##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545768_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1385328
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.58654701269302	31.0	31.0	33.0	30.0	34.0
2	31.776081909843732	31.0	31.0	34.0	30.0	34.0
3	31.816780574708662	31.0	31.0	34.0	30.0	34.0
4	35.713028972200085	37.0	35.0	37.0	35.0	37.0
5	27.204808536317753	35.0	26.0	37.0	0.0	37.0
6	31.414477293464074	36.0	28.0	37.0	17.0	37.0
7	14.927178978552371	0.0	0.0	32.0	0.0	37.0
8	25.082830203388657	17.0	17.0	35.0	17.0	37.0
9	33.26472792003049	32.0	32.0	37.0	28.0	39.0
10	36.06552599817516	37.0	35.0	39.0	32.0	39.0
11	37.002595775152166	39.0	37.0	39.0	34.0	39.0
12	37.16367459547486	39.0	37.0	39.0	34.0	39.0
13	36.84296643105459	39.0	35.0	39.0	33.0	39.0
14	38.21904704156705	40.0	37.0	41.0	33.0	41.0
15	38.431252382107346	40.0	37.0	41.0	34.0	41.0
16	38.44430199923772	40.0	37.0	41.0	34.0	41.0
17	38.44888575124447	40.0	37.0	41.0	34.0	41.0
18	38.40740820946375	40.0	37.0	41.0	34.0	41.0
19	38.374673723479205	40.0	37.0	41.0	34.0	41.0
20	38.41582137948558	40.0	37.0	41.0	34.0	41.0
21	38.30663857223704	40.0	37.0	41.0	34.0	41.0
22	38.218909889932206	40.0	37.0	41.0	34.0	41.0
23	38.114560595035975	40.0	36.0	41.0	34.0	41.0
24	37.99231878659783	40.0	36.0	41.0	34.0	41.0
25	37.834374963907464	40.0	35.0	41.0	33.0	41.0
26	37.61083801092593	39.0	35.0	41.0	33.0	41.0
27	37.41807860665489	39.0	35.0	40.0	33.0	41.0
28	37.45147358603883	39.0	35.0	40.0	33.0	41.0
29	37.4187405437557	39.0	35.0	40.0	33.0	41.0
30	37.18782483281937	39.0	35.0	40.0	33.0	41.0
31	37.03046426550247	39.0	35.0	40.0	32.0	41.0
32	36.83836752018295	39.0	35.0	40.0	32.0	41.0
33	36.640654776341776	39.0	35.0	40.0	31.0	41.0
34	36.418272062645094	39.0	35.0	40.0	31.0	41.0
35	36.23392077544091	39.0	35.0	40.0	30.0	41.0
36	36.08962426226857	38.0	35.0	40.0	30.0	41.0
37	35.955459645657925	38.0	35.0	40.0	30.0	41.0
38	35.798468665904394	38.0	35.0	40.0	30.0	41.0
39	35.73667391404779	38.0	35.0	40.0	29.0	41.0
40	35.538094227504246	38.0	35.0	40.0	29.0	41.0
41	35.337116552903	38.0	34.0	40.0	28.0	41.0
42	35.35268976011457	38.0	35.0	40.0	28.0	41.0
43	35.2407278276336	38.0	34.0	40.0	27.0	41.0
44	35.02496448494508	38.0	34.0	40.0	27.0	40.0
45	34.8516452421376	38.0	34.0	40.0	27.0	40.0
46	34.767752474504235	37.0	34.0	40.0	26.0	40.0
47	34.616866186202834	37.0	34.0	40.0	26.0	40.0
48	34.51099162075696	37.0	34.0	39.0	26.0	40.0
49	34.387572473811254	37.0	34.0	39.0	26.0	40.0
50	34.52684201864107	37.0	34.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	6.0
14	10.0
15	19.0
16	46.0
17	149.0
18	371.0
19	758.0
20	1460.0
21	2413.0
22	4144.0
23	6505.0
24	10364.0
25	15408.0
26	19113.0
27	20416.0
28	20858.0
29	23117.0
30	29047.0
31	38929.0
32	54728.0
33	95409.0
34	106541.0
35	138363.0
36	198812.0
37	387474.0
38	165052.0
39	45814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.46471016250303	22.697801531478465	28.582617257429288	15.254871048589214
2	34.13985713130753	24.269342711617753	28.995154938036332	12.59564521903838
3	30.09475012415832	23.295349548987677	34.002705496460045	12.607194830393958
4	26.601209244308926	27.61800815402562	31.58270099211162	14.198081609553839
5	17.271433191273115	47.77568922305765	23.858032177217236	11.094845408452006
6	20.059220632225724	41.120081309263945	28.68959553261033	10.131102525900003
7	39.342451751498565	55.84655763833547	3.9148851391150687	0.8961054710508991
8	88.5204081632653	2.486486954713974	7.595457537853852	1.3976473441668686
9	83.92250788260975	4.804060843352621	8.898975549472759	2.3744557245648683
10	42.66801797119527	32.46920584872319	14.548901054479515	10.313875125602024
11	33.77748807502628	23.303650832149497	28.144237321414135	14.774623771410091
12	31.348171696522414	23.46238580321772	29.950885277710405	15.238557222549462
