##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545775_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	411399
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95928769880335	31.0	31.0	33.0	30.0	33.0
2	31.117518516087788	31.0	31.0	33.0	30.0	34.0
3	31.07393552244901	31.0	31.0	34.0	28.0	34.0
4	35.06628358357701	35.0	35.0	37.0	32.0	37.0
5	32.75171548788403	35.0	35.0	37.0	28.0	37.0
6	33.940223481340496	35.0	35.0	37.0	28.0	37.0
7	15.328036772087438	0.0	0.0	35.0	0.0	37.0
8	24.90145819508555	17.0	17.0	35.0	17.0	37.0
9	32.619364655723516	32.0	32.0	37.0	27.0	39.0
10	35.40593681559751	37.0	34.0	37.0	32.0	39.0
11	36.391447232492055	37.0	35.0	39.0	32.0	39.0
12	36.27039686533025	37.0	35.0	39.0	32.0	39.0
13	35.98529165117076	37.0	35.0	39.0	31.0	39.0
14	37.035649090056125	39.0	35.0	40.0	31.0	41.0
15	37.32666097875785	39.0	36.0	40.0	32.0	41.0
16	37.35107523353241	39.0	35.0	41.0	32.0	41.0
17	37.266480958874475	39.0	35.0	41.0	32.0	41.0
18	37.23571277518905	39.0	36.0	40.0	32.0	41.0
19	37.18880697327898	39.0	35.0	40.0	32.0	41.0
20	37.30507852474119	39.0	35.0	40.0	32.0	41.0
21	37.153281850466335	39.0	35.0	40.0	32.0	41.0
22	37.213466731810236	39.0	35.0	40.0	32.0	41.0
23	37.215061290863616	39.0	35.0	40.0	32.0	41.0
24	37.03435108009499	39.0	35.0	40.0	32.0	41.0
25	36.80974917294403	38.0	35.0	40.0	31.0	41.0
26	36.66723059608798	38.0	35.0	40.0	31.0	41.0
27	36.582536661489215	38.0	35.0	40.0	31.0	41.0
28	36.46403126891412	38.0	35.0	40.0	31.0	41.0
29	36.46047511053746	38.0	35.0	40.0	31.0	41.0
30	36.17087061465876	38.0	35.0	40.0	30.0	41.0
31	36.05798020899419	38.0	35.0	40.0	30.0	41.0
32	35.97547879309381	38.0	35.0	40.0	30.0	41.0
33	35.788764678572385	38.0	34.0	40.0	29.0	41.0
34	35.62324896268586	38.0	34.0	40.0	28.0	41.0
35	35.47512755257062	38.0	34.0	40.0	27.0	41.0
36	35.28201332526331	38.0	34.0	40.0	26.0	41.0
37	35.16934168532252	38.0	34.0	40.0	26.0	41.0
38	35.05865109054713	38.0	34.0	40.0	26.0	41.0
39	34.96285601083133	38.0	34.0	40.0	25.0	41.0
40	34.79440640351581	38.0	34.0	40.0	24.0	41.0
41	34.66404633944176	37.0	33.0	40.0	24.0	41.0
42	34.60638212538193	37.0	33.0	40.0	24.0	41.0
43	34.51609264971476	37.0	33.0	40.0	23.0	41.0
44	34.29821657320509	37.0	33.0	40.0	23.0	41.0
45	34.20915947778191	37.0	33.0	40.0	23.0	41.0
46	34.18073937953179	37.0	33.0	40.0	23.0	41.0
47	34.104742597818664	37.0	33.0	40.0	23.0	41.0
48	34.074516466982175	36.0	33.0	40.0	23.0	41.0
49	33.94512140282305	36.0	33.0	40.0	23.0	41.0
50	33.815407912999305	36.0	33.0	40.0	23.0	41.0
51	33.62414833288365	36.0	32.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	10.0
15	20.0
16	52.0
17	105.0
18	235.0
19	525.0
20	870.0
21	1432.0
22	2102.0
23	3105.0
24	4348.0
25	5698.0
26	6941.0
27	7738.0
28	8713.0
29	10167.0
30	12580.0
31	16122.0
32	21055.0
33	30397.0
34	38347.0
35	41266.0
36	49139.0
37	73472.0
38	63670.0
39	13286.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3598307239444	23.475020600438988	27.94513355647437	14.220015119142243
2	33.08272504308469	23.993495365812752	30.18456534896779	12.739214242134764
3	31.743392667459087	23.462866949117522	31.684082849010327	13.109657534413063
4	28.647371529828707	26.956798631012713	29.09681355569654	15.299016283462041
5	21.641763835108982	37.1461768259038	27.38023184305261	13.831827495934604
6	20.075887398851236	42.32120155858424	27.683586980036413	9.919324062528105
7	38.39143994030126	56.75293328374643	3.9088573380100584	0.9467694379422411
8	87.22432480390083	3.6485261267042457	7.070508192776356	2.056640876618563
9	82.55221816290268	5.290727493260801	8.724620137627948	3.4324342062085713
10	47.705269093993905	25.047460008410326	14.444614595562946	12.802656302032819
