##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545770_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	960480
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56036877394636	31.0	31.0	33.0	30.0	34.0
2	31.74580418124271	31.0	31.0	34.0	30.0	34.0
3	31.792993086789938	31.0	31.0	34.0	30.0	34.0
4	35.688968015992	37.0	35.0	37.0	35.0	37.0
5	27.128182783608196	35.0	25.0	37.0	0.0	37.0
6	31.35982217224721	35.0	26.0	37.0	17.0	37.0
7	14.972244086290189	0.0	0.0	32.0	0.0	37.0
8	25.083801849075464	17.0	17.0	35.0	17.0	37.0
9	33.20914646843245	32.0	32.0	37.0	28.0	39.0
10	36.118277319673496	37.0	35.0	39.0	32.0	39.0
11	37.09588643178411	39.0	37.0	39.0	34.0	39.0
12	36.926318090954524	39.0	35.0	39.0	33.0	39.0
13	36.34079314509412	39.0	35.0	39.0	32.0	39.0
14	37.65198234216225	40.0	36.0	41.0	33.0	41.0
15	37.97886889888389	40.0	36.0	41.0	33.0	41.0
16	38.07080730468099	40.0	36.0	41.0	33.0	41.0
17	38.10891325170748	40.0	36.0	41.0	34.0	41.0
18	38.077894386140265	40.0	36.0	41.0	34.0	41.0
19	38.01242191404298	40.0	36.0	41.0	34.0	41.0
20	38.01854593536565	40.0	36.0	41.0	34.0	41.0
21	37.86050516408462	40.0	35.0	41.0	34.0	41.0
22	37.77821714142929	40.0	35.0	41.0	33.0	41.0
23	37.689000291520905	39.0	35.0	41.0	33.0	41.0
24	37.54176349325337	39.0	35.0	41.0	33.0	41.0
25	37.36275299850075	39.0	35.0	41.0	33.0	41.0
26	37.11129747626187	39.0	35.0	40.0	33.0	41.0
27	36.91250728802265	38.0	35.0	40.0	32.0	41.0
28	36.956248958853905	39.0	35.0	40.0	32.0	41.0
29	36.94575941196069	38.0	35.0	40.0	33.0	41.0
30	36.76559636848243	38.0	35.0	40.0	32.0	41.0
31	36.55428431617525	38.0	35.0	40.0	32.0	41.0
32	36.291004497751125	38.0	35.0	40.0	31.0	41.0
33	36.105163043478264	38.0	35.0	40.0	31.0	41.0
34	35.88892116441779	38.0	35.0	40.0	30.0	41.0
35	35.72549766783275	38.0	35.0	40.0	30.0	41.0
36	35.53617149758454	38.0	35.0	40.0	29.0	41.0
37	35.36928306679993	38.0	34.0	40.0	29.0	41.0
38	35.21076857404631	38.0	34.0	40.0	28.0	41.0
39	35.18496064467766	38.0	34.0	40.0	28.0	41.0
40	34.94106384307846	37.0	34.0	40.0	27.0	41.0
41	34.78288564051308	37.0	34.0	40.0	26.0	40.0
42	34.769760952856906	37.0	34.0	40.0	26.0	41.0
43	34.6506361402632	37.0	34.0	40.0	26.0	41.0
44	34.43499500249875	37.0	34.0	40.0	25.0	40.0
45	34.22492191404298	36.0	33.0	40.0	24.0	40.0
46	34.21774945860403	36.0	33.0	39.0	24.0	40.0
47	34.07177973513243	36.0	33.0	39.0	24.0	40.0
48	34.021133183408296	36.0	33.0	39.0	24.0	40.0
49	33.93352802765284	36.0	33.0	39.0	24.0	40.0
50	33.93307304680993	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	0.0
14	5.0
15	11.0
16	45.0
17	142.0
18	355.0
19	698.0
20	1303.0
21	2167.0
22	3466.0
23	5298.0
24	7888.0
25	11770.0
26	14717.0
27	16346.0
28	17126.0
29	19229.0
30	23500.0
31	31688.0
32	44392.0
33	87907.0
34	84968.0
35	100903.0
36	122938.0
37	231509.0
38	107203.0
39	24903.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.5994294519407	24.094202898550723	28.512826919873397	14.793540729635183
2	33.13426620023321	24.511181909045476	29.69837997667833	12.656171914042979
3	30.51120273196735	23.235361485923704	33.52709062135599	12.726345160752958
4	26.407941862402133	26.37264700982842	32.132579543561555	15.086831584207896
5	17.11769115442279	48.763118440779614	23.28492003998001	10.834270364817591
6	20.58179243711478	40.77450857904381	28.745731301016157	9.897967682825254
7	38.935844577711144	56.06530068299184	3.958333333333333	1.040521405963685
8	87.24897967682826	3.4497334666000334	7.6164001332667	1.6848867233050142
9	83.03889721805764	5.060907046476762	9.23871397634516	2.6614817591204396
10	54.819361152756954	20.735882058970514	13.999354489421956	10.445402298850574
