##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545771_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	277257
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.013557818197558	31.0	31.0	33.0	30.0	33.0
2	31.186177445474776	31.0	31.0	33.0	30.0	34.0
3	31.125378259160275	31.0	31.0	34.0	28.0	34.0
4	35.091842586481135	35.0	35.0	37.0	32.0	37.0
5	32.85214800708368	35.0	35.0	37.0	28.0	37.0
6	34.029193131282526	35.0	35.0	37.0	28.0	37.0
7	15.330992544823035	0.0	0.0	35.0	0.0	37.0
8	24.930479663272703	17.0	17.0	35.0	17.0	37.0
9	32.738185149518316	32.0	32.0	37.0	27.0	39.0
10	35.462386882928115	37.0	34.0	37.0	32.0	39.0
11	36.45065769304291	37.0	35.0	39.0	32.0	39.0
12	36.41007440749918	37.0	35.0	39.0	32.0	39.0
13	36.181730307981404	38.0	35.0	39.0	32.0	39.0
14	37.24309575592321	39.0	36.0	40.0	32.0	41.0
15	37.54990135506047	39.0	36.0	41.0	32.0	41.0
16	37.53568710618668	39.0	36.0	41.0	32.0	41.0
17	37.476572277706246	39.0	36.0	41.0	32.0	41.0
18	37.387373447739826	39.0	36.0	41.0	32.0	41.0
19	37.341549537072105	39.0	36.0	40.0	32.0	41.0
20	37.45843747858485	39.0	35.0	41.0	32.0	41.0
21	37.33612136032634	39.0	35.0	41.0	32.0	41.0
22	37.38677833201687	39.0	35.0	41.0	32.0	41.0
23	37.353513166484525	39.0	35.0	41.0	32.0	41.0
24	37.17748514915764	39.0	35.0	41.0	32.0	41.0
25	36.932820451783	39.0	35.0	40.0	31.0	41.0
26	36.83554608179415	39.0	35.0	40.0	31.0	41.0
27	36.759490292400194	39.0	35.0	40.0	31.0	41.0
28	36.656394608612224	38.0	35.0	40.0	31.0	41.0
29	36.59709944203392	38.0	35.0	40.0	31.0	41.0
30	36.31674583509163	38.0	35.0	40.0	30.0	41.0
31	36.19499958522238	38.0	35.0	40.0	30.0	41.0
32	36.09513195338621	38.0	35.0	40.0	30.0	41.0
33	35.90461557327678	38.0	35.0	40.0	29.0	41.0
34	35.68582217942198	38.0	35.0	40.0	27.0	41.0
35	35.4941444219623	38.0	34.0	40.0	27.0	41.0
36	35.28009031331941	38.0	34.0	40.0	25.0	41.0
37	35.16571628489091	38.0	34.0	40.0	25.0	41.0
38	35.04696364744623	38.0	34.0	40.0	25.0	41.0
39	34.9357275019206	38.0	34.0	40.0	24.0	41.0
40	34.7675009107074	38.0	34.0	40.0	24.0	41.0
41	34.60697836303502	38.0	34.0	40.0	23.0	41.0
42	34.558351998326465	38.0	33.0	40.0	23.0	41.0
43	34.49538514807561	38.0	33.0	40.0	23.0	41.0
44	34.171533991928065	37.0	33.0	40.0	23.0	41.0
45	34.12991556570258	37.0	33.0	40.0	23.0	41.0
46	34.09661072578871	37.0	33.0	40.0	23.0	41.0
47	34.053059075154096	37.0	33.0	40.0	23.0	41.0
48	34.00711614134179	37.0	33.0	40.0	23.0	41.0
49	33.90581301824661	36.0	33.0	40.0	22.0	41.0
50	33.79718456161612	36.0	33.0	40.0	22.0	41.0
51	33.63978907656074	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	6.0
15	16.0
16	31.0
17	73.0
18	205.0
19	327.0
20	597.0
21	966.0
22	1483.0
23	2120.0
24	3101.0
25	4204.0
26	4992.0
27	5321.0
28	5733.0
29	6363.0
30	7749.0
31	9929.0
32	13313.0
33	19077.0
34	24678.0
35	27313.0
36	34018.0
37	50886.0
38	45006.0
39	9747.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.894184817696214	23.215284014470328	27.67973396523803	15.210797202595424
2	34.290207280609685	23.24630216730326	29.53721637325658	12.926274178830472
3	30.77397504842078	22.64325156803976	33.70591184352424	12.87686154001522
4	27.055042794230623	27.59353235445814	30.398871804859752	14.952553046451486
5	20.737799225988883	35.97240105750261	29.4113403809462	13.878459335562313
6	19.786335421648506	41.33637744042531	29.13975120556018	9.737535932365999
7	38.083078154924856	56.63049084423477	4.580587685793326	0.7058433150470502
8	87.45892799821104	2.980988757722979	7.876086086194397	1.6839971578715776
9	83.20547362194642	4.53442113273966	9.431682518385468	2.8284227269284457
10	45.246468078353296	25.98960531203901	16.459458192218772	12.304468417388922
