FastQCFastQC Report
Thu 26 May 2016
SRR1545768_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545768_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1385328
Sequences flagged as poor quality0
Sequence length50
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51200.3695875633784923No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21990.15873497106822357No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20000.14437014194472356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAC207.857042E-444.00000413
CGCCTAT207.857042E-444.00000430
CGTAAAT408.314601E-944.00000416
CGTTATT19000.042.7263151
CGTTTTA33650.042.6924251
AGGCGAT11950.042.3430987
AGTACGG1050.041.904762
TTACGGG3850.041.7142873
TTGCGAG900.041.5555572
CACGACG1850.040.4324326
GCGCAAA603.6379788E-1240.3333321
CGGTCTA1800.040.33333231
ACGCATT501.3478711E-939.60000229
CGAAGGA5000.039.64
CGACGGT1950.039.4871828
ACGGTCT1850.039.2432430
TACGGGA3900.038.9230774
GCGAATA404.1253588E-738.5000041
GCTAACG404.1253588E-738.5000041
TAGGCGA3600.038.56