##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545765_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	226182
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.037704149755506	31.0	31.0	33.0	30.0	33.0
2	31.1898029020877	31.0	31.0	33.0	30.0	34.0
3	31.108713337047156	31.0	31.0	34.0	28.0	34.0
4	35.04923910832869	35.0	35.0	37.0	32.0	37.0
5	32.769981696156194	35.0	35.0	37.0	28.0	37.0
6	33.97702292843816	35.0	35.0	37.0	28.0	37.0
7	15.582305400076045	0.0	0.0	35.0	0.0	37.0
8	25.072322289129993	17.0	17.0	35.0	17.0	37.0
9	32.90479790611101	32.0	32.0	37.0	28.0	39.0
10	35.65945123838325	37.0	35.0	38.0	32.0	39.0
11	36.55975718669036	37.0	35.0	39.0	32.0	39.0
12	36.141540883005725	37.0	35.0	39.0	32.0	39.0
13	35.479016897896386	37.0	35.0	39.0	30.0	39.0
14	36.45963427682132	38.0	35.0	40.0	31.0	41.0
15	36.986877824053195	39.0	35.0	40.0	31.0	41.0
16	37.16127720154566	39.0	35.0	41.0	32.0	41.0
17	37.15818234872802	39.0	35.0	41.0	32.0	41.0
18	37.08146536859697	38.0	35.0	40.0	32.0	41.0
19	36.960770529927224	38.0	35.0	40.0	32.0	41.0
20	37.01848511375795	39.0	35.0	40.0	32.0	41.0
21	36.80938359374309	38.0	35.0	40.0	32.0	41.0
22	36.86779230884863	38.0	35.0	40.0	32.0	41.0
23	36.89996109327886	38.0	35.0	40.0	32.0	41.0
24	36.729514284956366	38.0	35.0	40.0	32.0	41.0
25	36.51325481249613	38.0	35.0	40.0	31.0	41.0
26	36.36270348657276	38.0	35.0	40.0	31.0	41.0
27	36.20849581310626	38.0	34.0	40.0	31.0	41.0
28	36.19440096913105	38.0	35.0	40.0	31.0	41.0
29	36.269119558585565	38.0	35.0	40.0	31.0	41.0
30	36.039782122361636	38.0	35.0	40.0	30.0	41.0
31	35.868212324588164	38.0	34.0	40.0	30.0	41.0
32	35.66572494716644	38.0	34.0	40.0	30.0	41.0
33	35.44547311457145	38.0	34.0	40.0	29.0	41.0
34	35.29644268774704	38.0	34.0	40.0	27.0	41.0
35	35.07631464926475	38.0	34.0	40.0	27.0	41.0
36	34.867650829862676	37.0	34.0	40.0	25.0	41.0
37	34.706789222838246	37.0	33.0	40.0	24.0	41.0
38	34.67262646894979	37.0	33.0	40.0	24.0	41.0
39	34.62821533101661	37.0	33.0	40.0	24.0	41.0
40	34.46091643013149	37.0	33.0	40.0	23.0	41.0
41	34.37407043885013	37.0	33.0	40.0	23.0	41.0
42	34.326896039472636	37.0	33.0	40.0	23.0	41.0
43	34.231508254414585	37.0	33.0	40.0	23.0	41.0
44	33.91270746566924	36.0	33.0	40.0	23.0	41.0
45	33.868163691186744	36.0	33.0	40.0	23.0	41.0
46	33.90061101237057	36.0	33.0	40.0	23.0	41.0
47	33.889650812177806	36.0	33.0	40.0	23.0	41.0
48	33.93229788400492	36.0	33.0	40.0	23.0	41.0
49	33.89194984569948	36.0	33.0	40.0	23.0	41.0
50	33.7300536735903	36.0	33.0	40.0	23.0	41.0
51	33.506481506043805	35.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	6.0
15	12.0
16	29.0
17	66.0
18	147.0
19	311.0
20	504.0
21	846.0
22	1231.0
23	1885.0
24	2717.0
25	3443.0
26	4304.0
27	4618.0
28	4971.0
29	5661.0
30	6776.0
31	8983.0
32	11894.0
33	19117.0
34	23958.0
35	22750.0
36	23915.0
37	36694.0
38	35327.0
39	6017.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.600940835256562	23.93028622967345	30.29286150091519	16.175911434154795
2	31.37738635258332	23.353317240098683	32.24571362884757	13.023582778470436
3	28.985065124545724	23.388686986586023	34.92320343793936	12.7030444509289
4	24.98828377147607	27.589286503788983	32.93498156351964	14.487448161215305
5	20.908825635992255	35.989159172701626	30.197805307230464	12.904209884075657
6	20.046245943532202	40.061985480719066	29.42630271197531	10.46546586377342
7	38.68787082968583	56.01153053735487	4.620615256740147	0.679983376219151
8	89.35237994181676	2.570496325967584	7.03902167281216	1.038102059403489
9	86.66825830525859	3.3203349514992353	7.999310289943497	2.0120964532986707
10	60.551237499005225	17.24805687455235	12.81003793405311	9.390667692389314
