##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545774_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	761799
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.49276121391601	31.0	31.0	33.0	30.0	34.0
2	31.68925661493386	31.0	31.0	34.0	30.0	34.0
3	31.747744483781155	31.0	31.0	34.0	30.0	34.0
4	35.64860941009374	37.0	35.0	37.0	33.0	37.0
5	27.23253902932401	35.0	26.0	37.0	0.0	37.0
6	31.403073514142182	35.0	28.0	37.0	17.0	37.0
7	14.84641880601051	0.0	0.0	32.0	0.0	37.0
8	25.01284984621928	17.0	17.0	35.0	17.0	37.0
9	33.159745549679116	32.0	32.0	37.0	28.0	39.0
10	35.98694274999048	37.0	35.0	39.0	32.0	39.0
11	36.95605796279596	38.0	37.0	39.0	34.0	39.0
12	37.012687073624406	39.0	35.0	39.0	33.0	39.0
13	36.599164609037295	39.0	35.0	39.0	33.0	39.0
14	37.91783265664565	40.0	36.0	41.0	33.0	41.0
15	38.1929301561173	40.0	37.0	41.0	33.0	41.0
16	38.24579711971268	40.0	37.0	41.0	34.0	41.0
17	38.24221612262552	40.0	36.0	41.0	34.0	41.0
18	38.221377292435406	40.0	36.0	41.0	34.0	41.0
19	38.17403672097233	40.0	36.0	41.0	34.0	41.0
20	38.2191522960781	40.0	36.0	41.0	34.0	41.0
21	38.09490036085634	40.0	36.0	41.0	34.0	41.0
22	38.004684962831405	40.0	36.0	41.0	34.0	41.0
23	37.89531621858259	40.0	36.0	41.0	34.0	41.0
24	37.75543942693545	40.0	35.0	41.0	33.0	41.0
25	37.57622679998267	39.0	35.0	41.0	33.0	41.0
26	37.333324144557814	39.0	35.0	40.0	33.0	41.0
27	37.12296550664939	39.0	35.0	40.0	33.0	41.0
28	37.15473110361132	39.0	35.0	40.0	32.0	41.0
29	37.078931581690185	39.0	35.0	40.0	32.0	41.0
30	36.88900746784913	39.0	35.0	40.0	32.0	41.0
31	36.718872038424834	38.0	35.0	40.0	31.0	41.0
32	36.50480769861866	38.0	35.0	40.0	31.0	41.0
33	36.29353805925185	38.0	35.0	40.0	31.0	41.0
34	36.05064196723808	38.0	35.0	40.0	30.0	41.0
35	35.866052593925694	38.0	35.0	40.0	30.0	41.0
36	35.69802270677698	38.0	35.0	40.0	29.0	41.0
37	35.57777576499838	38.0	35.0	40.0	29.0	41.0
38	35.41188686254511	38.0	34.0	40.0	28.0	41.0
39	35.340728984942224	38.0	34.0	40.0	28.0	41.0
40	35.12997522968657	38.0	34.0	40.0	27.0	41.0
41	34.957389022563696	38.0	34.0	40.0	27.0	41.0
42	34.98191123905387	38.0	34.0	40.0	27.0	41.0
43	34.85398904435422	37.0	34.0	40.0	26.0	41.0
44	34.62195671036586	37.0	34.0	40.0	26.0	40.0
45	34.407656087760685	37.0	33.0	40.0	25.0	40.0
46	34.25874147905156	36.0	33.0	39.0	24.0	40.0
47	34.07877799787083	36.0	33.0	39.0	24.0	40.0
48	34.08188774204219	36.0	33.0	39.0	24.0	40.0
49	33.94073502328042	36.0	33.0	39.0	24.0	40.0
50	34.107540177920946	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	16.0
16	38.0
17	136.0
18	257.0
19	526.0
20	1038.0
21	1655.0
22	2672.0
23	4083.0
24	6256.0
25	8925.0
26	11219.0
27	12511.0
28	13050.0
29	14855.0
30	18275.0
31	24212.0
32	33510.0
33	59142.0
34	62988.0
35	79417.0
36	107798.0
37	194708.0
38	83017.0
39	21491.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.44924186038575	23.762436023150464	28.571972396918348	15.216349719545445
2	34.44412502510505	23.67291109597151	29.121067368164045	12.7618965107594
3	30.80563245685542	23.652433253390985	32.35394113145331	13.187993158300287
4	27.45172939318639	26.29473128738683	31.28764936682773	14.965889952599046
5	17.7456258146834	47.74395870826819	22.760203150699855	11.750212326348551
6	20.682621006328443	40.5205310062103	28.38570279036859	10.41114519709267
7	39.13066307516812	56.147225186696225	3.740750512930576	0.9813612252050737
8	88.21959598266734	2.7524320719769912	7.336449640915779	1.6915223044398848
9	83.95088468217995	4.1982202654505985	9.034272819995826	2.816622232373631
10	48.33072765913318	25.18617115538351	14.508551468300693	11.974549717182617
11	39.854476049456615	22.296826328204684	21.276609709385284	16.572087912953418
12	35.55898603174853	20.830954096815564	27.393577570986572	16.216482300449332
