##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545776_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1074983
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.477264291621356	31.0	31.0	33.0	30.0	34.0
2	31.662383498157645	31.0	31.0	34.0	30.0	34.0
3	31.731222726312883	31.0	31.0	34.0	30.0	34.0
4	35.6405385015391	37.0	35.0	37.0	33.0	37.0
5	27.07938265070238	35.0	25.0	37.0	0.0	37.0
6	31.311203060885614	35.0	26.0	37.0	17.0	37.0
7	15.002678181887527	0.0	0.0	32.0	0.0	37.0
8	25.080548250530473	17.0	17.0	35.0	17.0	37.0
9	33.106331914086084	32.0	32.0	37.0	28.0	39.0
10	35.946907067367576	37.0	35.0	39.0	32.0	39.0
11	36.95433788255256	38.0	37.0	39.0	34.0	39.0
12	36.96654923845308	39.0	35.0	39.0	33.0	39.0
13	36.50017535161021	39.0	35.0	39.0	32.0	39.0
14	37.82205765114425	40.0	36.0	41.0	33.0	41.0
15	38.062930297502376	40.0	36.0	41.0	33.0	41.0
16	38.12618897229073	40.0	36.0	41.0	33.0	41.0
17	38.127748066713615	40.0	36.0	41.0	34.0	41.0
18	38.12658060639099	40.0	36.0	41.0	34.0	41.0
19	38.087607897055115	40.0	36.0	41.0	34.0	41.0
20	38.11467995307833	40.0	36.0	41.0	34.0	41.0
21	37.97312422615055	40.0	36.0	41.0	34.0	41.0
22	37.90153239632627	40.0	36.0	41.0	33.0	41.0
23	37.81473939587882	40.0	35.0	41.0	33.0	41.0
24	37.67247854152112	40.0	35.0	41.0	33.0	41.0
25	37.51250484891389	39.0	35.0	41.0	33.0	41.0
26	37.25585706936761	39.0	35.0	40.0	33.0	41.0
27	37.07040390406174	39.0	35.0	40.0	32.0	41.0
28	37.0815715225264	39.0	35.0	40.0	32.0	41.0
29	37.01058063243791	39.0	35.0	40.0	32.0	41.0
30	36.814904049645435	38.0	35.0	40.0	32.0	41.0
31	36.66094161489065	38.0	35.0	40.0	31.0	41.0
32	36.45670303623406	38.0	35.0	40.0	31.0	41.0
33	36.25721616062766	38.0	35.0	40.0	30.0	41.0
34	36.03957271882439	38.0	35.0	40.0	30.0	41.0
35	35.90220310460724	38.0	35.0	40.0	30.0	41.0
36	35.740575432355676	38.0	35.0	40.0	29.0	41.0
37	35.647062325636774	38.0	35.0	40.0	29.0	41.0
38	35.447111256643126	38.0	34.0	40.0	29.0	41.0
39	35.387369846778974	38.0	34.0	40.0	28.0	41.0
40	35.146295336763465	38.0	34.0	40.0	27.0	41.0
41	34.95849050636149	37.0	34.0	40.0	27.0	40.0
42	34.95866353235353	38.0	34.0	40.0	27.0	41.0
43	34.81690966275746	37.0	34.0	40.0	26.0	41.0
44	34.603438379955776	37.0	34.0	40.0	26.0	40.0
45	34.406345030572574	37.0	33.0	40.0	25.0	40.0
46	34.36434064538695	37.0	33.0	40.0	25.0	40.0
47	34.17763071602062	36.0	33.0	39.0	25.0	40.0
48	34.106126329439626	36.0	33.0	39.0	24.0	40.0
49	33.958354690260215	36.0	33.0	39.0	24.0	40.0
50	34.05057847426424	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	2.0
12	3.0
13	6.0
14	11.0
15	21.0
16	61.0
17	138.0
18	371.0
19	801.0
20	1406.0
21	2425.0
22	3663.0
23	5654.0
24	8525.0
25	12099.0
26	15281.0
27	17203.0
28	18720.0
29	21980.0
30	26496.0
31	35932.0
32	49255.0
33	87955.0
34	90253.0
35	113301.0
36	149277.0
37	266153.0
38	117871.0
39	30119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.86790023656188	22.785662656990855	27.774020612418987	14.572416494028278
2	33.33931792409741	24.293221381175332	29.634422125745246	12.733038568982021
3	31.20291204605096	23.78744594100558	32.07250719313701	12.937134819806454
4	28.17914329808006	26.470279064878234	30.341782149113055	15.008795487928648
5	17.666604960264486	48.55025614358552	22.5736593043797	11.209479591770288
6	20.05668926857448	41.83256851503698	28.03979225717988	10.070949959208656
7	39.31624965232008	55.77464945957284	3.701360858729859	1.2077400293772087
8	86.77262803225726	3.413821427873743	7.603096979208043	2.210453560660959
9	82.20734653478242	5.149848881331147	9.30703090188403	3.3357736820024133
10	47.772569426679304	24.414990748690908	14.967027385549352	12.84541243908043
