FastQCFastQC Report
Thu 26 May 2016
SRR1545770_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545770_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences960480
Sequences flagged as poor quality0
Sequence length50
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36020.3750208229218724No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25760.2681992337164751No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14900.15513076794935868No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.14857154755955354No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA207.8558363E-444.013
AGCCGAA207.8558363E-444.037
TCGTTAA351.445751E-744.01
GTCGATA207.8558363E-444.01
TTTACGG502.7284841E-1144.02
GCGAATA254.4422908E-544.01
ACGTTAC207.8558363E-444.023
CGGTTGA302.5272802E-644.029
CGCATCG454.802132E-1044.021
TATGCGC254.4422908E-544.011
GTAATCG302.5272802E-644.028
GTTACGG1100.044.02
CTAACGG351.445751E-744.02
GCCGAAC207.8558363E-444.041
CGTAGAT207.8558363E-444.023
TAACACG207.8558363E-444.01
CGCTAAG351.445751E-744.02
TACCTCG207.8558363E-444.025
TTGCGAA502.7284841E-1144.01
ACGCTAG408.310963E-944.02
Produced by FastQC (version 0.11.5)