13	21.69758689628104	33.04861256053106	29.774914380302413	15.478886162885486
14	16.35575788364122	34.39844368396388	32.032859061248445	17.212939371146458
15	15.388573626376512	21.998716196792067	45.222689974652106	17.390020202179315
16	19.72121255081721	16.869016630371004	43.35789361760779	20.05187720120399
17	19.894486603421637	18.81257392041733	30.359976844285697	30.932962631875338
18	22.037965395071403	22.642061751196838	34.380459937595084	20.93951291613667
19	26.953697760170332	23.4128687488432	29.448712849452413	20.184720641534053
20	28.760211026793158	21.980469913979935	26.73894294951818	22.520376109708728
21	23.204808617496216	24.795123123028514	30.101772252260766	21.8982960072145
22	25.05359025149679	22.21360227566589	28.242751696969936	24.490055775867386
23	22.06986357293722	28.699433840159937	25.523924289740467	23.706778297162376
24	20.468128732119627	24.807462335865495	34.49098778024124	20.23342115177363
25	20.17238142869707	25.37191568904659	31.47890716580095	22.97679571645539
26	20.15190358611655	30.31232647981948	27.613845646948864	21.921924287115104
27	19.977841924182098	29.84711190222093	29.165961099975192	21.009085073621783
28	18.430058322470888	26.653224800767656	34.35302487926605	20.563691997495404
29	18.411286966772074	23.852879827881107	35.86077167336791	21.875061531978908
30	20.637070933408943	25.09441466843616	33.252209572341265	21.016304825813634
31	27.550180559438907	25.59375898366892	25.340673852289118	21.51538660460305
32	29.536006216862976	25.83279841533003	25.84264353195528	18.788551835851713
33	29.210986100008007	24.542169259870388	25.626444770864754	20.62039986925685
34	22.432557669411484	26.935188940914863	28.230937557019637	22.40131583265402
35	21.17343288715265	26.948447031303534	29.804187193734833	22.07393288780899
36	29.7495796135201	25.92271714717399	24.531667802136784	19.79603543716912
37	22.33515664893233	30.953703010899204	26.09585993155675	20.61528040861172
38	22.158075817899473	32.55530658349512	23.705596883167345	21.58102071543806
39	20.764794913093873	31.0228813637193	25.00121423105045	23.211109492136377
40	22.80010869008754	25.95185869238474	25.817046228729623	25.430986388798093
41	19.108583760283224	24.058314594794687	26.687354538401863	30.145747106520226
42	21.448308543329674	27.61975271692402	23.29000169336006	27.64193704638625
43	21.82701736284768	27.18013544255112	26.535871010594658	24.456976184006542
44	23.14783820929143	27.6255285186775	27.433614378595927	21.793018893435146
45	22.48375227586279	30.837793171164574	24.073279172065075	22.605175380907564
46	23.85708041097455	27.167796229714135	27.668584495385268	21.30653886392605
47	23.86285621272803	25.880186243352902	27.626709932672526	22.63024761124654
48	23.481390760554948	23.791446300139537	28.48271000618273	24.24445293312278
49	20.926386093969672	23.870863574249903	30.88688748606916	24.31586284571127
50	20.644028149157457	27.819674218527457	27.838970647112955	23.69732698520213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	346.0
1	700.5
2	1055.0
3	4417.5
4	7780.0
5	5803.0
6	3826.0
7	3927.5
8	4029.0
9	4148.5
10	4268.0
11	4326.5
12	4385.0
13	4166.0
14	3947.0
15	3812.5
16	3678.0
17	3602.5
18	3527.0
19	3520.5
20	3514.0
21	3415.5
22	3317.0
23	3526.5
24	3736.0
25	4326.5
26	4917.0
27	5686.0
28	6455.0
29	7621.5
30	8788.0
31	10624.5
32	12461.0
33	14648.0
34	16835.0
35	17761.5
36	18688.0
37	21211.5
38	23735.0
39	31385.0
40	39035.0
41	48468.5
42	57902.0
43	61379.0
44	64856.0
45	67535.0
46	70214.0
47	69589.5
48	68965.0
49	65576.5
50	62188.0
51	55924.0
52	49660.0
53	45328.5
54	40997.0
55	39139.0
56	37281.0
57	34965.5
58	32650.0
59	29993.5
60	27337.0
61	24914.0
62	22491.0
63	19300.5
64	16110.0
65	13442.0
66	10774.0
67	9485.0
68	8196.0
69	7128.5
70	6061.0
71	4903.5
72	3746.0
73	2871.0
