##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545773_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	288784
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.977734223502686	31.0	31.0	33.0	30.0	33.0
2	31.140031996232477	31.0	31.0	33.0	30.0	34.0
3	31.099659260900882	31.0	31.0	34.0	28.0	34.0
4	35.07458861986814	35.0	35.0	37.0	32.0	37.0
5	32.83497008144496	35.0	35.0	37.0	28.0	37.0
6	34.00690135187545	35.0	35.0	37.0	28.0	37.0
7	14.874456341071527	0.0	0.0	35.0	0.0	37.0
8	24.673652279904704	17.0	17.0	35.0	17.0	37.0
9	32.58164579755111	32.0	32.0	37.0	27.0	39.0
10	35.4051159344008	37.0	34.0	37.0	32.0	39.0
11	36.37452559698598	37.0	35.0	39.0	32.0	39.0
12	36.32108080780099	37.0	35.0	39.0	32.0	39.0
13	36.08686076790958	37.0	35.0	39.0	32.0	39.0
14	37.14588412100393	39.0	36.0	40.0	32.0	41.0
15	37.4565523020666	39.0	36.0	41.0	32.0	41.0
16	37.489798603800764	39.0	36.0	41.0	32.0	41.0
17	37.40352651116405	39.0	35.0	41.0	32.0	41.0
18	37.34166712837276	39.0	36.0	40.0	32.0	41.0
19	37.309902903207934	39.0	36.0	40.0	32.0	41.0
20	37.436270707518425	39.0	35.0	41.0	32.0	41.0
21	37.31691160175079	39.0	35.0	41.0	32.0	41.0
22	37.344697074630176	39.0	35.0	41.0	32.0	41.0
23	37.32100116349936	39.0	35.0	41.0	32.0	41.0
24	37.15075627458585	39.0	35.0	41.0	32.0	41.0
25	36.885450717491274	39.0	35.0	40.0	31.0	41.0
26	36.761555349326834	38.0	35.0	40.0	31.0	41.0
27	36.66236702864425	38.0	35.0	40.0	31.0	41.0
28	36.56975455703917	38.0	35.0	40.0	31.0	41.0
29	36.563968225386446	38.0	35.0	40.0	31.0	41.0
30	36.3106439414926	38.0	35.0	40.0	30.0	41.0
31	36.17294240678154	38.0	35.0	40.0	30.0	41.0
32	36.0837130865976	38.0	35.0	40.0	30.0	41.0
33	35.90207906255194	38.0	35.0	40.0	29.0	41.0
34	35.7425134356474	38.0	35.0	40.0	29.0	41.0
35	35.56793658928473	38.0	34.0	40.0	27.0	41.0
36	35.38104950412765	38.0	34.0	40.0	26.0	41.0
37	35.22650493102111	38.0	34.0	40.0	26.0	41.0
38	35.12358025375367	38.0	34.0	40.0	26.0	41.0
39	35.024416172641146	38.0	34.0	40.0	25.0	41.0
40	34.88880962934235	38.0	34.0	40.0	24.0	41.0
41	34.76165230760707	38.0	34.0	40.0	24.0	41.0
42	34.732737963322066	38.0	33.0	40.0	24.0	41.0
43	34.63125034627957	38.0	33.0	40.0	24.0	41.0
44	34.30941811180675	37.0	33.0	40.0	23.0	41.0
45	34.252707906255196	37.0	33.0	40.0	23.0	41.0
46	34.17299434871738	37.0	33.0	40.0	23.0	41.0
47	34.101910078120675	37.0	33.0	40.0	23.0	41.0
48	34.12504847914012	37.0	33.0	40.0	23.0	41.0
49	34.04408138955067	36.0	33.0	40.0	23.0	41.0
50	33.97634564241786	36.0	33.0	40.0	23.0	41.0
51	33.813497285168154	36.0	33.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	10.0
16	31.0
17	86.0
18	181.0
19	381.0
20	586.0
21	977.0
22	1434.0
23	2227.0
24	2980.0
25	4005.0
26	4809.0
27	5090.0
28	5847.0
29	6807.0
30	8389.0
31	10720.0
32	14308.0
33	21020.0
34	26592.0
35	29332.0
36	35954.0
37	52820.0
38	45104.0
39	9084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.016662973017894	24.41651892071583	28.81877112305391	14.748046983212365
2	34.15701700925259	23.513421796221397	29.55911684857887	12.770444345947144
3	31.362887140561803	23.240553493268326	32.136129425452935	13.260429940716936
4	27.995664579755108	26.69088315142113	29.989196077345003	15.324256191478753
5	21.660479804975346	36.434151476536094	27.421879328494654	14.483489389993906
6	20.744224056734446	41.10061499252036	27.970039891406728	10.185121059338467
7	37.52216189262563	57.90452379633221	3.824311596210316	0.7490027148318467
8	88.75076181505901	3.020596708958945	6.611169593883318	1.6174718820987315
9	84.43854230151256	4.310834395257355	8.323868358357805	2.926754944872292
10	48.52727297911242	25.706410327441965	13.837331708127873	11.928984985317745