11	50.641658337497915	18.387993503248374	18.987693653173412	11.982654506080294
12	44.2407962685324	20.785336498417458	22.676890721306016	12.296976511744127
13	22.011598367482925	41.98567382975179	23.147280526403467	12.85544727636182
14	15.038001832417125	44.018095119107116	27.706667499583542	13.23723554889222
15	14.192278860569715	21.961727469598536	50.0668415792104	13.779152090621356
16	15.61864900882892	15.656754955855407	49.636327669498584	19.08826836581709
17	16.182846076961518	15.930368149258703	30.156692487089785	37.730093286689986
18	21.315696318507413	22.707292187239712	35.3404547726137	20.63655672163918
19	30.53317091454273	22.494898384141262	26.443861402632017	20.52806929868399
20	32.838893053473264	22.23575712143928	24.124604364484426	20.800745460603032
21	23.06096951524238	27.389326170248207	28.158733133433284	21.39097118107613
22	22.99922955189072	24.06890304847576	25.04758037647843	27.884287023155093
23	20.050495585540563	29.55418124271198	24.9695985340663	25.425724637681157
24	20.567320506413463	23.10251124437781	39.59676411794103	16.733404131267697
25	18.147905214059637	23.24462768615692	37.25033316674996	21.357133933033484
26	16.23063468265867	33.53333749791771	29.015804597701152	21.220223221722474
27	18.19694319506913	32.314571880726305	30.715475595535562	18.773009328668998
28	15.079751790771281	27.76424287856072	40.70506413459937	16.450941196068634
29	16.234903381642514	24.492441279360317	38.36665000832917	20.906005330668
30	19.15323588205897	29.292645343994668	32.142053973013496	19.412064800932864
31	30.379810094952525	25.160336498417458	25.01686656671664	19.442986839913377
32	30.13826420123272	25.539417791104448	27.480738797268035	16.8415792103948
33	29.033816425120772	26.08175079127103	25.417083125104117	19.467349658504084
34	19.782712810261536	26.978281692487087	28.559886723305016	24.67911877394636
35	20.277257204730965	24.39030484757621	32.04585207396302	23.286585873729802
36	31.48488255872064	23.648384141262703	26.113401632517075	18.753331667499584
37	20.939322005663836	31.998479926703315	28.534482758620687	18.52771530901216
38	21.04853823088456	32.81192736964851	23.766450108279194	22.373084291187737
39	22.152569548559054	30.260598867233053	26.40315259037148	21.183678993836416
40	23.94854656005331	25.373667333000167	25.472472097284694	25.205314009661834
41	18.037127269698484	23.45847909378644	27.610465600533068	30.89392803598201
42	22.21097784441113	26.576295185740463	23.232342162252205	27.980384807596202
43	22.948005164084627	26.082063135099116	24.837164750957854	26.132766949858404
44	19.999687656171915	30.325878727303014	28.233175079127104	21.441258537397967
45	17.76476345160753	37.11508828918874	22.907088122605366	22.21306013659837
46	22.906567549558552	31.475095785440615	26.343911377644513	19.274425287356323
47	22.783087622855238	26.599720972846907	26.779214559386972	23.837976844910877
48	23.58581126103615	23.561344327836082	30.459145427286355	22.39369898384141
49	21.140783774779276	23.14603115109112	32.46189405297351	23.25129102115609
50	19.637472930201564	32.294373646510074	27.22586623354989	20.842287189738464
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	793.0
1	1156.5
2	1520.0
3	6259.0
4	10998.0
5	8182.0
6	5366.0
7	5567.5
8	5769.0
9	6078.0
10	6387.0
11	6389.0
12	6391.0
13	5927.0
14	5463.0
15	5161.5
16	4860.0
17	4526.5
18	4193.0
19	4044.0
20	3895.0
21	4169.5
22	4444.0
23	4856.0
24	5268.0
25	6019.5
26	6771.0
27	7228.0
28	7685.0
29	9235.0
30	10785.0
31	13158.0
32	15531.0
33	16706.5
34	17882.0
35	19011.5
36	20141.0
37	26019.0
38	31897.0
39	45679.0
40	59461.0
41	75207.0
42	90953.0
43	95044.0
44	99135.0
45	95530.0
46	91925.0
47	85855.5