11	55.32270472451388	17.057502365351795	17.095082720994597	10.524710189139718
12	48.82484017295806	19.64391507723868	20.90794139233007	10.623303357473185
13	20.57148667886923	46.37725371603399	21.953117401031026	11.098142204065752
14	12.899346543933646	49.16394761740545	27.269632419909627	10.667073418751272
15	11.096373716741386	21.67944398758522	55.218363972376224	12.005818323297168
16	12.888735619987443	14.821692265520687	54.043204145334286	18.246367969157582
17	13.590825087761182	14.726636071835955	30.130602788904508	41.55193605149835
18	20.744356314826113	20.24299015836804	38.60519404727167	20.407459479534182
19	31.192137305355867	21.169677516336403	26.628555764826555	21.00962941348118
20	34.50274557657108	21.312040745947954	23.992183286026297	20.19303039145467
21	21.48048916359392	28.597324278678233	29.21585272037563	20.70633383735222
22	22.30769910956663	22.580930401181348	26.249215233749812	28.86215525550221
23	17.772413366227198	31.66697615194843	24.23446604946459	26.326144432359783
24	18.665057343201493	21.957096497510854	43.18203924273373	16.195806916553927
25	15.496370179766735	22.298856672944797	41.39277219230531	20.812000954983155
26	14.582946476731129	36.94679505884642	28.40765401313986	20.062604451282596
27	15.466748017083587	36.639520386237635	30.47413145166282	17.41960014501596
28	12.831701903776604	28.57433394346146	44.063630173930726	14.530333978831205
29	13.068679205241796	22.96469215056901	42.980431687755875	20.986196956433314
30	16.293073719394116	31.18727396521385	34.16894359409679	18.350708721295238
31	32.5392825246925	24.457737574165936	24.315816466385478	18.687163434756084
32	33.223687119222575	25.594874923733986	26.083861668921486	15.097576288121955
33	30.478110548142645	26.166980573166743	24.776507414383108	18.578401464307504
34	19.043955752447143	27.515452157996656	28.386874287078545	25.05371780247765
35	18.889655233396116	24.325543146669496	34.121194436338875	22.66360718359551
36	34.242335818058024	22.019435675694794	26.01179581045353	17.726432695793655
37	19.802636814600632	33.380640369260156	28.297565677198012	18.519157138941207
38	19.66248419414454	35.73714972897932	21.636116048138224	22.964250028737922
39	20.166945203420255	32.64981298246545	26.8734912592514	20.309750554862898
40	24.409546294576934	24.808340186221713	24.776507414383108	26.005606104818245
41	16.558788939880273	22.46597872509749	27.32666613612047	33.64856619890177
42	21.91597916721932	24.01517362124307	23.386918499261654	30.68192871227595
43	21.54769168191987	25.4060889018578	25.076708137694425	27.969511278527907
44	18.546126570637806	30.13016066707342	29.805643243052053	21.51806951923672
45	15.758548425604161	40.47360090546551	21.877072446083243	21.89077822284709
46	22.08840668134511	33.956282993341645	25.677551706148144	18.2777586191651
47	21.29126101988664	27.482293020664773	25.62317072092386	25.603275238524724
48	23.289209574590373	22.588888594141	31.986188113996693	22.135713717271933
49	21.007860926156813	21.63257907348949	34.38071995118975	22.97884004916395
50	19.226109946856955	34.92585616892591	26.130284461186125	19.717749423031012
51	18.247252212819763	36.837590966566744	22.564129771599863	22.351027049013627
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	210.5
2	247.0
3	1179.5
4	2112.0
5	1658.5
6	1205.0
7	1256.0
8	1307.0
9	1440.0
10	1573.0
11	1527.0
12	1481.0
13	1376.5
14	1272.0
15	1244.5
16	1217.0
17	1102.0
18	987.0
19	1001.5
20	1016.0
21	984.0
22	952.0
23	930.5
24	909.0
25	987.0
26	1088.5
27	1112.0
28	1495.5
29	1879.0
30	2306.5
31	2734.0
32	3175.5
33	3617.0
34	3823.0
35	4029.0
36	5072.0
37	6115.0
