FastQCFastQC Report
Thu 26 May 2016
SRR1545772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545772_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences719339
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.5029617468259054No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15400.21408543120837323No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14570.20254706056532457No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA9110.12664404404599222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT408.305506E-944.06
ACACGAC408.305506E-944.026
ACGCCCG207.8545156E-444.027
CCGGATA207.8545156E-444.020
CGATTAC207.8545156E-444.010
CGTTCGA408.305506E-944.014
TTATACG207.8545156E-444.01
TCTACGG302.5263907E-644.02
TTGCACG254.441172E-544.01
CTAGGCG408.305506E-944.05
CGTTTTA18700.043.2941171
CGGTCTA1400.042.42857431
CGTTATT11050.041.8099561
CGAATAT800.041.2514
GTTATTT11850.041.215192
GACGGTC1550.041.1612929
TAAGGAC2850.040.912285
GTTTTAT21500.040.8279082
TTACTGG9850.040.6497540
ACGGGTA650.040.6153875
Produced by FastQC (version 0.11.5)