11	43.29689668667157	21.934909904982757	20.284687128554033	14.483506279791639
12	39.44929375132171	20.696452835325317	24.698893288510668	15.155360124842307
13	24.21153187051986	33.73075773154529	25.065690485392526	16.992019912542325
14	18.119392609121558	37.30830653453217	27.817763290625404	16.75453756572087
15	17.12157783562916	23.521204475460564	42.4070063369138	16.95021135199648
16	19.545258982156007	18.574911460650124	41.9495429011738	19.93028665602007
17	18.13640772097161	18.904275411461864	29.950729097542773	33.00858777002375
18	22.588290200024794	23.536031930072753	32.65127042117263	21.22440744872982
19	29.62987270265606	25.38047005461851	24.452417239711327	20.537240003014105
20	31.722245314159732	22.93005087518443	24.17507091655546	21.172632894100374
21	24.776190510915193	27.407212948986263	27.148340175839024	20.668256364259516
22	23.32334303194709	23.451928662928204	25.889221898935098	27.335506406189612
23	22.30924236568392	29.26672160117064	24.581245943718873	23.842790089426565
24	21.928590006295593	24.21760869618059	35.2458319052793	18.60796939224451
25	21.694510681844147	22.823098743555526	32.239990860454206	23.24239971414612
26	18.93077037134266	31.177032515878743	26.855194105965257	23.037003006813336
27	20.308994431196965	29.998371410722925	27.787136089295306	21.905498068784805
28	18.842777935775246	27.824569335365425	34.51369594967416	18.81895677918517
29	20.22634960221099	25.194033043347215	33.607033561092756	20.972583793349038
30	21.763543421350075	27.09826713239459	31.063274339509817	20.074915106745518
31	29.881696358036848	24.3568895403246	24.898213170182718	20.86320093145584
32	30.37270387142409	25.24313379468594	25.740704279786776	18.643458054103192
33	28.74022542592471	25.09242851829975	24.9701627860058	21.197183269769738
34	21.372925067878143	26.76695859737141	27.95193960121439	23.908176733536056
35	22.08269830505179	24.212747235652007	30.23561068451795	23.468943774778257
36	28.926905510222433	26.100938504955046	24.862481435297607	20.109674549524915
37	22.257467811054475	31.315097994890607	26.285917078067765	20.141517115987156
38	22.301464028838183	31.870519860281625	22.829904788295547	22.998111322584645
39	22.697673061917992	29.04795587738424	25.22441717165088	23.029953889046887
40	24.331853018602377	24.895053220839138	26.633268432835276	24.13982532772321
41	19.818229990836148	24.22222708368275	27.42009581938702	28.539447106094084
42	22.62207735069847	26.026801231894098	23.632531921565196	27.718589495842238
43	22.46991363615371	25.680908315285162	24.710560793779273	27.138617254781856
44	21.728540905544254	27.42422806083632	28.00614488610813	22.841086147511298
45	20.62231556226437	32.13765711632745	24.208128848149848	23.031898473258323
46	22.57200430725403	30.103135885113964	26.19282010894533	21.13203969868668
47	22.86587959620709	27.1986562923099	25.703514106743086	24.231950004739925
48	22.37292749860841	25.134237078845594	29.51708681839285	22.975748604153146
49	22.14711265705556	24.558154006208085	30.315095564160337	22.979637772576016
50	20.473068724036764	29.454130904547654	27.170702894270526	22.90209747714506
51	19.87194912967703	30.54820259650607	25.69063123634233	23.88921703747457
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	440.0
2	647.0
3	2178.0
4	3709.0
5	2767.0
6	1825.0
7	1851.0
8	1877.0
9	1887.0
10	1897.0
11	1850.0
12	1803.0
13	1762.0
14	1721.0
15	1660.0
16	1599.0
17	1473.5
18	1348.0
19	1335.5
20	1323.0
21	1444.5
22	1566.0
23	1655.5
24	1745.0
25	2079.0
26	2809.5
27	3206.0
28	4088.0
29	4970.0
30	5723.0
31	6476.0
32	7318.5
33	8161.0
34	8657.5
35	9154.0
36	10225.5
37	11297.0
38	15030.5
39	18764.0