13	21.44199785177229	33.2801329360267	29.97456198098934	15.303307231211669
14	17.109233336798216	36.1640708915145	30.74484887333541	15.98184689835187
15	16.114739614011988	25.54225425314438	42.8217721723664	15.521233960477229
16	18.480172204705312	22.779081921393345	41.146717600452746	17.594028273448597
17	19.219419516533268	21.693201898756108	31.72122414330758	27.366154441403047
18	21.487691001697794	24.523650716653382	34.560118614508625	19.428539667140203
19	26.2176177771618	25.163354815610457	29.141546261968287	19.47748114525946
20	27.880400887010154	25.01674693646559	28.690750493745888	18.412101682778374
21	23.21753404247947	26.437349133201664	31.369033182033423	18.976083642285438
22	22.659254703579222	24.21910190222099	30.144557823129254	22.977085571070532
23	19.44593626924454	29.249535128142938	29.74140420174861	21.563124400863913
24	19.32466535001097	25.807101278541978	36.68553584421884	18.182697527228207
25	18.683228809350567	26.65188316413153	34.977276139657896	19.687611886860008
26	18.10971842047515	31.558230253051985	29.902376910016976	20.429674416455885
27	18.461981566820278	30.775455343427694	32.293290830763546	18.469272258988482
28	17.351558620052433	27.442237506207917	36.989651548225396	18.216552325514247
29	18.07362588498897	25.74624926371228	36.15129413395239	20.02883071734636
30	19.618819514223347	28.520393726251115	33.11244701615791	18.748339743367637
31	25.610469145213266	27.068102283358165	28.451384798401534	18.87004377302704
32	25.619636649226752	26.6731055749974	29.701558042571868	18.00569973320398
33	24.840687548364	26.989637111211206	28.718902671425106	19.45077266899969
34	20.249211739024982	27.47558700899715	30.685007449499324	21.590193802478545
35	20.25303754778652	26.367618354642364	32.14134125636672	21.23800284120439
36	25.84420440502177	26.359894552048324	29.039260016400448	18.756641026529458
37	21.1489264636245	30.08796472748692	29.668786020350414	19.094322788538165
38	20.501065451647552	31.34867699201922	27.575779887506783	20.574477668826443
39	20.75623967753485	30.079446889112184	29.08654123788735	20.077772195465624
40	22.457208689927587	27.20648106441218	28.600735710243352	21.735574535416884
41	18.782266726724643	26.01073536375501	29.87220355035053	25.334794359169816
42	21.54363443170137	26.96025778732546	27.8661804280286	23.629927352944573
43	21.596329533511195	27.46497580356421	28.077033020338867	22.861661642585727
44	20.238889273875934	29.15085813612372	30.206853539378397	20.403399050621946
45	19.326253421572364	32.270119422981416	27.166490535093494	21.237136620352725
46	21.21345991707379	30.564819306330342	28.684398207500315	19.53732256909555
47	21.025201251977872	28.173327905015995	29.432740838270792	21.36873000473534
48	21.859444117205456	25.842616333460377	31.411766744049064	20.886172805285103
49	21.132612637584742	25.602528787406303	31.727504244482173	21.537354330526778
50	19.521080928126768	30.732288670986218	29.658174814917476	20.088455585969534
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	574.0
1	1157.0
2	1740.0
3	9237.5
4	16735.0
5	12399.5
6	8064.0
7	8176.5
8	8289.0
9	8329.5
10	8370.0
11	8447.0
12	8524.0
13	8055.0
14	7586.0
15	7124.0
16	6662.0
17	6560.0
18	6458.0
19	6665.0
20	6872.0
21	7690.5
22	8509.0
23	9591.0
24	10673.0
25	13410.5
26	16148.0
27	20035.5
28	23923.0
29	28370.5
30	32818.0
31	36655.0
32	40492.0
33	46386.0
34	52280.0
35	54933.0
36	57586.0
37	62683.5
38	67781.0
39	80273.5
40	92766.0
41	106719.0
42	120672.0
43	123951.0
44	127230.0
45	122878.5
46	118527.0
47	113581.0
48	108635.0
49	102958.5
50	97282.0
51	87902.0
52	78522.0
53	70459.5
54	62397.0
55	55339.0
56	48281.0
57	44654.5
58	41028.0
59	37093.0
60	33158.0
61	28325.5
62	23493.0
63	19302.5
64	15112.0
65	12515.0
66	9918.0
67	8246.5
68	6575.0
69	5710.5
70	4846.0
71	4050.5
72	3255.0
73	2604.5
74	1954.0
75	1384.5
76	815.0
77	602.5