74	1996.0
75	1504.0
76	1012.0
77	792.0
78	572.0
79	406.5
80	241.0
81	185.0
82	129.0
83	95.0
84	61.0
85	43.0
86	25.0
87	16.5
88	8.0
89	6.0
90	4.0
91	2.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	761799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.500636787341953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.86647002828357	18.388705054995135
2	7.820875877306188	4.301581337213636
3	4.152738365768604	3.426088484095872
4	3.027456711974071	3.330279497015978
5	2.2573792981437033	3.1039684084757444
6	1.8367363438771118	3.0306851440244915
7	1.5609422805220188	3.0048834691848896
8	1.391019390182548	3.060313521084812
9	1.1554726303114489	2.8598609811518827
>10	9.7330900493898	50.81789566660334
>50	0.17550098048180635	3.0088209448085035
>100	0.020289130690108917	0.9278941550152513
>500	0.001014456534505446	0.25261820726587797
>1k	0.001014456534505446	0.48640512906466943
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2426	0.3184567057714699	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1061	0.13927558319189182	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	956	0.12549241991653964	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	855	0.11223432952786759	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.002756632655070432	0.0
2	0.0	0.0	0.0	0.014308236162032242	0.0
3	0.0	0.0	0.0	0.021134183688873313	0.0
4	0.0	0.0	0.0	0.03426100585587537	0.0
5	0.0	0.0	0.0	0.06576537905668031	0.0
6	0.0	0.0	0.0	0.13796290097519162	0.0
7	0.0	0.0	0.0	0.18587580188474911	0.0
8	0.0	0.0	0.0	0.4671836009236032	0.0
9	0.0	0.0	0.0	0.605671574785475	0.0
10	0.0	0.0	0.0	0.8090060501523367	0.0
11	0.0	0.0	0.0	0.9782107878849933	0.0
12	0.0	0.0	0.0	1.1223432952786758	0.0
13	0.0	0.0	0.0	1.1769508754934044	0.0
14	0.0	0.0	0.0	1.2005791553940082	0.0
15	0.0	0.0	0.0	1.2285392866097224	0.0
16	0.0	0.0	0.0	1.3069064149467249	0.0
17	0.0	0.0	0.0	1.3966938785690188	0.0
18	1.3126822167002056E-4	0.0	0.0	1.5278308320173695	0.0
19	1.3126822167002056E-4	0.0	0.0	1.582307144010428	0.0
20	1.3126822167002056E-4	0.0	0.0	1.6430843306436476	0.0
21	1.3126822167002056E-4	0.0	0.0	1.72197653186733	0.0
22	1.3126822167002056E-4	0.0	0.0	1.8091386310562234	0.0
23	1.3126822167002056E-4	0.0	0.0	1.9027328731069482	0.0
24	1.3126822167002056E-4	0.0	0.0	1.9733551763654191	0.0
25	1.3126822167002056E-4	0.0	0.0	2.0322946078952584	0.0
26	1.3126822167002056E-4	0.0	0.0	2.091627844090108	0.0
27	1.3126822167002056E-4	0.0	0.0	2.151092348506627	0.0
28	1.3126822167002056E-4	0.0	0.0	2.213444753799887	0.0
29	1.3126822167002056E-4	0.0	0.0	2.2830169112849976	0.0
30	1.3126822167002056E-4	0.0	0.0	2.387112611069324	0.0
31	1.3126822167002056E-4	0.0	0.0	2.4720431504898275	0.0
32	1.3126822167002056E-4	0.0	0.0	2.5502790106051596	0.0
33	1.3126822167002056E-4	0.0	0.0	2.635472086469003	0.0
34	1.3126822167002056E-4	0.0	0.0	2.7176459932344357	0.0
35	1.3126822167002056E-4	0.0	0.0	2.8242357892304923	0.0
36	1.3126822167002056E-4	0.0	0.0	2.915861007956167	0.0
37	1.3126822167002056E-4	0.0	0.0	3.0164124657554026	0.0
38	1.3126822167002056E-4	0.0	0.0	3.1220833841997693	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTAA	20	7.854807E-4	44.000004	1
CGTATGG	40	8.305506E-9	44.000004	2
CGTATCG	20	7.854807E-4	44.000004	33
CGCGAAT	20	7.854807E-4	44.000004	12
CGTTCGC	20	7.854807E-4	44.000004	42
GTCGTAT	20	7.854807E-4	44.000004	31
GTGCGTA	20	7.854807E-4	44.000004	16
ATTTACG	30	2.526589E-6	44.0	1
GCGACTA	30	2.526589E-6	44.0	1
CCGGATA	25	4.441422E-5	44.0	20
TTCACGG	25	4.441422E-5	44.0	2
AATCTCG	25	4.441422E-5	44.0	18
AGGCGAT	455	0.0	43.032967	7
CGTTTTA	1585	0.0	43.028393	1
ACGGGAT	155	0.0	42.580647	5
CGTTATT	965	0.0	42.40415	1
TCACGAC	125	0.0	42.24	25
TAATGGG	400	0.0	40.699997	3
CACGACC	65	0.0	40.615383	27
ACGGGTG	65	0.0	40.615383	5
>>END_MODULE