FastQCFastQC Report
Thu 26 May 2016
SRR1545775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545775_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences411399
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8550.20782743759707728No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8500.20661207246493066No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC8120.1973752974606161No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGCGG253.886423E-545.0000042
TATCTAG253.886423E-545.0000042
AAGGCCG351.2093915E-745.0000046
ACGACTA253.886423E-545.00000439
TCGTTCA207.027999E-445.016
AACCCGA207.027999E-445.024
TACGGGA406.7975634E-945.04
GCCGACC207.027999E-445.014
CCTCATA207.027999E-445.015
ACCAGTC207.027999E-445.010
GCGATAC207.027999E-445.09
ACAGACG207.027999E-445.01
CGGTCTA850.045.031
GGCGATA1850.045.08
AACTAAT207.027999E-445.039
TTCGAAT207.027999E-445.016
ACGGTCT850.045.030
CGTAAAG302.1620908E-644.9999962
AGGCGAT3350.044.3283587
CGTTTTA6350.043.9370081