FastQCFastQC Report
Thu 26 May 2016
SRR1545773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545773_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences288784
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.21053797994348716No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.19841819491384563No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3280.11357969970635493No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.883527E-545.0000042
CACGACG253.883527E-545.00000426
TAGCCGT253.883527E-545.00000444
GTAGCCG253.883527E-545.00000443
GCTAGCG253.883527E-545.0000041
GATTGGA253.883527E-545.0000041
TAGGATG1150.045.0000046
AAGGCGC253.883527E-545.0000046
ACGGTCT253.883527E-545.00000430
CGTTGAT406.7848305E-945.025
ATTAATC406.7848305E-945.019
GCGTTAG207.0245046E-445.02
CCCCGAT207.0245046E-445.040
TATTACG207.0245046E-445.039
GGTTAAC207.0245046E-445.08
AATCGTT406.7848305E-945.022
TTATGCA302.159837E-644.9999961
TTACGGG302.159837E-644.9999963
CACAACG302.159837E-644.99999612
CGAAGGT302.159837E-644.9999964
Produced by FastQC (version 0.11.5)