40	23667.5
41	28571.0
42	30419.5
43	32268.0
44	32734.5
45	33201.0
46	33184.0
47	33167.0
48	31652.5
49	30138.0
50	29107.5
51	28077.0
52	26412.5
53	24748.0
54	24152.0
55	23556.0
56	22595.5
57	21635.0
58	20896.0
59	20157.0
60	17797.5
61	15438.0
62	13229.0
63	11020.0
64	9421.5
65	7823.0
66	6597.5
67	5372.0
68	4626.5
69	3881.0
70	3368.0
71	2855.0
72	2302.5
73	1750.0
74	1377.0
75	763.5
76	523.0
77	381.5
78	240.0
79	178.0
80	116.0
81	92.5
82	69.0
83	49.0
84	29.0
85	19.5
86	10.0
87	9.5
88	9.0
89	7.5
90	6.0
91	3.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411399.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.70415154565209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.01652759767508	24.96488936554094
2	8.9831582588317	6.594384041814699
3	4.942680977589664	5.442507349297886
4	3.3284784473713738	4.886759093950228
5	2.476779662479645	4.545404803842096
6	1.9696172248959285	4.337587746570413
7	1.6536908941017125	4.248812483074191
8	1.3826375445712868	4.0598830374922255
9	1.0652721276217672	3.518991864862972
>10	6.122146685552462	34.75112187623038
>50	0.03540634758563918	0.8266645221351262
>100	0.021637212413446166	1.217254484122116
>500	0.001967019310313288	0.6057393310666994
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	855	0.20782743759707728	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	850	0.20661207246493066	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	812	0.1973752974606161	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009722921057173206	0.0
2	0.0	0.0	0.0	0.038162465149404835	0.0
3	0.0	0.0	0.0	0.05031611647087134	0.0
4	0.0	0.0	0.0	0.07802644148381498	0.0
5	0.0	0.0	0.0	0.1378224059854302	0.0
6	0.0	0.0	0.0	0.20661207246493063	0.0
7	0.0	0.0	0.0	0.2622757955172472	0.0
8	0.0	0.0	0.0	0.6485188345134528	0.0
9	0.0	0.0	0.0	0.8356850648640372	0.0
10	0.0	0.0	0.0	1.0952870570905617	0.0
11	0.0	0.0	0.0	1.2878008940225911	0.0
12	0.0	0.0	0.0	1.4586812316024103	0.0
13	0.0	0.0	0.0	1.5354923079540785	0.0
14	0.0	0.0	0.0	1.5595565375705824	0.0
15	0.0	0.0	0.0	1.5935867612706887	0.0
16	0.0	0.0	0.0	1.6742870060452262	0.0
17	0.0	0.0	0.0	1.7836698679384246	0.0
18	0.0	0.0	0.0	1.9584393739411132	0.0
19	0.0	0.0	0.0	2.0260136752884668	0.0
20	0.0	0.0	0.0	2.0943171957151088	0.0
21	0.0	0.0	0.0	2.1837680694411024	0.0
22	0.0	0.0	0.0	2.2744343082992424	0.0
23	0.0	0.0	0.0	2.389650922826745	0.0
24	0.0	0.0	0.0	2.4732680439184342	0.0
25	0.0	0.0	0.0	2.5393839071072124	0.0
26	0.0	0.0	0.0	2.6064720624017075	0.0
27	0.0	0.0	0.0	2.6638372966390294	0.0
28	0.0	0.0	0.0	2.738703788779263	0.0
29	0.0	0.0	0.0	2.8164871572366486	0.0
30	0.0	0.0	0.0	2.92125163162769	0.0
31	0.0	0.0	0.0	3.016293184961558	0.0
32	0.0	0.0	0.0	3.10987630013685	0.0
33	0.0	0.0	0.0	3.2022440501799956	0.0
34	0.0	0.0	0.0	3.2897503396945544	0.0
35	0.0	0.0	0.0	3.4015639318520465	0.0
36	0.0	0.0	0.0	3.4958762661066265	0.0
37	0.0	0.0	0.0	3.6001545944448092	0.0
38	0.0	0.0	0.0	3.7088082372587197	0.0
39	0.0	0.0	0.0	3.8303447504733845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGCGG	25	3.886423E-5	45.000004	2
TATCTAG	25	3.886423E-5	45.000004	2
AAGGCCG	35	1.2093915E-7	45.000004	6
ACGACTA	25	3.886423E-5	45.000004	39
TCGTTCA	20	7.027999E-4	45.0	16
AACCCGA	20	7.027999E-4	45.0	24
TACGGGA	40	6.7975634E-9	45.0	4
GCCGACC	20	7.027999E-4	45.0	14
CCTCATA	20	7.027999E-4	45.0	15
ACCAGTC	20	7.027999E-4	45.0	10
GCGATAC	20	7.027999E-4	45.0	9
ACAGACG	20	7.027999E-4	45.0	1
CGGTCTA	85	0.0	45.0	31
GGCGATA	185	0.0	45.0	8
AACTAAT	20	7.027999E-4	45.0	39
TTCGAAT	20	7.027999E-4	45.0	16
ACGGTCT	85	0.0	45.0	30
CGTAAAG	30	2.1620908E-6	44.999996	2
AGGCGAT	335	0.0	44.328358	7
CGTTTTA	635	0.0	43.937008	1
>>END_MODULE