FastQCFastQC Report
Thu 26 May 2016
SRR1545764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545764_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences739629
Sequences flagged as poor quality0
Sequence length50
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.3619382149699376No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17820.24093160219515458No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11720.15845782142128012No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.1507512550210984No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC207.854659E-444.00000417
CTCACCG207.854659E-444.00000442
GCGACTA408.305506E-944.0000041
CGTATTA207.854659E-444.00000435
TCACCCG207.854659E-444.00000432
ACGCATT207.854659E-444.00000414
CGCATCG408.305506E-944.00000421
TACGGGT408.305506E-944.0000044
TACGGGA1750.044.0000044
TAACCGT207.854659E-444.00000440
GCCGACC207.854659E-444.00000414
TCGTGCG207.854659E-444.00000410
CTCGCGA207.854659E-444.0000041
ACCACGG302.5264872E-644.0000042
CGTGTAA207.854659E-444.0000042
ACCGTTA207.854659E-444.00000442
GCGTGTA207.854659E-444.0000041
AGGATCG600.044.0000047
CGCAAAA207.854659E-444.00000423
TTCGCGA207.854659E-444.00000415
Produced by FastQC (version 0.11.5)