FastQCFastQC Report
Thu 26 May 2016
SRR1545776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545776_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1074983
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34150.31767944237257706No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19750.18372383563274955No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14760.1373044969083232No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14180.13190906274796904No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACCGA254.442647E-544.040
TCGATAT254.442647E-544.016
TCATACG254.442647E-544.034
CTCGATA454.802132E-1043.99999624
ACGGTCT2300.043.0434830
CGTTTTA21300.042.7605631
CGTTATT11150.042.618831
CACGACG2350.042.1276626
CGGTCTA2350.042.1276631
TTACGGG3500.041.4857183
AGGCGAT9850.041.3197947
GTTTTAT24800.041.0725822
ACGGGTA603.6379788E-1240.3333325
AGCGTAA501.3478711E-939.6000021
CGCATGG501.3478711E-939.6000022
CGTTCGA501.3478711E-939.60000214
TCGATAC501.3478711E-939.60000225
CCAGTCG501.3478711E-939.60000228
ATAGGCC1450.039.4482735
AAGGCGA6050.039.2727286
Produced by FastQC (version 0.11.5)