##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545766_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	584908
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.57715059462343	31.0	31.0	33.0	30.0	34.0
2	31.75961347767512	31.0	31.0	34.0	30.0	34.0
3	31.779072948224336	31.0	31.0	34.0	30.0	34.0
4	35.669965191106975	37.0	35.0	37.0	35.0	37.0
5	26.995132567856825	35.0	22.0	37.0	0.0	37.0
6	31.289436971284374	35.0	25.0	37.0	17.0	37.0
7	15.095165393531975	0.0	0.0	32.0	0.0	37.0
8	25.179140993113446	17.0	17.0	35.0	17.0	37.0
9	33.38897057314997	32.0	32.0	37.0	28.0	39.0
10	36.21283689058792	37.0	35.0	39.0	33.0	39.0
11	37.125703529443946	39.0	37.0	39.0	34.0	39.0
12	36.818292791344966	39.0	35.0	39.0	33.0	39.0
13	36.06825175925103	38.0	35.0	39.0	32.0	39.0
14	37.33923967529936	39.0	35.0	41.0	33.0	41.0
15	37.72565771027239	40.0	35.0	41.0	33.0	41.0
16	37.872032866707244	40.0	35.0	41.0	33.0	41.0
17	37.92094654202028	40.0	35.0	41.0	34.0	41.0
18	37.912010093895105	40.0	36.0	41.0	34.0	41.0
19	37.823428641769304	40.0	36.0	41.0	34.0	41.0
20	37.80930847244353	39.0	35.0	41.0	34.0	41.0
21	37.62381092411114	39.0	35.0	41.0	33.0	41.0
22	37.54098935217162	39.0	35.0	41.0	33.0	41.0
23	37.46119731650106	39.0	35.0	41.0	33.0	41.0
24	37.31621725126003	39.0	35.0	41.0	33.0	41.0
25	37.16135529006271	38.0	35.0	41.0	33.0	41.0
26	36.92426843195853	38.0	35.0	40.0	33.0	41.0
27	36.68294501015544	38.0	35.0	40.0	32.0	41.0
28	36.76782502547409	38.0	35.0	40.0	32.0	41.0
29	36.78331122159382	38.0	35.0	40.0	33.0	41.0
30	36.58723423170824	38.0	35.0	40.0	32.0	41.0
31	36.34085702366868	38.0	35.0	40.0	31.0	41.0
32	36.00628816839571	38.0	35.0	40.0	31.0	41.0
33	35.78111600456824	38.0	35.0	40.0	30.0	41.0
34	35.58080074131316	38.0	35.0	40.0	30.0	41.0
35	35.41973609524917	38.0	34.0	40.0	29.0	41.0
36	35.21057841575085	38.0	34.0	40.0	28.0	41.0
37	34.991781613518704	37.0	34.0	40.0	27.0	41.0
38	34.84713493404091	37.0	34.0	40.0	27.0	41.0
39	34.88261743727219	37.0	34.0	40.0	27.0	41.0
40	34.68346987902371	37.0	34.0	40.0	26.0	41.0
41	34.54399837239361	37.0	34.0	40.0	25.0	40.0
42	34.534877279845716	37.0	34.0	40.0	25.0	41.0
43	34.4179289734454	37.0	33.0	40.0	24.0	41.0
44	34.194938349278864	36.0	33.0	40.0	24.0	40.0
45	33.922403181355016	36.0	33.0	39.0	23.0	40.0
46	33.952667769974084	36.0	33.0	39.0	23.0	40.0
47	33.83493130543607	36.0	33.0	39.0	23.0	40.0
48	33.85924111142265	36.0	33.0	39.0	23.0	40.0
49	33.8011909565265	36.0	33.0	39.0	24.0	40.0
50	33.78585008240612	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	3.0
15	8.0
16	32.0
17	82.0
18	227.0
19	420.0
20	793.0
21	1459.0
22	2239.0
23	3516.0
24	5251.0
25	7824.0
26	10199.0
27	10977.0
28	11105.0
29	12479.0
30	14884.0
31	20295.0
32	28839.0
33	59480.0
34	53562.0
35	61293.0
36	68357.0
37	133933.0
38	64032.0
39	13616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.23090810862563	23.141930012925112	29.930860921717606	16.69630095673166
2	31.952033482188654	23.570031526325508	31.66036368112592	12.81757131035992
3	28.53252135378555	23.60422493793896	35.2710169804482	12.592236727827283
4	24.483508517578834	26.95757281487003	34.33206589754286	14.226852770008275
5	17.16509262995206	47.617745012890914	24.840487734823256	10.376674622333768
6	19.831494867568917	39.40636818097889	29.913251314736677	10.848885636715517
7	39.46398407954755	55.46427814288742	4.175528459176485	0.896209318388533
8	88.71395159580652	2.349600279018239	7.769768920924316	1.166679204250925
9	86.13234901899102	3.22973869394845	8.735048930772018	1.9028633562885102
10	60.2793943662935	16.963180534374636	13.521100754306662	9.2363243450252
