FastQCFastQC Report
Thu 26 May 2016
SRR1545771_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545771_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences277257
Sequences flagged as poor quality0
Sequence length51
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9560.34480644311956055No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8440.30441070919760366No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC3490.12587599231038352No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT207.024017E-445.0000046
GTGATAG302.1595242E-645.0000042
CAAGCGG551.8189894E-1245.00000421
CCAAGCG551.8189894E-1245.00000420
TAAGGCG302.1595242E-645.0000045
TTCATCG302.1595242E-645.00000415
CGTTTTA5600.045.0000041
TAGTACG207.024017E-445.0000041
TAGCCGT253.8831255E-545.044
CGGGACC253.8831255E-545.06
GGCCTTA502.1827873E-1145.024
AGTACGG253.8831255E-545.02
CATACGA650.044.99999618
ACATACG650.044.99999617
CGTTTTT6400.043.9453161
ATAGGAT950.042.631585
ACACCCA700.041.78571730
ATACGAA700.041.78571719
TATGCCA1250.041.410
GTAAGGC603.6379788E-1241.2500044
Produced by FastQC (version 0.11.5)