11	43.033703788804104	21.493270126132227	20.894749033240526	14.578277051823147
12	39.31997064139619	20.16413282814705	25.194258885954472	15.321637644502283
13	23.954890449430362	33.072243933159875	25.881525568311314	17.09134004909845
14	18.165496570643445	36.41220372787291	28.31579662190007	17.106503079583586
15	17.116642774815976	23.066039183875468	42.71100101117879	17.106317030129777
16	19.640124541504377	18.18707830728486	42.14410832543398	20.02868882577678
17	18.266428399332828	18.594061487483987	30.329130786254293	32.81037932692889
18	22.623055434365007	23.20157621097264	32.81930970071155	21.356058653950807
19	28.998877191546285	25.31714455019289	25.004209368892344	20.679768889368482
20	31.186539694116096	22.687428545381646	24.742716861568972	21.383314898933286
21	24.577877045497466	26.999775810408167	27.608901722166767	20.813445421927604
22	23.380555785533353	23.177575831431753	26.498651606583547	26.94321677645135
23	22.2692823979542	29.02883115360894	24.91825452123429	23.78363192720257
24	22.143419942454905	23.834888551725932	35.23627815509641	18.785413350722756
25	21.683877791555773	22.69868453733687	32.30069684822923	23.316740822878128
26	18.961509158749486	30.475830780579788	27.316338956057912	23.246321104612814
27	20.42720675582777	29.189856955877442	28.149375385471213	22.23356090282358
28	18.855740044261164	27.171685505724273	34.830504296347016	19.142070153667547
29	20.31362356427962	24.72299561946561	33.70881213935476	21.254568676900007
30	21.681645198110107	26.45651140529664	31.518824018612385	20.343019377980863
31	29.529118134891437	24.094334515057447	25.27398107691005	21.10256627314106
32	30.034056352519062	25.06597778755571	26.028039513183	18.871926346742228
33	28.277749508596877	24.802159662059772	25.438541818800854	21.48154901054249
34	21.23233576717027	26.56376891541541	28.62556896248592	23.578326354928404
35	21.812344939408344	24.297500518612853	30.229780377922253	23.660374164056545
36	28.647801872215656	25.749802555017148	25.158444366096955	20.443951206670246
37	22.322306492288714	30.484947203816247	26.700515263962316	20.492231039932722
38	22.304073645815794	31.20533068895043	23.32343860321512	23.167157062018656
39	22.541472748871378	28.581196167753355	25.73119760963662	23.146133473738654
40	24.220476044737453	24.82457862124331	26.72972502820975	24.225220305809486
41	19.808685346651995	24.10075322121373	27.735880474388896	28.354680957745376
42	22.86184990832413	26.207112112470615	23.732003203771594	27.199034775433656
43	22.569101092761468	25.737988414700514	24.81806689036013	26.874843602177894
44	21.97151024713879	27.401363556446938	27.70704280904907	22.920083387365196
45	20.74897928618406	31.440962322194864	24.397688149487013	23.412370242134063
46	22.68259125958271	29.545490486826303	26.53660569515983	21.235312558431158
47	22.90417615906484	26.682282417489393	26.334742037781062	24.078799385664702
48	22.758778510915985	24.730251548164016	29.309207680493554	23.201762260426445
49	22.201467372042163	24.12652107056577	30.150616335328095	23.521395222063976
50	20.41334607151927	28.86436343644504	27.537737806086238	23.184552685949452
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	565.0
1	1181.0
2	1797.0
3	6767.0
4	11737.0
5	8558.0
6	5379.0
7	5349.0
8	5319.0
9	5433.0
10	5547.0
11	5519.5
12	5492.0
13	5276.0
14	5060.0
15	4772.5
16	4485.0
17	4188.5
18	3892.0
19	3742.5
20	3593.0
21	3844.0
22	4095.0
23	4403.0
24	4711.0
25	5796.5
26	6882.0
27	8351.5
28	9821.0
29	11757.0
30	13693.0
31	15726.0
32	17759.0
33	19705.0
34	21651.0
35	22869.0
36	24087.0
37	28758.0
38	33429.0
39	45363.5
40	57298.0
41	68654.0
42	80010.0
43	83529.0
44	87048.0
45	88613.0
46	90178.0
47	88899.0
48	87620.0
