FastQCFastQC Report
Thu 26 May 2016
SRR1545766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545766_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences584908
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19700.3368051043924857No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19610.33526640086988035No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8350.14275749348615507No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8090.13831234997640654No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGGT650.044.00000428
CAGGTAT207.8533095E-444.06
AACGTAG207.8533095E-444.02
CGGGTCC254.4401502E-544.024
CTATGCG254.4401502E-544.025
CGGAACC207.8533095E-444.029
AGTCCCG207.8533095E-444.022
CAAACCG207.8533095E-444.012
GTACAGC254.4401502E-544.024
CGAAATC207.8533095E-444.026
ACGTTAG302.5255758E-644.02
TAGACGG502.7284841E-1144.02
AACCCCG207.8533095E-444.011
CGTTGTG207.8533095E-444.031
CTATACG351.4444595E-744.01
GCTATAG502.7284841E-1144.02
ATAGCCG207.8533095E-444.019
GCAAACG254.4401502E-544.01
TTCGTCC207.8533095E-444.030
TTACTAA207.8533095E-444.01
Produced by FastQC (version 0.11.5)