11	54.89666751010416	16.73972658948074	17.75253544147114	10.61107045894397
12	48.396499962387246	19.34235811443851	21.641010210152707	10.620131713021536
13	20.42834086728169	45.73830414355762	22.767170221641695	11.066184767518994
14	13.00170283189835	48.45907390563986	27.775992121837966	10.763231140623825
15	11.114226510835893	21.268985891798366	55.478810342823145	12.137977254542594
16	12.80509071512101	14.61580282711127	54.291957025720286	18.287149432047435
17	13.623339055030875	14.527925759264704	30.526852086140043	41.321883099564374
18	20.860374623017634	20.27669308677604	38.398175439556304	20.46475685065002
19	30.438291150061207	21.259069802430467	26.99997264527071	21.302666402237616
20	33.69247813331327	21.300956731656946	24.50487939983724	20.50168573519254
21	21.377208039554937	27.73530196201796	29.845719326800115	21.041770671626992
22	22.456181143017364	22.53277438503149	26.916369753875824	28.094674718075318
23	17.946070151203266	31.16267857509215	24.660801356794572	26.230449916910008
24	19.115313861325202	21.886347938479215	42.6639745053923	16.334363694803287
25	15.460551061021564	22.365739569299787	41.006277910372226	21.16743145930642
26	14.6433285234601	36.13884576719757	28.982848584734693	20.23497712460763
27	15.726233869258072	35.23579776648636	31.232091200667455	17.805877163588118
28	12.955883660336326	28.038768490087328	43.97358217018745	15.031765679388895
29	13.15420544769434	22.665102887975543	42.876315591511826	21.30437607281829
30	16.354879741771356	30.099605408029984	34.79572855902125	18.74978629117742
31	31.790469612315096	24.423670047255293	24.61122090995507	19.174639430474535
32	31.965197945659828	25.978615440376952	26.420736252538862	15.635450361424361
33	29.31144727033995	26.414239504332304	25.552565531673356	18.721747693654386
34	18.833731116688437	27.57852516977029	29.215021849590023	24.372721863951256
35	18.780560361629522	24.627633747529526	33.81608731629589	22.775718574545056
36	33.42576952272836	21.679819732333975	26.628803162206705	18.265607582730958
37	19.937152509454478	32.32935777934308	28.702804543620537	19.03068516758191
38	19.737121051515793	34.97541493704993	21.937638055899388	23.349825955534886
39	19.97066205283566	31.923311016433352	27.248387780642425	20.857639150088563
40	23.635511909565267	25.05043528212984	24.994700021199915	26.319352787104982
41	16.591327183078363	22.58064516129032	27.527576986466247	33.300450669165066
42	22.24674649688498	24.532405096186068	23.364871056644805	29.855977350284146
43	21.949092848789896	26.038966811874687	25.06274491031068	26.949195429024737
44	19.02384648525922	30.32442708938842	28.96233253776662	21.68939388758574
45	15.85326239340204	39.66709294453145	22.011324857926375	22.468319804140137
46	22.307952703673056	33.155812538040166	26.174714655980086	18.361520102306688
47	21.792316056542223	27.24360070301654	26.07435699289461	24.889726247546623
48	23.676714970559473	22.20930471116825	31.557954413343637	22.55602590492864
49	20.98774508127774	21.21530223556525	34.138531187810734	23.65842149534628
50	18.98366922661342	33.77009717767581	26.803018594377235	20.44321500133354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	426.0
1	562.0
2	698.0
3	3559.0
4	6420.0
5	4850.5
6	3281.0
7	3518.5
8	3756.0
9	4206.5
10	4657.0
11	4627.5
12	4598.0
13	4293.0
14	3988.0
15	3714.0
16	3440.0
17	3069.5
18	2699.0
19	2722.5
20	2746.0
21	2548.5
22	2351.0
23	2391.5
24	2432.0
25	2450.0
26	2468.0
27	2914.5
28	3361.0
29	4310.5
30	5260.0
31	6396.5
32	7533.0
33	8574.0
34	9615.0
35	10213.0
36	10811.0
37	14730.0
38	18649.0
39	30077.0
40	41505.0
41	53240.0
42	64975.0
43	67625.5
44	70276.0
45	66299.5
46	62323.0
47	57093.5
