FastQCFastQC Report
Thu 26 May 2016
SRR1545774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545774_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences761799
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24260.3184567057714699No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10610.13927558319189182No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.12549241991653964No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA8550.11223432952786759No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTAA207.854807E-444.0000041
CGTATGG408.305506E-944.0000042
CGTATCG207.854807E-444.00000433
CGCGAAT207.854807E-444.00000412
CGTTCGC207.854807E-444.00000442
GTCGTAT207.854807E-444.00000431
GTGCGTA207.854807E-444.00000416
ATTTACG302.526589E-644.01
GCGACTA302.526589E-644.01
CCGGATA254.441422E-544.020
TTCACGG254.441422E-544.02
AATCTCG254.441422E-544.018
AGGCGAT4550.043.0329677
CGTTTTA15850.043.0283931
ACGGGAT1550.042.5806475
CGTTATT9650.042.404151
TCACGAC1250.042.2425
TAATGGG4000.040.6999973
CACGACC650.040.61538327
ACGGGTG650.040.6153835
Produced by FastQC (version 0.11.5)