##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545772_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	719339
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53995821163596	31.0	31.0	33.0	30.0	34.0
2	31.73054568152151	31.0	31.0	34.0	30.0	34.0
3	31.778004529158018	31.0	31.0	34.0	30.0	34.0
4	35.68618968247238	37.0	35.0	37.0	35.0	37.0
5	27.095120659383127	35.0	25.0	37.0	0.0	37.0
6	31.34780819613562	35.0	26.0	37.0	17.0	37.0
7	15.00102872220191	0.0	0.0	32.0	0.0	37.0
8	25.107117784521623	17.0	17.0	35.0	17.0	37.0
9	33.250335377339475	32.0	32.0	37.0	28.0	39.0
10	36.02479915589173	37.0	35.0	39.0	32.0	39.0
11	37.02266664257047	39.0	37.0	39.0	34.0	39.0
12	37.08940152000656	39.0	37.0	39.0	34.0	39.0
13	36.67989640489394	39.0	35.0	39.0	33.0	39.0
14	38.02367868279073	40.0	37.0	41.0	33.0	41.0
15	38.28254967407578	40.0	37.0	41.0	33.0	41.0
16	38.303524485673655	40.0	37.0	41.0	34.0	41.0
17	38.31167780420636	40.0	37.0	41.0	34.0	41.0
18	38.27240841939614	40.0	37.0	41.0	34.0	41.0
19	38.219153973300486	40.0	37.0	41.0	34.0	41.0
20	38.247258941889704	40.0	37.0	41.0	34.0	41.0
21	38.13204066511061	40.0	36.0	41.0	34.0	41.0
22	38.031912630901424	40.0	36.0	41.0	34.0	41.0
23	37.9284398593709	40.0	36.0	41.0	34.0	41.0
24	37.775575910662425	40.0	35.0	41.0	33.0	41.0
25	37.61143077186139	39.0	35.0	41.0	33.0	41.0
26	37.37582141382575	39.0	35.0	41.0	33.0	41.0
27	37.181145468270174	39.0	35.0	40.0	33.0	41.0
28	37.198009561555814	39.0	35.0	40.0	33.0	41.0
29	37.10411502782416	39.0	35.0	40.0	33.0	41.0
30	36.8877525061202	39.0	35.0	40.0	32.0	41.0
31	36.71266815785047	38.0	35.0	40.0	31.0	41.0
32	36.47079610586942	38.0	35.0	40.0	31.0	41.0
33	36.252629149816705	38.0	35.0	40.0	30.0	41.0
34	35.975334299961496	38.0	35.0	40.0	30.0	41.0
35	35.76416821554233	38.0	35.0	40.0	29.0	41.0
36	35.57507378301469	38.0	35.0	40.0	28.0	41.0
37	35.46921687827297	38.0	35.0	40.0	28.0	41.0
38	35.28602369675494	38.0	34.0	40.0	27.0	41.0
39	35.210183237666804	38.0	34.0	40.0	27.0	41.0
40	34.977109540842356	38.0	34.0	40.0	26.0	41.0
41	34.76342030669823	38.0	34.0	40.0	25.0	40.0
42	34.77437619814858	38.0	34.0	40.0	25.0	41.0
43	34.65066401237803	37.0	34.0	40.0	25.0	41.0
44	34.41673536399389	37.0	34.0	40.0	24.0	40.0
45	34.186223741518255	37.0	33.0	40.0	23.0	40.0
46	34.144638341588596	37.0	33.0	40.0	23.0	40.0
47	33.99258763948569	36.0	33.0	39.0	23.0	40.0
48	33.92418178355407	36.0	33.0	39.0	23.0	40.0
49	33.78312033686482	36.0	33.0	39.0	24.0	40.0
50	33.87912653144067	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	2.0
13	2.0
14	13.0
15	15.0
16	41.0
17	86.0
18	261.0
19	546.0
20	975.0
21	1673.0
22	2697.0
23	4152.0
24	6379.0
25	9476.0
26	11725.0
27	12521.0
28	12762.0
29	13842.0
30	17064.0
31	22216.0
32	30670.0
33	54368.0
34	57685.0
35	73059.0
36	100469.0
37	185030.0
38	80456.0
39	21150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.3420834960985	22.545142137434507	27.341350879070923	15.771423487396069
2	34.56798533097747	23.298611642076963	29.291752567287467	12.841650459658101
3	30.077612919638725	23.12456296683483	34.04027864470021	12.757545468826242
4	26.43468517625209	26.778751047836973	31.957811268400572	14.828752507510368
5	17.036473762718273	47.58215528422621	24.2793731467361	11.101997806319412
6	19.73019675007194	40.67261750023285	29.79735562787503	9.799830121820172
7	38.970638322126284	55.666799659131506	4.4179448076637025	0.944617211078504
8	86.66470189993869	2.7850568369016555	8.771803002478665	1.7784382606809863
9	82.68924665561022	4.30047585352664	10.26150396405589	2.7487735268072493
10	45.37832649140391	25.14461192845098	17.074147237950395	12.402914342194709
