##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545777_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	343920
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081975459409165	31.0	31.0	33.0	30.0	33.0
2	31.233504884856945	31.0	31.0	33.0	30.0	34.0
3	31.146359618515934	31.0	31.0	34.0	28.0	34.0
4	35.11359618515934	35.0	35.0	37.0	33.0	37.0
5	32.90866480576879	35.0	35.0	37.0	28.0	37.0
6	34.08394684810421	35.0	35.0	37.0	29.0	37.0
7	15.022926843451966	0.0	0.0	35.0	0.0	37.0
8	24.782705280297744	17.0	17.0	35.0	17.0	37.0
9	32.75653058850896	32.0	32.0	37.0	27.0	39.0
10	35.62296173528728	37.0	35.0	37.0	32.0	39.0
11	36.58466794603396	37.0	35.0	39.0	33.0	39.0
12	36.36338683414748	37.0	35.0	39.0	32.0	39.0
13	35.949685973482204	37.0	35.0	39.0	31.0	39.0
14	37.00422772737846	39.0	35.0	40.0	31.0	41.0
15	37.40682135380321	39.0	35.0	41.0	32.0	41.0
16	37.47276110723424	39.0	35.0	41.0	33.0	41.0
17	37.43018434519656	39.0	35.0	41.0	32.0	41.0
18	37.3533350779251	39.0	35.0	41.0	32.0	41.0
19	37.27812863456618	39.0	35.0	41.0	32.0	41.0
20	37.35551872528495	39.0	35.0	41.0	32.0	41.0
21	37.197999534775526	39.0	35.0	41.0	32.0	41.0
22	37.23663061177018	39.0	35.0	41.0	32.0	41.0
23	37.230992672714585	39.0	35.0	41.0	32.0	41.0
24	37.07692195859502	39.0	35.0	41.0	32.0	41.0
25	36.80893521749244	38.0	35.0	40.0	32.0	41.0
26	36.68214410327983	38.0	35.0	40.0	31.0	41.0
27	36.570321586415446	38.0	35.0	40.0	31.0	41.0
28	36.488273435682714	38.0	35.0	40.0	31.0	41.0
29	36.49180041870202	38.0	35.0	40.0	31.0	41.0
30	36.25708595022098	38.0	35.0	40.0	30.0	41.0
31	36.108388578739245	38.0	35.0	40.0	30.0	41.0
32	35.95531809723192	38.0	35.0	40.0	30.0	41.0
33	35.77398232147011	38.0	34.0	40.0	29.0	41.0
34	35.59593800883926	38.0	34.0	40.0	29.0	41.0
35	35.38417946033962	38.0	34.0	40.0	27.0	41.0
36	35.17537508722959	38.0	34.0	40.0	26.0	41.0
37	34.99175389625494	38.0	34.0	40.0	25.0	41.0
38	34.9166346824843	38.0	34.0	40.0	25.0	41.0
39	34.785679809257964	38.0	34.0	40.0	24.0	41.0
40	34.58178064666201	37.0	33.0	40.0	23.0	41.0
41	34.46208711328216	37.0	33.0	40.0	23.0	41.0
42	34.373944521981855	37.0	33.0	40.0	23.0	41.0
43	34.261226448011165	37.0	33.0	40.0	23.0	41.0
44	33.983699697604095	37.0	33.0	40.0	22.0	41.0
45	33.83111479413817	36.0	33.0	40.0	22.0	41.0
46	33.77619504535939	36.0	33.0	40.0	21.0	41.0
47	33.752922191207254	36.0	33.0	40.0	21.0	41.0
48	33.70776634100954	36.0	33.0	40.0	21.0	41.0
49	33.621156082809954	36.0	33.0	40.0	20.0	41.0
50	33.442716329378925	36.0	33.0	40.0	20.0	41.0
51	33.26477669225401	35.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	10.0
15	11.0
16	31.0
17	67.0
18	209.0
19	379.0
20	731.0
21	1125.0
22	1849.0
23	2595.0
24	3863.0
25	5225.0
26	6278.0
27	6785.0
28	7300.0
29	8405.0
30	10031.0
31	13059.0
32	17311.0
33	26514.0
34	33960.0
35	35060.0
36	39006.0
37	60235.0
38	54037.0
39	9844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.95190742033031	24.021574784833682	28.09868574086997	15.927832053966037
2	32.828855547801815	24.871481739939522	30.47540125610607	11.824261456152593
3	30.07676203768318	23.757850662944872	33.28739241684113	12.877994882530821
4	26.97575017445918	26.636717841358454	31.641660851360783	14.745871132821586
5	20.828390323331007	36.695161665503605	29.213479879041635	13.262968132123751
6	20.849034659223076	40.60740869969761	28.537450569899974	10.006106071179344
7	37.174342870434984	57.723889276575946	4.4062572691323565	0.6955105838567108
8	88.18998604326588	2.925389625494301	7.476157245871133	1.4084670853686905
9	84.10415212840195	4.378634566178181	8.978250755989766	2.5389625494301002
10	53.24406838799721	21.694870900209352	14.000348918353106	11.060711793440335
