	29.125935458848623	10.127526510176558
7	38.61065078847391	56.22768781898155	4.242840269024302	0.9188211235202342
8	87.46661436617981	2.7315618747080133	8.290435181185927	1.5113885779262564
9	83.58760037726867	4.177170837483582	9.771413082761113	2.463815702486624
10	52.92967200542985	21.194478478937302	14.68262712984918	11.19322238578367
11	47.81748305376078	19.130408913413312	20.11148377656527	12.940624256260632
12	41.91197211032465	20.842441845090658	24.053743157597822	13.191842886986874
13	21.172772307773673	39.75777235184712	24.97653089107689	14.092924449302318
14	15.969461510661368	41.282162595749554	28.676386330180613	14.071989563408463
15	14.969324882985005	21.514672049503293	48.78037762126808	14.735625446243619
16	16.124379666275882	16.340890459862315	47.995319400248576	19.539410473613227
17	16.3426533976218	16.540102426683827	30.407481026382367	36.70976314931201
18	21.708485019436388	22.085423149135718	35.708634869145946	20.497456962281944
19	29.07899724100241	23.257997126411453	26.68382592752563	20.979179705060513
20	31.0163556550636	22.21984715329625	25.860093259407474	20.903703932232673
21	22.764154186536445	26.972947720080743	29.30432272338625	20.958575369996563
22	22.89990039401659	22.769112448984988	26.44241363808651	27.88857351891191
23	19.96086278173949	28.67153825134204	26.425886096591363	24.941712870327112
24	20.465613899001294	22.703222650224333	39.42325491198533	17.407908538789037
25	18.702786323128862	22.95697550397983	36.956243884809645	21.38399428808166
26	17.015985438134106	32.34550054210336	28.06464692764022	22.573867092122313
27	17.91188837078107	31.590852997434926	30.8150501996527	19.682208432131304
28	16.024773682865128	26.87554540886934	40.11432651370244	16.9853543945631
29	16.71242959267323	23.788090473965816	38.69593290258887	20.80354703077208
30	19.829854469487955	27.438583655804035	33.51741341771929	19.214148456988724
31	30.31668973176902	24.449412500991652	25.74825689529031	19.485640871949016
32	30.108663076150094	24.69985984644812	28.135274621629485	17.056202455772297
33	28.608292859220608	25.270831313300484	26.12717832996906	19.99369749750985
34	19.780161661392544	26.729772492882137	29.40271668708736	24.087349158637956
35	20.569186492370886	23.69751954657241	32.65963401412113	23.073659946935575
36	30.655283965199608	23.90984336298007	26.1464604617134	19.288412210106923
37	21.367136195756608	31.0984424444895	28.469902245101235	19.064519114652654
38	22.321877176126296	31.52639558560385	23.355289254012888	22.796437984256965
39	21.73790404329775	29.4478919671741	26.66751875325042	22.146685236277733
40	23.349780073514502	25.098944881750953	25.9860331256005	25.565241919134046
41	17.991771488007615	23.234528017488344	27.768473384047176	31.005227110456868
42	22.2925683358749	25.18059093673698	24.405339057004593	28.121501670383527
43	22.61331282449073	25.39666099588354	25.671018184702987	26.31900799492274
44	20.028956252699498	28.946776909041226	28.461969025183564	22.56229781307571
45	18.883377259865842	34.793559988364606	23.937609632691917	22.38545311907763
46	22.747185910601424	30.16606873694324	27.0657223196735	20.02102303278183
47	23.23750297495747	26.73109469620175	26.920830872566043	23.110571456274737
48	22.749169215580846	24.191031935617513	30.248926811638917	22.810872037162728
49	21.86659849973997	23.65851454864386	31.387674420654577	23.087212530961594
50	20.09727009087944	30.51050270170212	27.564192971167152	21.82803423625129
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	583.0
1	937.0
2	1291.0
3	6511.5
4	11732.0
5	8711.5
6	5691.0
7	5787.0
8	5883.0
9	6159.5
10	6436.0
11	6568.0
12	6700.0
13	6330.5
14	5961.0
15	5549.0
16	5137.0
17	4761.0
18	4385.0
19	4064.0
20	3743.0
21	3791.5
22	3840.0
23	4244.0
24	4648.0
25	5021.0
26	5394.0
27	6730.0
28	8066.0
29	9494.0
30	10922.0