49	84179.0
50	80738.0
51	74946.5
52	69155.0
53	65939.0
54	62723.0
55	60688.0
56	58653.0
57	57206.0
58	55759.0
59	52115.5
60	48472.0
61	42008.5
62	35545.0
63	30206.5
64	24868.0
65	20865.5
66	16863.0
67	14370.0
68	11877.0
69	10193.5
70	8510.0
71	6900.5
72	5291.0
73	3946.5
74	2602.0
75	1966.0
76	1330.0
77	1051.5
78	773.0
79	565.5
80	358.0
81	266.5
82	175.0
83	132.5
84	90.0
85	61.0
86	32.0
87	21.0
88	10.0
89	8.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1074983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.15868717957488
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.0996389898768	17.384562015678657
2	7.3226383489805364	3.6845593510232
3	3.685607321861508	2.781751250323933
4	2.499664765917039	2.5155313559804817
5	1.8993403741879848	2.3892455160866106
6	1.6242583881299222	2.451852521145674
7	1.338601457797987	2.357421873440372
8	1.1405012503655405	2.2954811348688513
9	1.0086833778597546	2.2839434610129445
>10	9.839820429542566	50.686776104710816
>50	0.47964014804839517	7.538374777913718
>100	0.05840488003292352	2.5538201337440656
>500	0.0016001336995320936	0.24295258336386366
>1k	0.0016001336995320936	0.8337279207068129
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3415	0.31767944237257706	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1975	0.18372383563274955	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1476	0.1373044969083232	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1418	0.13190906274796904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009581546870973774	0.0
2	0.0	0.0	0.0	0.03851223693770041	0.0
3	0.0	0.0	0.0	0.05153569870407253	0.0
4	0.0	0.0	0.0	0.07767564696371942	0.0
5	0.0	0.0	0.0	0.1280020242180574	0.0
6	0.0	0.0	0.0	0.23618978160584866	0.0
7	0.0	0.0	0.0	0.31256308239293085	0.0
8	0.0	0.0	0.0	0.7938730193872834	0.0
9	0.0	0.0	0.0	1.011829954520211	0.0
10	0.0	0.0	0.0	1.3469980455504877	0.0
11	0.0	0.0	0.0	1.5755597995503186	0.0
12	0.0	0.0	0.0	1.8004935892009455	0.0
13	0.0	0.0	0.0	1.8857972637706828	0.0
14	0.0	0.0	0.0	1.9171465967368786	0.0
15	0.0	0.0	0.0	1.964403158003429	0.0
16	0.0	0.0	0.0	2.0652419619659104	0.0
17	0.0	0.0	0.0	2.1837554640398964	0.0
18	0.0	0.0	0.0	2.370456090933531	0.0
19	0.0	0.0	0.0	2.4481317378972505	0.0
20	0.0	0.0	0.0	2.5250631870457485	0.0
21	0.0	0.0	0.0	2.632227672437611	0.0
22	0.0	0.0	0.0	2.741810800728942	0.0
23	0.0	0.0	0.0	2.8736268387500083	0.0
24	0.0	0.0	0.0	2.9641398980262945	0.0
25	0.0	0.0	0.0	3.0391178279098368	0.0
26	0.0	0.0	0.0	3.1151190297893083	0.0
27	0.0	0.0	0.0	3.1958644927408155	0.0
28	0.0	0.0	0.0	3.286842675651615	0.0
29	0.0	0.0	0.0	3.3782859821969278	0.0
30	0.0	0.0	0.0	3.4975436820861354	0.0
31	0.0	0.0	0.0	3.606661686742953	0.0
32	0.0	0.0	0.0	3.7086167874282663	0.0
33	0.0	0.0	0.0	3.81373472882827	0.0
34	0.0	0.0	0.0	3.9250853269307515	0.0
35	0.0	0.0	0.0	4.05243617806049	0.0
36	0.0	0.0	0.0	4.168903136142618	0.0
37	0.0	0.0	0.0	4.281463055694834	0.0
38	0.0	0.0	0.0	4.409372055185989	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACCGA	25	4.442647E-5	44.0	40
TCGATAT	25	4.442647E-5	44.0	16
TCATACG	25	4.442647E-5	44.0	34
CTCGATA	45	4.802132E-10	43.999996	24
ACGGTCT	230	0.0	43.04348	30
CGTTTTA	2130	0.0	42.760563	1
CGTTATT	1115	0.0	42.61883	1
CACGACG	235	0.0	42.12766	26
CGGTCTA	235	0.0	42.12766	31
TTACGGG	350	0.0	41.485718	3
AGGCGAT	985	0.0	41.319794	7
GTTTTAT	2480	0.0	41.072582	2
ACGGGTA	60	3.6379788E-12	40.333332	5
AGCGTAA	50	1.3478711E-9	39.600002	1
CGCATGG	50	1.3478711E-9	39.600002	2
CGTTCGA	50	1.3478711E-9	39.600002	14
TCGATAC	50	1.3478711E-9	39.600002	25
CCAGTCG	50	1.3478711E-9	39.600002	28
ATAGGCC	145	0.0	39.448273	5
AAGGCGA	605	0.0	39.272728	6
>>END_MODULE