11	39.60404967232567	23.03133915464713	22.730535207406845	14.634075965620344
12	36.540826741975856	21.151494822493213	27.382176103759328	14.925502331771606
13	23.358472464175836	33.60744724208947	26.312410507218935	16.72166978651576
14	17.570701551268318	36.07375106850323	29.357960303977897	16.997587076250554
15	16.69930786238039	23.84177856645639	41.84781628597295	17.611097285190276
16	18.998257933974617	18.856512189052037	40.93602686316306	21.209203013810292
17	19.230533404025866	19.917982233090598	30.256765383741442	30.594718979142094
18	22.07410453117505	24.154124151960094	33.24604969396624	20.52572162289861
19	27.892893596915496	25.752280375247516	25.807463833194472	20.547362194642517
20	29.311433074728498	23.95142412995885	25.756969165792025	20.98017362952063
21	24.228062772085103	26.63774043576898	28.835701172558316	20.2984956195876
22	22.90582383853248	24.11625315140826	27.093635147173924	25.884287862885337
23	21.097393393133444	28.767894047760738	27.20941220600381	22.925300353101996
24	20.90082486645964	24.678547340554072	36.31107600529473	18.109551787691565
25	21.122640726834668	24.40371207940647	32.51784445478383	21.95580273897503
26	18.643352557374566	30.228993316670095	28.188287401219807	22.939366724735535
27	18.86985720829411	29.49213184879011	30.33539279441096	21.302618148504816
28	17.980790385815325	27.210854910786743	35.473585878805586	19.334768824592345
29	19.015570391369742	25.213430138824265	34.56215713219144	21.20884233761456
30	20.81498393187548	27.637895526533146	32.10847697262829	19.438643568963094
31	27.525003877269107	25.997179512149376	26.527734196070796	19.950082414510725
32	27.935453388011844	26.00619641704267	26.589770501736652	19.468579693208827
33	27.320139798093468	25.919273453871316	25.756969165792025	21.00361758224319
34	20.569724118777884	26.656495597947032	28.85409565854063	23.91968462473445
35	21.332554272750553	24.99882780236387	30.37759190931158	23.291026015573998
36	27.6566506887112	26.333690402767107	25.920716158654244	20.088942749867453
37	21.370425273302388	31.460341848898317	26.612132425872026	20.557100451927273
38	21.878257356892703	30.86594747833238	25.354454531355387	21.901340633419537
39	21.310553024810915	29.517379182491332	25.76129728014081	23.41077051255694
40	24.028608835845443	25.000991859538264	27.988472788784414	22.981926515831884
41	20.349711639381514	24.49784856649246	28.380888489740563	26.77155130438546
42	22.116664322271397	26.853785477012305	24.980793992577283	26.048756208139018
43	21.698640611418288	26.5641624918397	25.951012959095713	25.7861839376463
44	21.13490371748955	28.206321211006397	28.36285467995398	22.295920391550077
45	20.343580144054073	31.804787615822143	25.116408242172422	22.73522399795136
46	21.976000605936008	29.963896312807254	27.41283358039653	20.64726950086021
47	22.04597178790797	28.152941134038095	27.346108484186153	22.454978593867782
48	22.08672819802566	26.253620287314654	29.486361029658404	22.173290485001278
49	21.772579231543297	26.124498209242685	30.215648297428015	21.887274261785997
50	20.36954883014676	29.685454289702335	28.557619825649127	21.38737705450178
51	19.734758725658867	30.313030870275593	27.36666702734286	22.58554337672268
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	281.5
2	419.0
3	1954.5
4	3490.0
5	2540.5
6	1591.0
7	1580.0
8	1569.0
9	1594.5
10	1620.0
11	1595.0
12	1570.0
13	1529.0
14	1488.0
15	1396.0
16	1304.0
17	1205.5
18	1107.0
19	1123.5
20	1140.0
21	1222.0
22	1304.0
23	1369.5
24	1435.0
25	1594.5
26	2087.0
27	2420.0
28	3106.0
29	3792.0
30	4455.0
31	5118.0
32	5987.5
33	6857.0
34	7678.5
35	8500.0
36	9434.0
37	10368.0