31	11896.0
32	12870.0
33	15579.0
34	18288.0
35	19287.5
36	20287.0
37	24013.0
38	27739.0
39	38243.5
40	48748.0
41	64394.5
42	80041.0
43	85138.0
44	90235.0
45	86418.5
46	82602.0
47	81044.0
48	79486.0
49	76066.0
50	72646.0
51	65508.5
52	58371.0
53	52398.5
54	46426.0
55	42937.0
56	39448.0
57	37698.5
58	35949.0
59	32901.5
60	29854.0
61	25967.5
62	22081.0
63	19415.0
64	16749.0
65	14218.5
66	11688.0
67	9967.0
68	8246.0
69	7330.5
70	6415.0
71	5364.0
72	4313.0
73	3271.5
74	2230.0
75	1745.0
76	1260.0
77	933.0
78	606.0
79	480.0
80	354.0
81	255.5
82	157.0
83	100.0
84	43.0
85	29.5
86	16.0
87	12.0
88	8.0
89	6.5
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	907576.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.12549055688629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.47113949022987	17.96652772532103
2	5.883877977969277	2.83902885190738
3	2.6944497350434946	1.9501476491638983
4	1.7286459989252785	1.6681773089308432
5	1.3892090139037736	1.6757674473238404
6	1.1146607382704372	1.6135042269165203
7	0.9884707790336839	1.6693139711734626
8	0.8285182579489012	1.599072752668328
9	0.7971985966589616	1.7309526494092908
>10	9.00923599948012	47.60070848258869
>50	1.0100632929847637	15.725197613403088
>100	0.0826198343638073	2.964403222225223
>500	0.0	0.0
>1k	0.0019102851876024811	0.9971980989684522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3625	0.3994155861327316	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2030	0.22367272823432968	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1556	0.17144569710966354	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1444	0.15910513279328672	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007602669087767857	0.0
2	0.0	0.0	0.0	0.03669114211922748	0.0
3	0.0	0.0	0.0	0.05046409336518374	0.0
4	0.0	0.0	0.0	0.07635724170758151	0.0
5	0.0	0.0	0.0	0.12131215457438276	0.0
6	0.0	0.0	0.0	0.21298491806746764	0.0
7	0.0	0.0	0.0	0.2643304803123926	0.0
8	0.0	0.0	0.0	0.574056607931457	0.0
9	0.0	0.0	0.0	0.7171851172794345	0.0
10	0.0	0.0	0.0	0.9581566722786852	0.0
11	0.0	0.0	0.0	1.1764304036246	0.0
12	0.0	0.0	0.0	1.3714553932673408	0.0
13	0.0	0.0	0.0	1.451007959663984	0.0
14	0.0	0.0	0.0	1.4770112916163496	0.0
15	0.0	0.0	0.0	1.5200930831137007	0.0
16	0.0	0.0	0.0	1.6239962273131947	0.0
17	0.0	0.0	0.0	1.7392482833393568	0.0
18	0.0	0.0	0.0	1.9332816204923886	0.0
19	0.0	0.0	0.0	2.00401949809162	0.0
20	0.0	0.0	0.0	2.0909543663560957	0.0
21	0.0	0.0	0.0	2.188246493957531	0.0
22	0.0	0.0	0.0	2.293912575916507	0.0
23	0.0	0.0	0.0	2.4135719763413754	0.0
24	0.0	0.0	0.0	2.5046938217846217	0.0
25	0.0	0.0	0.0	2.5700327024954386	0.0
26	0.0	0.0	0.0	2.6424233342441843	0.0
27	0.0	0.0	0.0	2.7075418477350657	0.0
28	0.0	0.0	0.0	2.7831278041728735	0.0
29	0.0	0.0	0.0	2.861468350859873	0.0
30	0.0	0.0	0.0	2.960303049000855	0.0
31	0.0	0.0	0.0	3.0564933405026133	0.0
32	0.0	0.0	0.0	3.1406736185178983	0.0
33	0.0	0.0	0.0	3.229591791761792	0.0
34	0.0	0.0	0.0	3.3197219847153296	0.0
35	0.0	0.0	0.0	3.4391610179202625	0.0
36	0.0	0.0	0.0	3.535351309422021	0.0
37	0.0	0.0	0.0	3.6308804992639736	0.0
38	0.0	0.0	0.0	3.7279522596454733	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGGC	65	0.0	44.000004	17
GCATAAC	20	7.8556064E-4	44.0	24
TTAGGCG	160	0.0	44.0	5
CGTATTA	20	7.8556064E-4	44.0	24
CGTTGTC	25	4.442098E-5	44.0	40
TCTTACG	25	4.442098E-5	44.0	1
GCAGTCG	20	7.8556064E-4	44.0	35
GTTACGA	20	7.8556064E-4	44.0	1
CTAACGA	20	7.8556064E-4	44.0	11
CCCGTAT	20	7.8556064E-4	44.0	22
CCCCGTA	20	7.8556064E-4	44.0	21
TAACCGT	20	7.8556064E-4	44.0	29
TACGCAC	30	2.5271256E-6	44.0	36
CGTAGGT	25	4.442098E-5	44.0	4
GCTTAAC	30	2.5271256E-6	44.0	40
TCTCGAG	20	7.8556064E-4	44.0	19
GGTATCG	25	4.442098E-5	44.0	24
CGGGATA	25	4.442098E-5	44.0	6
ACTAACG	20	7.8556064E-4	44.0	10
AGTTACG	30	2.5271256E-6	44.0	1
>>END_MODULE