11	48.48336822516865	19.271051407304025	19.46179344033496	12.78378692719237
12	42.459002093510115	21.30786229355664	23.210921144452197	13.022214468481042
13	21.28169341707374	40.64549895324494	23.999767387764596	14.073040241916726
14	15.861246801581764	42.4482437776227	27.81111886485229	13.879390555943244
15	14.91044428936962	21.86991160735055	48.528146080483836	14.691498022795999
16	16.172947197022562	16.575075598976508	47.71807397069086	19.53390323331007
17	16.265410560595488	16.811467783205398	29.774656896952777	37.14846475924634
18	21.55384973249593	22.382821586415446	35.63328681088625	20.430041870202373
19	29.730460572226097	23.269946499185856	26.161025819958127	20.838567108629913
20	31.603570597813444	22.386892300535006	25.291346824842986	20.71819027680856
21	22.811409630146546	27.583158874156783	28.643870667597117	20.961560828099557
22	22.85793207722726	23.08269364968597	25.71237497092347	28.346999302163294
23	19.885729239357993	29.3216445685043	25.717317980925795	25.07530821121191
24	20.236101418934634	23.000407071411956	39.63770644335892	17.125785066294487
25	18.654047452896023	23.146371249127704	37.06210746685276	21.13747383112352
26	16.90654803442661	33.06117701791114	27.581414282391254	22.450860665270994
27	17.900383810188416	32.740462898348454	29.893579902302864	19.46557338916027
28	15.903117003954408	27.49273086764364	39.74209118399628	16.862060944405677
29	16.747208653175157	24.311467783205394	38.13444987206327	20.806873691556177
30	19.613282158641546	28.413293789253313	32.78087927424983	19.192544777855318
31	30.88305419865085	24.666492207490116	25.27012095836241	19.180332635496626
32	30.66759711560828	24.726971388695045	27.79105605954873	16.81437543614794
33	29.51238660153524	25.298325191905096	25.333507792509884	19.85578041404978
34	19.856652709932543	26.773086764363807	28.781984182367992	24.58827634333566
35	20.696382879739474	23.64009071877181	32.680274482437774	22.983251919050943
36	31.35002326122354	24.195161665503605	25.505350081414278	18.949464991858573
37	21.158699697604096	32.04902302861131	28.02454059083508	18.767736682949522
38	21.98883461270063	32.439230053500815	22.95533845080251	22.616596882996046
39	21.83705512909979	30.170969993021636	26.251744591765526	21.74023028611305
40	23.845661781809724	25.270993254245173	25.3864270760642	25.496917887880905
41	17.94923237962317	23.28913700860665	27.431379390555943	31.330251221214233
42	21.93824145150035	25.183472900674573	24.041637590137242	28.836648057687835
43	22.520353570597816	25.219237031867873	25.40910676901605	26.85130262851826
44	19.862758781111886	28.89421958595022	28.758722958827633	22.48429867411026
45	18.719760409397534	35.73679925564085	23.463305419865087	22.080134915096533
46	22.554373110025587	30.906606187485465	26.515177948360087	20.023842754128868
47	22.97452896022331	27.08246103745057	26.53960223307746	23.403407769248663
48	22.464235868806696	24.57984414980228	30.51756222377297	22.438357757618054
49	22.01616655036055	23.9480111653873	31.505001163061173	22.530821121190975
50	20.137822749476623	31.49627820423354	27.087404047452896	21.27849499883694
51	18.95906024656897	32.70498953244941	24.90899046289835	23.426959758083274
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	259.0
1	390.0
2	521.0
3	2054.5
4	3588.0
5	2740.0
6	1892.0
7	1936.0
8	1980.0
9	2080.5
10	2181.0
11	2123.5
12	2066.0
13	2010.5
14	1955.0
15	1858.0
16	1761.0
17	1599.0
18	1437.0
19	1356.5
20	1276.0
21	1349.0
22	1422.0
23	1499.5
24	1577.0
25	1801.5
26	2425.5
27	2825.0
28	3310.5
29	3796.0
30	4290.5
31	4785.0
32	5576.0
33	6367.0
34	7061.5
35	7756.0
36	8778.5
37	9801.0
38	12919.0
39	16037.0
40	21611.0
41	27185.0
42	30248.5
43	33312.0