11	39.45358169096907	22.409323003479585	23.337675282446803	14.799420023104545
12	36.38145575312892	20.76003108409248	27.922578923150283	14.935934239628326
13	23.13971576683594	32.723931275796254	27.357615811182214	16.778737146185595
14	17.72682977010839	35.15546911817655	30.093599818722467	17.024101292992594
15	16.677255091132277	23.341984794373722	42.40184391503867	17.578916199455332
16	18.9614354289146	18.398001498598017	41.51616970579935	21.12439336668803
17	19.41323909867253	19.42213615555392	30.74433611968766	30.420288626085895
18	21.846306122704316	23.796012728352	33.711921639171514	20.645759509772166
19	27.641070482762647	25.401236412873484	26.505861631303183	20.451831473060686
20	28.8175672388123	23.574281388886188	26.543674123049076	21.064477249252437
21	24.12812317975252	25.920880141351994	29.626782365477194	20.32421431341829
22	22.89421260351517	23.850785234778037	27.902282512139614	25.352719649567174
23	21.10534810430131	28.336570101162316	27.791347334149823	22.76673446038655
24	21.110769748338402	24.276870849488212	36.537154248553186	18.0752051536202
25	21.153030768524992	24.018300133872902	32.877266490486406	21.9514026071157
26	18.7631978802762	29.461908780143993	28.7871226222963	22.987770717283507
27	19.056800757361966	28.631285110358263	30.904066094011306	21.407848038268465
28	17.962740793978917	26.57008726066569	35.99429476227481	19.472877183080577
29	18.998413821577863	24.612178680705483	35.174236347535725	21.215171150180932
30	20.83315377033638	26.874950475366973	32.67082696753547	19.621068786761178
31	27.28574427356226	25.634506122982348	27.055393910242596	20.024355693212797
32	27.554741227710437	25.851232867952383	26.945570864362978	19.6484550399742
33	26.795711062517118	25.644098262432596	26.272313888166778	21.287876786883515
34	20.578197484079134	26.483340956072173	29.47733961317265	23.461121946676045
35	21.2800918621123	25.102072875236846	30.45573783709767	23.16209742555318
36	27.32605906255604	25.89418897070783	26.462766512033966	20.316985454702163
37	21.43203691166474	30.64035176738645	26.98505155427413	20.942559766674684
38	22.0829122291437	30.315609191215824	25.67051140004921	21.930967179591264
39	21.54060880892041	28.80936526449977	26.02569859273583	23.624327333843986
40	23.8126947100046	24.91718091192053	28.115950893806673	23.154173484268195
41	20.576668302427645	24.203192097189223	28.471138086493298	26.749001513889837
42	22.388053476872518	26.806971400132625	25.032286585323472	25.77268853767139
43	22.12809259611949	26.382136934046397	25.9767647798882	25.51300568994591
44	21.416189029094763	27.920354658929934	28.346857323181418	22.316598988793878
45	20.394556669386755	31.273293954588866	25.256520222037178	23.0756291539872
46	22.17758247502221	29.45996254895119	27.654972134139815	20.707482841886787
47	22.126702430981776	27.69778922038149	27.949687143335755	22.225821205300978
48	22.277396331910268	25.85415221474159	29.42840580032502	22.440045653023123
49	21.679486306178312	25.73418096335664	30.18840908111475	22.397923649350307
50	20.423611120765038	29.01413658928544	28.831468890189466	21.730783399760057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	310.0
1	732.5
2	1155.0
3	6040.0
4	10925.0
5	7871.0
6	4817.0
7	4783.0
8	4749.0
9	4839.0
10	4929.0
11	4831.5
12	4734.0
13	4583.0
14	4432.0
15	4114.5
16	3797.0
17	3565.0
18	3333.0
19	3305.5
20	3278.0
21	3418.5
22	3559.0
23	3712.5
24	3866.0
25	4318.5
26	4771.0
27	5952.0
28	7133.0
29	8756.5
30	10380.0
31	12345.5
32	14311.0
33	16238.5
34	18166.0
35	20219.0
36	22272.0
37	25040.0
38	27808.0
39	34081.5
40	40355.0
41	46130.0
42	51905.0
43	54394.5
44	56884.0
45	57240.0
46	57596.0
47	57601.0
48	57606.0