48	51864.0
49	48331.5
50	44799.0
51	40479.5
52	36160.0
53	32513.0
54	28866.0
55	25678.0
56	22490.0
57	20556.0
58	18622.0
59	16641.5
60	14661.0
61	12984.5
62	11308.0
63	9076.0
64	6844.0
65	5493.0
66	4142.0
67	3507.5
68	2873.0
69	2468.5
70	2064.0
71	1523.0
72	982.0
73	771.5
74	561.0
75	393.5
76	226.0
77	162.5
78	99.0
79	73.5
80	48.0
81	32.5
82	17.0
83	14.5
84	12.0
85	6.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	584908.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.263628231532955
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.5002704504363	16.866193384445445
2	5.920212624602202	2.7545125110074715
3	2.7557718654809493	1.9232775650840048
4	1.9411448110685756	1.8063228491307446
5	1.4739669446563062	1.7144909513026405
6	1.2207648095583972	1.7039651212622804
7	1.083391153478298	1.764252631669555
8	0.9425115074998748	1.7540989851535016
9	0.8368576007304442	1.7521509695513127
>10	10.423649788150895	53.02865700550673
>50	0.8457211126598342	12.384415070549815
>100	0.05276467398844308	1.5838178836752177
>500	0.0014863288447448755	0.28422624175985783
>1k	0.0014863288447448755	0.6796188299014606
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1970	0.3368051043924857	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1961	0.33526640086988035	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	835	0.14275749348615507	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	809	0.13831234997640654	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00854835290336258	0.0
2	0.0	0.0	0.0	0.034535345729584825	0.0
3	0.0	0.0	0.0	0.048212710374964954	0.0
4	0.0	0.0	0.0	0.07317390085278369	0.0
5	0.0	0.0	0.0	0.11659953360186559	0.0
6	0.0	0.0	0.0	0.19165407209338906	0.0
7	0.0	0.0	0.0	0.24243128833936278	0.0
8	0.0	0.0	0.0	0.5170043835953688	0.0
9	0.0	0.0	0.0	0.6262523337003426	0.0
10	0.0	0.0	0.0	0.8237192857680182	0.0
11	0.0	0.0	0.0	0.9777606050866119	0.0
12	0.0	0.0	0.0	1.1340244961600798	0.0
13	0.0	0.0	0.0	1.1921532959029455	0.0
14	0.0	0.0	0.0	1.2145499805097555	0.0
15	0.0	0.0	0.0	1.2501111285877438	0.0
16	0.0	0.0	0.0	1.3362785258536385	0.0
17	0.0	0.0	0.0	1.4364652218810479	0.0
18	0.0	0.0	0.0	1.6041839058450218	0.0
19	0.0	0.0	0.0	1.6657320467492323	0.0
20	0.0	0.0	0.0	1.7245447147243669	0.0
21	0.0	0.0	0.0	1.8158411237322793	0.0
22	0.0	0.0	0.0	1.9006407845336362	0.0
23	0.0	0.0	0.0	2.0069822946514666	0.0
24	0.0	0.0	0.0	2.077933623749376	0.0
25	0.0	0.0	0.0	2.1278560047050132	0.0
26	0.0	0.0	0.0	2.191968651480233	0.0
27	0.0	0.0	0.0	2.251465187687636	0.0
28	0.0	0.0	0.0	2.310448822720838	0.0
29	0.0	0.0	0.0	2.3745614694960575	0.0
30	0.0	0.0	0.0	2.461070800878087	0.0
31	0.0	0.0	0.0	2.547409165202049	0.0
32	0.0	0.0	0.0	2.6204120989967654	0.0
33	0.0	0.0	0.0	2.6946118021979526	0.0
34	0.0	0.0	0.0	2.7718889124443504	0.0
35	0.0	0.0	0.0	2.8881465119300813	0.0
36	0.0	0.0	0.0	2.967988128047488	0.0
37	0.0	0.0	0.0	3.0500523159197686	0.0
38	0.0	0.0	0.0	3.1336552073146544	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGGT	65	0.0	44.000004	28
CAGGTAT	20	7.8533095E-4	44.0	6
AACGTAG	20	7.8533095E-4	44.0	2
CGGGTCC	25	4.4401502E-5	44.0	24
CTATGCG	25	4.4401502E-5	44.0	25
CGGAACC	20	7.8533095E-4	44.0	29
AGTCCCG	20	7.8533095E-4	44.0	22
CAAACCG	20	7.8533095E-4	44.0	12
GTACAGC	25	4.4401502E-5	44.0	24
CGAAATC	20	7.8533095E-4	44.0	26
ACGTTAG	30	2.5255758E-6	44.0	2
TAGACGG	50	2.7284841E-11	44.0	2
AACCCCG	20	7.8533095E-4	44.0	11
CGTTGTG	20	7.8533095E-4	44.0	31
CTATACG	35	1.4444595E-7	44.0	1
GCTATAG	50	2.7284841E-11	44.0	2
ATAGCCG	20	7.8533095E-4	44.0	19
GCAAACG	25	4.4401502E-5	44.0	1
TTCGTCC	20	7.8533095E-4	44.0	30
TTACTAA	20	7.8533095E-4	44.0	1
>>END_MODULE