11	40.25915563189096	22.6733475538811	20.563466120006648	16.504030694221285
12	35.7789905257909	21.231439414926033	26.788533990802815	16.20103606848025
13	21.898027591556318	33.89696105047371	28.836777660812235	15.368233697157738
14	16.35616931685966	35.17092359687518	31.35838550612222	17.114521580142945
15	15.41775167599313	22.442725358745637	44.87817884647349	17.261344118787743
16	19.883719319629897	17.176505623580255	42.98576098398803	19.95401407280182
17	19.919732395146543	19.1156712283229	29.869037065765415	31.095559310765143
18	22.095060668181063	22.86622527563854	34.20757382680481	20.83114022937559
19	27.643844534323232	23.53766136628068	28.750554047315642	20.067940052080445
20	29.350656546069033	22.205523851736938	26.075890631059895	22.367928971134134
21	23.241938611557426	25.349049808853675	29.526912848357252	21.882098731231647
22	24.853523740927475	22.726328328439248	27.637611502022274	24.782536428611003
23	22.058008753947586	29.30979555654053	24.907889633774722	23.72430605573716
24	20.48209042052191	25.029433763643418	34.447199290819434	20.041276525015235
25	20.260471494265612	25.758698542855562	31.178666408111255	22.802163554767578
26	19.93843149204942	31.042924815779266	27.129965649066428	21.888678043104882
27	19.830392265499476	30.81507285722201	28.502964153138677	20.851570724139844
28	18.3109175023547	27.219998337858055	34.066291761316414	20.40279239847083
29	18.266939996675717	24.35349603856169	35.56741370713059	21.812150257632002
30	20.584935453487727	25.763546456867413	32.79509945149316	20.8564186381517
31	28.039988365006373	25.876779877001493	24.84313535375921	21.240096404232922
32	29.999238184940996	25.95746301734168	25.393373594104933	18.649925203612387
33	29.77554158125104	24.52248046983212	24.994113247271315	20.70786470164552
34	22.325336583744253	27.215496703418474	27.909787245830792	22.549379467006485
35	21.421200620532993	26.91111695938833	29.687240290320794	21.980442129757883
36	30.185190315252925	26.19916615878996	24.076472380741315	19.5391711452158
37	22.156698432046095	31.76872679926866	25.789863704360354	20.284711064324892
38	21.997063549227104	33.281622250540195	23.32643082719264	21.39488337304006
39	20.7476868524572	31.598357249709125	24.618053631780153	23.03590226605352
40	22.931672114798605	26.116059061443845	25.524267272425067	25.428001551332486
41	19.00901712006205	24.175162058839824	26.40935785916117	30.40646296193695
42	21.316624189705802	27.625145437420358	23.024821319740706	28.033409053133134
43	21.51781262119785	27.05620810017175	26.56172087096238	24.864258407668018
44	22.70555155410272	27.97800432156906	27.588786082331428	21.727658041996786
45	22.484625186990968	31.391628345060667	23.88497977727298	22.238766690675384
46	23.73192420632722	27.754653997451385	27.29063937060225	21.222782425619148
47	23.59341237741703	26.21093966424733	27.24216023048368	22.95348772785196
48	23.34270596708959	24.03699650950191	28.62069920771234	23.99959831569616
49	20.72621751897612	24.480234362014517	30.791525846307277	24.00202227270209
50	20.651421131364618	28.62104548728461	27.488018726799268	23.2395146545515
51	19.178001551332482	30.201811734722146	26.526054075018006	24.094132638927366
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	139.0
1	250.0
2	361.0
3	1395.5
4	2430.0
5	1824.5
6	1219.0
7	1249.5
8	1280.0
9	1324.5
10	1369.0
11	1388.0
12	1407.0
13	1334.0
14	1261.0
15	1270.0
16	1279.0
17	1255.5
18	1232.0
19	1289.0
20	1346.0
21	1294.0
22	1242.0
23	1312.0
24	1382.0
25	1577.0
26	2063.5
27	2355.0
28	2674.0
29	2993.0
30	3604.0
31	4215.0
32	5084.0
33	5953.0
34	6447.5
35	6942.0
36	7572.5
37	8203.0
38	10681.0
39	13159.0
40	16540.5
41	19922.0