44	32020.5
45	30729.0
46	30274.5
47	29820.0
48	28559.0
49	27298.0
50	25918.5
51	24539.0
52	22283.5
53	20028.0
54	18090.5
55	16153.0
56	15237.0
57	14321.0
58	13476.5
59	12632.0
60	10972.0
61	9312.0
62	8138.5
63	6965.0
64	6002.0
65	5039.0
66	4258.0
67	3477.0
68	3025.0
69	2573.0
70	2375.5
71	2178.0
72	1847.0
73	1516.0
74	1111.0
75	544.0
76	382.0
77	305.0
78	228.0
79	175.5
80	123.0
81	93.5
82	64.0
83	39.0
84	14.0
85	13.5
86	13.0
87	7.5
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	343920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.16366848444319
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.38083669493122	20.02289551785867
2	8.282619013535635	4.996683482144696
3	4.61377127059438	4.175048012077713
4	3.2061925652568197	3.8684211854277275
5	2.4064144376113825	3.629314366614377
6	1.9032590850765352	3.444556564935196
7	1.548941789450184	3.2705236647072886
8	1.4142059607849295	3.4126111815872067
9	1.1507262939683853	3.1239113802834866
>10	8.985245867455225	46.92700565692342
>50	0.0908205457547321	1.775243788621791
>100	0.01397239165457417	0.5924503605236028
>500	0.002994083925980179	0.7613348382948126
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	954	0.2773900907187718	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	800	0.23261223540358222	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	775	0.22534310304722027	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007559897650616422	0.0
2	0.0	0.0	0.0	0.035182600604791815	0.0
3	0.0	0.0	0.0	0.051756222377297045	0.0
4	0.0	0.0	0.0	0.07472668062340079	0.0
5	0.0	0.0	0.0	0.1218306582926262	0.0
6	0.0	0.0	0.0	0.1753314724354501	0.0
7	0.0	0.0	0.0	0.20469876715515237	0.0
8	0.0	0.0	0.0	0.458246103745057	0.0
9	0.0	0.0	0.0	0.5800767620376832	0.0
10	0.0	0.0	0.0	0.7818678762502907	0.0
11	0.0	0.0	0.0	0.9513840428006514	0.0
12	0.0	0.0	0.0	1.11363107699465	0.0
13	0.0	0.0	0.0	1.1752733193765992	0.0
14	0.0	0.0	0.0	1.1996976040939753	0.0
15	0.0	0.0	0.0	1.2372063270528029	0.0
16	0.0	0.0	0.0	1.3200744359153291	0.0
17	0.0	0.0	0.0	1.4145731565480344	0.0
18	0.0	0.0	0.0	1.5776924866247966	0.0
19	0.0	0.0	0.0	1.6343917190044197	0.0
20	0.0	0.0	0.0	1.700395440800186	0.0
21	0.0	0.0	0.0	1.780646662014422	0.0
22	0.0	0.0	0.0	1.8667131891137474	0.0
23	0.0	0.0	0.0	1.9684810421028147	0.0
24	0.0	0.0	0.0	2.033030937427309	0.0
25	0.0	0.0	0.0	2.094091649220749	0.0
26	0.0	0.0	0.0	2.1388695045359385	0.0
27	0.0	0.0	0.0	2.197604093975343	0.0
28	0.0	0.0	0.0	2.2676785298906723	0.0
29	0.0	0.0	0.0	2.328448476389858	0.0
30	0.0	0.0	0.0	2.4098627587811117	0.0
31	0.0	0.0	0.0	2.49738311235171	0.0
32	0.0	0.0	0.0	2.564549895324494	0.0
33	0.0	0.0	0.0	2.6401488718306583	0.0
34	0.0	0.0	0.0	2.7241800418702025	0.0
35	0.0	0.0	0.0	2.8253663642707605	0.0
36	0.0	0.0	0.0	2.9067806466620145	0.0
37	0.0	0.0	0.0	2.9992440102349383	0.0
38	0.0	0.0	0.0	3.0803675273319375	0.0
39	0.0	0.0	0.0	3.173121656199116	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCG	20	7.026384E-4	45.000004	35
AGGTAGT	20	7.026384E-4	45.000004	7
AGGTAAT	30	2.1610485E-6	45.000004	7
CGACGCT	20	7.026384E-4	45.000004	12
CCTAGGA	20	7.026384E-4	45.000004	4
CCCTAGA	30	2.1610485E-6	45.000004	37
CGACCGT	20	7.026384E-4	45.000004	26
CGATTGT	20	7.026384E-4	45.000004	10
CGCGAGG	30	2.1610485E-6	45.000004	3
GCGTTAT	20	7.026384E-4	45.000004	44
AGACGAG	20	7.026384E-4	45.000004	2
ATATCGA	20	7.026384E-4	45.000004	10
TTAACGT	20	7.026384E-4	45.000004	15
GTGAGTC	20	7.026384E-4	45.000004	18
ATACGCA	20	7.026384E-4	45.000004	25
CGACTAG	20	7.026384E-4	45.000004	2
TAGTGCA	30	2.1610485E-6	45.000004	1
GCGTATA	20	7.026384E-4	45.000004	1
AATACGC	20	7.026384E-4	45.000004	24
TCAACGT	20	7.026384E-4	45.000004	33
>>END_MODULE