48	79786.0
49	76629.0
50	73472.0
51	68768.0
52	64064.0
53	56166.0
54	48268.0
55	43229.0
56	38190.0
57	36849.0
58	35508.0
59	33885.5
60	32263.0
61	28317.5
62	24372.0
63	20306.0
64	16240.0
65	13627.0
66	11014.0
67	9309.0
68	7604.0
69	6502.0
70	5400.0
71	4425.0
72	3450.0
73	2673.0
74	1896.0
75	1331.0
76	766.0
77	544.5
78	323.0
79	269.5
80	216.0
81	152.5
82	89.0
83	55.0
84	21.0
85	15.5
86	10.0
87	8.5
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	960480.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.520103898813634
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.85326220968282	14.744364060113908
2	6.254487069943733	2.566854490180637
3	2.789889082323395	1.7174644150632565
4	1.8683721676835878	1.5335676401007545
5	1.4528754603774099	1.4906577699490566
6	1.1412553817543565	1.4051207405207766
7	0.9788301976396624	1.4059988148403506
8	0.890832608803252	1.4623982151275143
9	0.7700644460484323	1.4221622197526576
>10	10.41930865263984	47.84683735222408
>50	1.4418955596885357	19.208559656878204
>100	0.13629596713823114	4.153097021635766
>500	5.262392553599658E-4	0.0607954660213452
>1k	0.002104957021439863	0.9821221375917132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3602	0.3750208229218724	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2576	0.2681992337164751	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1490	0.15513076794935868	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1427	0.14857154755955354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008953856405130767	0.0
2	0.0	0.0	0.0	0.029672663668165917	0.0
3	0.0	0.0	0.0	0.03768948858903881	0.0
4	0.0	0.0	0.0	0.052994336165250705	0.0
5	0.0	0.0	0.0	0.08828918873896385	0.0
6	0.0	0.0	0.0	0.14784274529401967	0.0
7	0.0	0.0	0.0	0.18584457771114443	0.0
8	0.0	0.0	0.0	0.40667166416791606	0.0
9	0.0	0.0	0.0	0.5080792936864901	0.0
10	0.0	0.0	0.0	0.6719556888222555	0.0
11	0.0	0.0	0.0	0.8096993170081626	0.0
12	0.0	0.0	0.0	0.9483799766783275	0.0
13	0.0	0.0	0.0	0.9996043644844245	0.0
14	0.0	0.0	0.0	1.0160544727636183	0.0
15	0.0	0.0	0.0	1.0443736465100784	0.0
16	0.0	0.0	0.0	1.1149633516575046	0.0
17	0.0	0.0	0.0	1.2029401965683826	0.0
18	0.0	0.0	0.0	1.344536065300683	0.0
19	0.0	0.0	0.0	1.3987797767782775	0.0
20	0.0	0.0	0.0	1.459895052473763	0.0
21	0.0	0.0	0.0	1.5290271530901216	0.0
22	0.0	0.0	0.0	1.6005538897218057	0.0
23	0.0	0.0	0.0	1.685615525570548	0.0
24	0.0	0.0	0.0	1.7472513743128435	0.0
25	0.0	0.0	0.0	1.7974346160253207	0.0
26	0.0	0.0	0.0	1.853656505080793	0.0
27	0.0	0.0	0.0	1.9057138097617858	0.0
28	0.0	0.0	0.0	1.9565217391304348	0.0
29	0.0	0.0	0.0	2.012639513576545	0.0
30	0.0	0.0	0.0	2.090829585207396	0.0
31	0.0	0.0	0.0	2.1676661669165416	0.0
32	0.0	0.0	0.0	2.230863734799267	0.0
33	0.0	0.0	0.0	2.3003081792437117	0.0
34	0.0	0.0	0.0	2.3745418957188074	0.0
35	0.0	0.0	0.0	2.470223221722472	0.0
36	0.0	0.0	0.0	2.5483091787439616	0.0
37	0.0	0.0	0.0	2.63003914709312	0.0
38	0.0	0.0	0.0	2.7079168748958855	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	20	7.8558363E-4	44.0	13
AGCCGAA	20	7.8558363E-4	44.0	37
TCGTTAA	35	1.445751E-7	44.0	1
GTCGATA	20	7.8558363E-4	44.0	1
TTTACGG	50	2.7284841E-11	44.0	2
GCGAATA	25	4.4422908E-5	44.0	1
ACGTTAC	20	7.8558363E-4	44.0	23
CGGTTGA	30	2.5272802E-6	44.0	29
CGCATCG	45	4.802132E-10	44.0	21
TATGCGC	25	4.4422908E-5	44.0	11
GTAATCG	30	2.5272802E-6	44.0	28
GTTACGG	110	0.0	44.0	2
CTAACGG	35	1.445751E-7	44.0	2
GCCGAAC	20	7.8558363E-4	44.0	41
CGTAGAT	20	7.8558363E-4	44.0	23
TAACACG	20	7.8558363E-4	44.0	1
CGCTAAG	35	1.445751E-7	44.0	2
TACCTCG	20	7.8558363E-4	44.0	25
TTGCGAA	50	2.7284841E-11	44.0	1
ACGCTAG	40	8.310963E-9	44.0	2
>>END_MODULE