42	21889.0
43	23856.0
44	24453.0
45	25050.0
46	25457.5
47	25865.0
48	24974.5
49	24084.0
50	22419.5
51	20755.0
52	18761.5
53	16768.0
54	15734.0
55	14700.0
56	13916.5
57	13133.0
58	12140.5
59	11148.0
60	10205.5
61	9263.0
62	8156.0
63	7049.0
64	6021.5
65	4994.0
66	4261.5
67	3529.0
68	3017.5
69	2506.0
70	2271.5
71	2037.0
72	1628.0
73	1219.0
74	961.0
75	514.0
76	325.0
77	252.5
78	180.0
79	129.0
80	78.0
81	57.0
82	36.0
83	30.5
84	25.0
85	20.0
86	15.0
87	9.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	288784.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.553043536369714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.04179448424711	22.31263371320817
2	11.632462298579881	8.504038016703392
3	6.5786955157896605	7.21414030803539
4	4.492414828892047	6.5684573929569545
5	3.1914433071795005	5.832848307559401
6	2.5836752704165655	5.66647167860271
7	2.0302340456145793	5.194786342085881
8	1.6799063385299167	4.912455162344599
9	1.2649039326931535	4.161247966895229
>10	5.471628266534493	28.254933363701163
>50	0.02587528786667577	0.6631656981084273
>100	0.004976016897437648	0.2852012656703275
>500	0.001990406758975059	0.4296207841283888
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	608	0.21053797994348716	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	0.19841819491384563	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	328	0.11357969970635493	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0038090752950301955	0.0
2	0.0	0.0	0.0	0.016275139896947198	0.0
3	0.0	0.0	0.0	0.022508172197905702	0.0
4	0.0	0.0	0.0	0.037051914233475536	0.0
5	0.0	0.0	0.0	0.06717823702144163	0.0
6	0.0	0.0	0.0	0.11461853842318134	0.0
7	0.0	0.0	0.0	0.1440523020665965	0.0
8	0.0	0.0	0.0	0.37225054019613274	0.0
9	0.0	0.0	0.0	0.4813286054629065	0.0
10	0.0	0.0	0.0	0.6489279184442351	0.0
11	0.0	0.0	0.0	0.7877860269266995	0.0
12	0.0	0.0	0.0	0.9062136406449111	0.0
13	0.0	0.0	0.0	0.9619646517812621	0.0
14	0.0	0.0	0.0	0.9858579422682697	0.0
15	0.0	0.0	0.0	1.0163305446285114	0.0
16	0.0	0.0	0.0	1.0769294697767189	0.0
17	0.0	0.0	0.0	1.15103329824367	0.0
18	0.0	0.0	0.0	1.270499750678708	0.0
19	0.0	0.0	0.0	1.3186326112249986	0.0
20	0.0	0.0	0.0	1.376115020222727	0.0
21	0.0	0.0	0.0	1.4422544185273423	0.0
22	0.0	0.0	0.0	1.5250152363011802	0.0
23	0.0	0.0	0.0	1.6050058174968143	0.0
24	0.0	0.0	0.0	1.6624882264945426	0.0
25	0.0	0.0	0.0	1.7165078397695164	0.0
26	0.0	0.0	0.0	1.7715662917613164	0.0
27	0.0	0.0	0.0	1.8252396254640146	0.0
28	0.0	0.0	0.0	1.8872236689013242	0.0
29	0.0	0.0	0.0	1.945052357471328	0.0
30	0.0	0.0	0.0	2.0298908526788186	0.0
31	0.0	0.0	0.0	2.0984542079893624	0.0
32	0.0	0.0	0.0	2.1711729181672115	0.0
33	0.0	0.0	0.0	2.2421602304836834	0.0
34	0.0	0.0	0.0	2.314532661089257	0.0
35	0.0	0.0	0.0	2.414953737049144	0.0
36	0.0	0.0	0.0	2.500831070973461	0.0
37	0.0	0.0	0.0	2.5887860823314313	0.0
38	0.0	0.0	0.0	2.681935287273533	0.0
39	0.0	0.0	0.0	2.8097124494431824	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	25	3.883527E-5	45.000004	2
CACGACG	25	3.883527E-5	45.000004	26
TAGCCGT	25	3.883527E-5	45.000004	44
GTAGCCG	25	3.883527E-5	45.000004	43
GCTAGCG	25	3.883527E-5	45.000004	1
GATTGGA	25	3.883527E-5	45.000004	1
TAGGATG	115	0.0	45.000004	6
AAGGCGC	25	3.883527E-5	45.000004	6
ACGGTCT	25	3.883527E-5	45.000004	30
CGTTGAT	40	6.7848305E-9	45.0	25
ATTAATC	40	6.7848305E-9	45.0	19
GCGTTAG	20	7.0245046E-4	45.0	2
CCCCGAT	20	7.0245046E-4	45.0	40
TATTACG	20	7.0245046E-4	45.0	39
GGTTAAC	20	7.0245046E-4	45.0	8
AATCGTT	40	6.7848305E-9	45.0	22
TTATGCA	30	2.159837E-6	44.999996	1
TTACGGG	30	2.159837E-6	44.999996	3
CACAACG	30	2.159837E-6	44.999996	12
CGAAGGT	30	2.159837E-6	44.999996	4
>>END_MODULE