49	54917.5
50	52229.0
51	47878.5
52	43528.0
53	40075.5
54	36623.0
55	35140.0
56	33657.0
57	33260.0
58	32863.0
59	30299.5
60	27736.0
61	24622.5
62	21509.0
63	18386.5
64	15264.0
65	13057.0
66	10850.0
67	9391.0
68	7932.0
69	6888.5
70	5845.0
71	4870.5
72	3896.0
73	3010.0
74	2124.0
75	1626.5
76	1129.0
77	848.0
78	567.0
79	438.5
80	310.0
81	214.0
82	118.0
83	74.5
84	31.0
85	26.5
86	22.0
87	20.5
88	19.0
89	12.0
90	5.0
91	5.0
92	5.0
93	4.5
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	719339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.510042772344732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.82600549836866	20.38864286060172
2	8.585551480051743	5.238910857610927
3	4.687111725137283	4.290119376380911
4	3.2340174828073374	3.9468004690785006
5	2.44846218315409	3.735134296724993
6	1.8995672909538575	3.4773527577569525
7	1.598962463013929	3.4149089196550055
8	1.3548314045584673	3.3068771281915788
9	1.1409874360705574	3.1330417929498595
>10	8.087197812627341	44.471583753889696
>50	0.11091916612348506	2.1160618434132665
>100	0.02443153438262617	1.3585790880078632
>500	4.886306876525235E-4	0.13581318437124584
>1k	0.0014658920629575703	0.9861736713674987
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3618	0.5029617468259054	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1540	0.21408543120837323	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1457	0.20254706056532457	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	911	0.12664404404599222	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.004587544954465141	0.0
2	0.0	0.0	0.0	0.015847882569970488	0.0
3	0.0	0.0	0.0	0.021964609175924008	0.0
4	0.0	0.0	0.0	0.03378101284651604	0.0
5	0.0	0.0	0.0	0.057552836701471766	0.0
6	0.0	0.0	0.0	0.11510567340294353	0.0
7	0.0	0.0	0.0	0.15750571010330316	0.0
8	0.0	0.0	0.0	0.40759641837853916	0.0
9	0.0	0.0	0.0	0.5210338936162227	0.0
10	0.0	0.0	0.0	0.6967507670236147	0.0
11	0.0	0.0	0.0	0.8409108918048375	0.0
12	0.0	0.0	0.0	0.9818736367693118	0.0
13	0.0	0.0	0.0	1.0352559780576334	0.0
14	0.0	0.0	0.0	1.0580546863161875	0.0
15	0.0	0.0	0.0	1.0946160294381369	0.0
16	0.0	0.0	0.0	1.1660705175167758	0.0
17	0.0	0.0	0.0	1.248368293669605	0.0
18	0.0	0.0	0.0	1.3819631634041807	0.0
19	0.0	0.0	0.0	1.4411841982709126	0.0
20	0.0	0.0	0.0	1.5054098276334247	0.0
21	0.0	0.0	0.0	1.5856223560796787	0.0
22	0.0	0.0	0.0	1.6709784955354847	0.0
23	0.0	0.0	0.0	1.7689851377445127	0.0
24	0.0	0.0	0.0	1.84530520380516	0.0
25	0.0	0.0	0.0	1.9025800074790884	0.0
26	0.0	0.0	0.0	1.9637472735386237	0.0
27	0.0	0.0	0.0	2.031587332259199	0.0
28	0.0	0.0	0.0	2.100400506576176	0.0
29	0.0	0.0	0.0	2.1801959854811153	0.0
30	0.0	0.0	0.0	2.2733370497081347	0.0
31	0.0	0.0	0.0	2.352993512099302	0.0
32	0.0	0.0	0.0	2.427089313939603	0.0
33	0.0	0.0	0.0	2.5067457763307703	0.0
34	0.0	0.0	0.0	2.601972088264365	0.0
35	0.0	0.0	0.0	2.71554858001582	0.0
36	0.0	0.0	0.0	2.806882429563808	0.0
37	0.0	0.0	0.0	2.902386774524946	0.0
38	0.0	0.0	0.0	3.008734407560274	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	40	8.305506E-9	44.0	6
ACACGAC	40	8.305506E-9	44.0	26
ACGCCCG	20	7.8545156E-4	44.0	27
CCGGATA	20	7.8545156E-4	44.0	20
CGATTAC	20	7.8545156E-4	44.0	10
CGTTCGA	40	8.305506E-9	44.0	14
TTATACG	20	7.8545156E-4	44.0	1
TCTACGG	30	2.5263907E-6	44.0	2
TTGCACG	25	4.441172E-5	44.0	1
CTAGGCG	40	8.305506E-9	44.0	5
CGTTTTA	1870	0.0	43.294117	1
CGGTCTA	140	0.0	42.428574	31
CGTTATT	1105	0.0	41.809956	1
CGAATAT	80	0.0	41.25	14
GTTATTT	1185	0.0	41.21519	2
GACGGTC	155	0.0	41.16129	29
TAAGGAC	285	0.0	40.91228	5
GTTTTAT	2150	0.0	40.827908	2
TTACTGG	985	0.0	40.64975	40
ACGGGTA	65	0.0	40.615387	5
>>END_MODULE