FastQCFastQC Report
Thu 26 May 2016
SRR1545767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545767_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences531115
Sequences flagged as poor quality0
Sequence length51
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14100.26547922766255894No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11570.2178435931954473No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG253.8879614E-545.02
GCGGTCA207.029857E-445.09
GCGCAAA253.8879614E-545.01
GCGGAAA253.8879614E-545.01
TACACCC207.029857E-445.014
CGCGAAG302.1632877E-644.9999962
AGGCGAT4350.043.965527
CGTTTTA10450.042.846891
CGTTTTT12000.042.7500041
GCTACGG603.6379788E-1241.2499962
CGAAGGA2400.041.2499964
CGAATAT556.002665E-1140.90909214
ACGGGAT1550.040.6451645
GGCGATT2500.040.5000048
CGCATGG501.0786607E-940.52
ACACGAC451.9250365E-840.00000426
CGACGGT451.9250365E-840.00000428
CACGACG451.9250365E-840.00000426
CACGACC451.9250365E-840.00000427
ACCCGCT1300.039.8076934