38	12251.5
39	14135.0
40	16529.5
41	18924.0
42	19946.5
43	20969.0
44	21474.5
45	21980.0
46	21861.5
47	21743.0
48	20907.5
49	20072.0
50	19162.5
51	18253.0
52	16888.5
53	15524.0
54	14489.0
55	13454.0
56	12862.0
57	12270.0
58	11811.5
59	11353.0
60	10177.0
61	9001.0
62	7875.0
63	6749.0
64	5855.0
65	4961.0
66	4225.5
67	3490.0
68	3029.5
69	2569.0
70	2237.5
71	1906.0
72	1646.5
73	1387.0
74	1071.0
75	557.0
76	359.0
77	284.5
78	210.0
79	156.5
80	103.0
81	79.0
82	55.0
83	35.0
84	15.0
85	12.0
86	9.0
87	9.5
88	10.0
89	9.0
90	8.0
91	5.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.15199166918508
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.97301438528564	28.900189588140584
2	10.87518364558912	9.385716681506391
3	5.966815007650487	7.72439854505107
4	3.994872398150491	6.895468017777897
5	2.780806526945822	5.999867004219081
6	2.0211901846326334	5.233102920546364
7	1.6035480422626858	4.843740423260023
8	1.2095096659264675	4.175420082228619
9	1.0292024425621085	3.9970921704610545
>10	3.514973351570463	21.008010495406936
>50	0.020867803664922815	0.6494314334779997
>100	0.008347121465969125	0.53921178919388
>500	0.0016694242931938252	0.6483508487301163
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	956	0.34480644311956055	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	844	0.30441070919760366	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	349	0.12587599231038352	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.003246085761585821	0.0
2	0.0	0.0	0.0	0.01695178119939262	0.0
3	0.0	0.0	0.0	0.02200124793963723	0.0
4	0.0	0.0	0.0	0.0317395052243947	0.0
5	0.0	0.0	0.0	0.054101429359763684	0.0
6	0.0	0.0	0.0	0.0890870203457442	0.0
7	0.0	0.0	0.0	0.11794111600428483	0.0
8	0.0	0.0	0.0	0.3195591094183375	0.0
9	0.0	0.0	0.0	0.4104495107427405	0.0
10	0.0	0.0	0.0	0.5514739032738578	0.0
11	0.0	0.0	0.0	0.6676116382994839	0.0
12	0.0	0.0	0.0	0.7635515063641315	0.0
13	0.0	0.0	0.0	0.8140461737665776	0.0
14	0.0	0.0	0.0	0.8349653931190195	0.0
15	0.0	0.0	0.0	0.8591306982330473	0.0
16	0.0	0.0	0.0	0.9211670038989097	0.0
17	0.0	0.0	0.0	0.9929415668495295	0.0
18	0.0	0.0	0.0	1.1170141781812541	0.0
19	0.0	0.0	0.0	1.166426816996505	0.0
20	0.0	0.0	0.0	1.220167570160537	0.0
21	0.0	0.0	0.0	1.2915814569154251	0.0
22	0.0	0.0	0.0	1.364798724648972	0.0
23	0.0	0.0	0.0	1.4535250687989842	0.0
24	0.0	0.0	0.0	1.513757993486188	0.0
25	0.0	0.0	0.0	1.5660560418672929	0.0
26	0.0	0.0	0.0	1.62701031894596	0.0
27	0.0	0.0	0.0	1.6858005388502364	0.0
28	0.0	0.0	0.0	1.735573853861219	0.0
29	0.0	0.0	0.0	1.802659626267326	0.0
30	0.0	0.0	0.0	1.8946320561789243	0.0
31	0.0	0.0	0.0	1.9638818857594218	0.0
32	0.0	0.0	0.0	2.0316890105569922	0.0
33	0.0	0.0	0.0	2.106709659269198	0.0
34	0.0	0.0	0.0	2.1831730127643305	0.0
35	0.0	0.0	0.0	2.2805555856119053	0.0
36	0.0	0.0	0.0	2.368560577370454	0.0
37	0.0	0.0	0.0	2.447909340431441	0.0
38	0.0	0.0	0.0	2.537357036972917	0.0
39	0.0	0.0	0.0	2.6729712865680577	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	20	7.024017E-4	45.000004	6
GTGATAG	30	2.1595242E-6	45.000004	2
CAAGCGG	55	1.8189894E-12	45.000004	21
CCAAGCG	55	1.8189894E-12	45.000004	20
TAAGGCG	30	2.1595242E-6	45.000004	5
TTCATCG	30	2.1595242E-6	45.000004	15
CGTTTTA	560	0.0	45.000004	1
TAGTACG	20	7.024017E-4	45.000004	1
TAGCCGT	25	3.8831255E-5	45.0	44
CGGGACC	25	3.8831255E-5	45.0	6
GGCCTTA	50	2.1827873E-11	45.0	24
AGTACGG	25	3.8831255E-5	45.0	2
CATACGA	65	0.0	44.999996	18
ACATACG	65	0.0	44.999996	17
CGTTTTT	640	0.0	43.945316	1
ATAGGAT	95	0.0	42.63158	5
ACACCCA	70	0.0	41.785717	30
ATACGAA	70	0.0	41.785717	19
TATGCCA	125	0.0	41.4	10
GTAAGGC	60	3.6379788E-12	41.250004	4
>>END_MODULE