38	9735.5
39	13356.0
40	17872.5
41	22389.0
42	24278.5
43	26168.0
44	25411.0
45	24654.0
46	23038.0
47	21422.0
48	19531.5
49	17641.0
50	16273.0
51	14905.0
52	13713.0
53	12521.0
54	11222.0
55	9923.0
56	9047.0
57	8171.0
58	7185.5
59	6200.0
60	5545.0
61	4890.0
62	4138.0
63	3386.0
64	2745.0
65	2104.0
66	1654.0
67	1204.0
68	1077.5
69	951.0
70	796.0
71	641.0
72	493.0
73	345.0
74	260.5
75	120.0
76	64.0
77	49.5
78	35.0
79	25.5
80	16.0
81	12.0
82	8.0
83	7.5
84	7.0
85	4.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	226182.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.142619660273585
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.46262723775182	21.00962941348118
2	7.585286560002272	4.724513887046715
3	4.301594287255639	4.01888744462424
4	3.0366700265477933	3.7827943868212324
5	2.31973764533852	3.612135360019807
6	1.83563082951206	3.429981165609995
7	1.5744119024971963	3.4321917747654545
8	1.4225074177657262	3.544048598031674
9	1.2947372904214995	3.6289359896012945
>10	9.10433140731697	46.87729350699879
>50	0.055367055182498334	1.0473866178564166
>100	0.004259004244807563	0.33291773881210707
>500	0.002839336163205043	0.5592841163310962
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	720	0.3183277183860785	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	545	0.24095639794501772	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.21973455005261253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006631827466376635	0.0
2	0.0	0.0	0.0	0.031832771838607846	0.0
3	0.0	0.0	0.0	0.04111733029153514	0.0
4	0.0	0.0	0.0	0.06985524931250055	0.0
5	0.0	0.0	0.0	0.12600472186115605	0.0
6	0.0	0.0	0.0	0.19276511835601418	0.0
7	0.0	0.0	0.0	0.23122971766099867	0.0
8	0.0	0.0	0.0	0.4558276078556207	0.0
9	0.0	0.0	0.0	0.542041364918517	0.0
10	0.0	0.0	0.0	0.7241955593283285	0.0
11	0.0	0.0	0.0	0.8586005959802283	0.0
12	0.0	0.0	0.0	0.9987532164363212	0.0
13	0.0	0.0	0.0	1.0451760087009576	0.0
14	0.0	0.0	0.0	1.0606502727891698	0.0
15	0.0	0.0	0.0	1.0889460699790434	0.0
16	0.0	0.0	0.0	1.1711807305621138	0.0
17	0.0	0.0	0.0	1.2542996348073676	0.0
18	0.0	0.0	0.0	1.4019683263920206	0.0
19	0.0	0.0	0.0	1.4559071897852172	0.0
20	0.0	0.0	0.0	1.5142672714893315	0.0
21	0.0	0.0	0.0	1.5947334447480348	0.0
22	0.0	0.0	0.0	1.671220521526912	0.0
23	0.0	0.0	0.0	1.7640661060561849	0.0
24	0.0	0.0	0.0	1.8241946750846663	0.0
25	0.0	0.0	0.0	1.867964736362752	0.0
26	0.0	0.0	0.0	1.9241142089114076	0.0
27	0.0	0.0	0.0	1.9692106356827688	0.0
28	0.0	0.0	0.0	2.017401915271772	0.0
29	0.0	0.0	0.0	2.0748777533137033	0.0
30	0.0	0.0	0.0	2.1575545357278654	0.0
31	0.0	0.0	0.0	2.237136465324385	0.0
32	0.0	0.0	0.0	2.297265034352866	0.0
33	0.0	0.0	0.0	2.362256943523357	0.0
34	0.0	0.0	0.0	2.435649167484592	0.0
35	0.0	0.0	0.0	2.552369330892821	0.0
36	0.0	0.0	0.0	2.6297406513338815	0.0
37	0.0	0.0	0.0	2.7049013626194833	0.0
38	0.0	0.0	0.0	2.774756611931984	0.0
39	0.0	0.0	0.0	2.8698128056167156	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGTAGG	30	2.1577453E-6	45.000004	3
AGGACTA	30	2.1577453E-6	45.000004	7
AGGAACG	30	2.1577453E-6	45.000004	7
ACGGGAT	30	2.1577453E-6	45.000004	5
CTTGCTA	30	2.1577453E-6	45.000004	1
GCGATAT	30	2.1577453E-6	45.000004	9
TGGGCGA	75	0.0	45.000004	6
AAGGCGC	30	2.1577453E-6	45.000004	6
TCACGAC	55	1.8189894E-12	45.0	25
CTCACGA	55	1.8189894E-12	45.0	24
GTACCCA	20	7.02126E-4	45.0	28
AGATCGA	20	7.02126E-4	45.0	1
GATGTAG	25	3.8808397E-5	45.0	1
CATATAG	20	7.02126E-4	45.0	2
CACGGGC	20	7.02126E-4	45.0	4
CGACGGG	25	3.8808397E-5	45.0	3
CCTAGGA	20	7.02126E-4	45.0	4
CGGTTTA	20	7.02126E-4	45.0	1
TAGGTGA	20	7.02126E-4	45.0	6
TATGGGC	20	7.02126E-4	45.0	4
>>END_MODULE