FastQCFastQC Report
Thu 26 May 2016
SRR1545765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545765_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences226182
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7200.3183277183860785No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5450.24095639794501772No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4970.21973455005261253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG302.1577453E-645.0000043
AGGACTA302.1577453E-645.0000047
AGGAACG302.1577453E-645.0000047
ACGGGAT302.1577453E-645.0000045
CTTGCTA302.1577453E-645.0000041
GCGATAT302.1577453E-645.0000049
TGGGCGA750.045.0000046
AAGGCGC302.1577453E-645.0000046
TCACGAC551.8189894E-1245.025
CTCACGA551.8189894E-1245.024
GTACCCA207.02126E-445.028
AGATCGA207.02126E-445.01
GATGTAG253.8808397E-545.01
CATATAG207.02126E-445.02
CACGGGC207.02126E-445.04
CGACGGG253.8808397E-545.03
CCTAGGA207.02126E-445.04
CGGTTTA207.02126E-445.01
TAGGTGA207.02126E-445.06
TATGGGC207.02126E-445.04
Produced by FastQC (version 0.11.5)