78	390.0
79	315.5
80	241.0
81	162.5
82	84.0
83	62.5
84	41.0
85	25.0
86	9.0
87	7.5
88	6.0
89	4.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1385328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.858407696456627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.33809159094278	16.781548657674204
2	6.583207744019978	3.1412970861459817
3	3.2542492705388484	2.3292361752723725
4	2.2134059453605106	2.1123336576868827
5	1.747865910278219	2.0850648743078013
6	1.4125377892534225	2.022054147759581
7	1.2068389211268693	2.0155278502937697
8	1.0482400236615164	2.000747027860784
9	0.9246723696469937	1.9855089342617958
>10	10.539623248025702	53.07958902283298
>50	0.6879018997678639	10.215469510953385
>100	0.041966407139357474	1.352857855880734
>500	0.0	0.0
>1k	0.001049160178443714	0.4515644946225995
>5k	3.49720059481238E-4	0.4272007044471008
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5120	0.3695875633784923	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2199	0.15873497106822357	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2000	0.14437014194472356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006352286245567836	0.0
2	0.0	0.0	0.0	0.02988461938255778	0.0
3	0.0	0.0	0.0	0.04150641580910802	0.0
4	0.0	0.0	0.0	0.058542092558585404	0.0
5	0.0	0.0	0.0	0.10040943372255523	0.0
6	0.0	0.0	0.0	0.17288324497880647	0.0
7	0.0	0.0	0.0	0.21973135603986926	0.0
8	0.0	0.0	0.0	0.44696995946086415	0.0
9	0.0	0.0	0.0	0.5579905986163566	0.0
10	0.0	0.0	0.0	0.7723080743332987	0.0
11	0.0	0.0	0.0	0.942376101544183	0.0
12	0.0	0.0	0.0	1.1146818659552107	0.0
13	0.0	0.0	0.0	1.1817417968885346	0.0
14	0.0	0.0	0.0	1.2076562373676125	0.0
15	0.0	0.0	0.0	1.2461308801958815	0.0
16	0.0	0.0	0.0	1.333835741427301	0.0
17	0.0	7.218507097236178E-5	0.0	1.4442789000150145	0.0
18	0.0	7.218507097236178E-5	0.0	1.59543443863114	0.0
19	0.0	7.218507097236178E-5	0.0	1.6673307693196124	0.0
20	0.0	7.218507097236178E-5	0.0	1.737711213517665	0.0
21	0.0	7.218507097236178E-5	0.0	1.823467077832831	0.0
22	0.0	7.218507097236178E-5	0.0	1.9246705473360821	0.0
23	0.0	7.218507097236178E-5	0.0	2.033814374646293	0.0
24	0.0	7.218507097236178E-5	0.0	2.1153835048450618	0.0
25	0.0	7.218507097236178E-5	0.0	2.1795560329394914	0.0
26	0.0	7.218507097236178E-5	0.0	2.24704907429865	0.0
27	0.0	7.218507097236178E-5	0.0	2.314758670870725	0.0
28	7.218507097236178E-5	7.218507097236178E-5	0.0	2.387088111985032	0.0
29	7.218507097236178E-5	7.218507097236178E-5	0.0	2.4659863945578233	0.0
30	7.218507097236178E-5	7.218507097236178E-5	0.0	2.5703660071838583	0.0
31	7.218507097236178E-5	7.218507097236178E-5	0.0	2.6559775013570794	0.0
32	7.218507097236178E-5	7.218507097236178E-5	0.0	2.744909508795029	0.0
33	7.218507097236178E-5	7.218507097236178E-5	0.0	2.829726967187554	0.0
34	7.218507097236178E-5	7.218507097236178E-5	0.0	2.919308640264255	0.0
35	7.218507097236178E-5	7.218507097236178E-5	0.0	3.023255142464456	0.0
36	7.218507097236178E-5	7.218507097236178E-5	0.0	3.110599078341014	0.0
37	7.218507097236178E-5	7.218507097236178E-5	0.0	3.2071105182310617	0.0
38	7.218507097236178E-5	7.218507097236178E-5	0.0	3.3034775879791645	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCAAC	20	7.857042E-4	44.000004	13
CGCCTAT	20	7.857042E-4	44.000004	30
CGTAAAT	40	8.314601E-9	44.000004	16
CGTTATT	1900	0.0	42.726315	1
CGTTTTA	3365	0.0	42.692425	1
AGGCGAT	1195	0.0	42.343098	7
AGTACGG	105	0.0	41.90476	2
TTACGGG	385	0.0	41.714287	3
TTGCGAG	90	0.0	41.555557	2
CACGACG	185	0.0	40.43243	26
GCGCAAA	60	3.6379788E-12	40.333332	1
CGGTCTA	180	0.0	40.333332	31
ACGCATT	50	1.3478711E-9	39.600002	29
CGAAGGA	500	0.0	39.6	4
CGACGGT	195	0.0	39.48718	28
ACGGTCT	185	0.0	39.24324	30
TACGGGA	390	0.0	38.923077	4
GCGAATA	40	4.1253588E-7	38.500004	1
GCTAACG	40	4.1253588E-7	38.500004	1
TAGGCGA	360	0.0	38.5	6
>>END_MODULE