##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545764_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	739629
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54387267129872	31.0	31.0	33.0	30.0	34.0
2	31.721803769186984	31.0	31.0	34.0	30.0	34.0
3	31.757276959124102	31.0	31.0	34.0	30.0	34.0
4	35.651119682976194	37.0	35.0	37.0	33.0	37.0
5	26.977902434869375	35.0	22.0	37.0	0.0	37.0
6	31.276644101299436	35.0	25.0	37.0	17.0	37.0
7	15.176149123411873	0.0	0.0	32.0	0.0	37.0
8	25.220730934022328	17.0	17.0	35.0	17.0	37.0
9	33.35309864810601	32.0	32.0	37.0	28.0	39.0
10	36.12978533832503	37.0	35.0	39.0	32.0	39.0
11	37.045944656037015	39.0	37.0	39.0	34.0	39.0
12	36.87008351484325	39.0	35.0	39.0	33.0	39.0
13	36.27181735708037	39.0	35.0	39.0	32.0	39.0
14	37.5908692060479	40.0	35.0	41.0	33.0	41.0
15	37.938051374405276	40.0	36.0	41.0	33.0	41.0
16	38.04238070708423	40.0	36.0	41.0	33.0	41.0
17	38.0708950027649	40.0	36.0	41.0	34.0	41.0
18	38.05516143904579	40.0	36.0	41.0	34.0	41.0
19	37.9779362355992	40.0	36.0	41.0	34.0	41.0
20	37.97410864095377	40.0	36.0	41.0	34.0	41.0
21	37.802463126783834	40.0	35.0	41.0	34.0	41.0
22	37.73901239675567	39.0	35.0	41.0	33.0	41.0
23	37.63211150455161	39.0	35.0	41.0	33.0	41.0
24	37.48210116152828	39.0	35.0	41.0	33.0	41.0
25	37.307613681994624	39.0	35.0	41.0	33.0	41.0
26	37.07503086006633	39.0	35.0	40.0	33.0	41.0
27	36.830081027109536	38.0	35.0	40.0	32.0	41.0
28	36.90387478046426	38.0	35.0	40.0	32.0	41.0
29	36.89756621224966	38.0	35.0	40.0	32.0	41.0
30	36.688835889344524	38.0	35.0	40.0	32.0	41.0
31	36.466268899678084	38.0	35.0	40.0	31.0	41.0
32	36.17084916897526	38.0	35.0	40.0	31.0	41.0
33	35.94638393032182	38.0	35.0	40.0	30.0	41.0
34	35.72797848651148	38.0	35.0	40.0	30.0	41.0
35	35.56987489673877	38.0	35.0	40.0	29.0	41.0
36	35.349118274161775	38.0	35.0	40.0	28.0	41.0
37	35.17715368110228	38.0	34.0	40.0	27.0	41.0
38	35.00465503651155	38.0	34.0	40.0	27.0	41.0
39	34.98996523932945	38.0	34.0	40.0	27.0	41.0
40	34.77755334093174	37.0	34.0	40.0	26.0	41.0
41	34.607108428685194	37.0	34.0	40.0	25.0	40.0
42	34.63363659348133	37.0	34.0	40.0	25.0	41.0
43	34.52053123931052	37.0	34.0	40.0	24.0	41.0
44	34.307533912272234	37.0	34.0	40.0	24.0	40.0
45	34.07573661930508	36.0	33.0	40.0	23.0	40.0
46	34.05042392875347	36.0	33.0	39.0	23.0	40.0
47	33.90507267832927	36.0	33.0	39.0	23.0	40.0
48	33.9038274594425	36.0	33.0	39.0	23.0	40.0
49	33.813882365348036	36.0	33.0	39.0	24.0	40.0
50	33.84167062135206	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	7.0
15	12.0
16	32.0
17	113.0
18	259.0
19	565.0
20	1087.0
21	1821.0
22	2890.0
23	4541.0
24	6997.0
25	10014.0
26	12399.0
27	13227.0
28	13602.0
29	14923.0
30	18263.0
31	24487.0
32	35104.0
33	66759.0
34	64824.0
35	76413.0
36	92571.0
37	177695.0
38	82124.0
39	18899.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.18059324336931	23.00017981988267	28.945457790324607	15.873769146423408
2	32.25833492196764	23.436885249226304	31.59665183490642	12.708127993899646
3	28.25227242306616	23.325748449560525	35.930446210194575	12.491532917178748
4	25.006320736477345	26.824123986485116	33.6972995920928	14.472255684944749
5	16.930379960764114	48.01258468772858	24.6253189098859	10.431716441621408
6	19.630517462133042	40.23963365416986	29.27656973969382	10.853279144003277
7	39.691926628079756	55.21335696680363	4.204945993193885	0.8897704119227343
8	88.04008496151448	2.4834072217287315	8.046466539305516	1.4300412774512627
9	84.74681225317016	3.7441744442145994	9.3841642228739	2.1248490797413297
10	53.96083712239515	22.74356467904855	14.445350304003766	8.850247894552538
11	48.73159381257361	18.583100446304837	21.36246685838441	11.322838882737155
12	44.53246154490968	20.20472426040623	24.000411016874676	11.262403177809416
13	20.157673644489332	43.31631128579328	24.040971892665105	12.485043177052278
14	13.716200960211133	44.65049369345983	27.836793852052853	13.796511494276183
15	11.547140525858236	22.690294728843785	52.35422083233621	13.408343912961767
16	14.025815645411416	15.786292857635381	50.94527121029597	19.242620286657232
17	14.662080583643963	16.10307329755864	30.42336090120858	38.81148521758882
18	19.812635794432072	21.036763025787252	38.25864048056526	20.891960699215417
19	28.677891213027074	23.147551001921233	27.042476701156932	21.132081083894764
20	32.27117919930127	21.01756421124645	26.06523000044617	20.646026589006112
21	20.970243189490947	28.334611000920734	29.540350635250917	21.154795174337405
22	22.327410093438736	23.32453162328681	26.31900587997496	28.02905240329949
23	17.779048685219212	30.982965784197212	25.860262374785197	25.377723155798382
24	18.816866293777014	22.011440871031287	43.081734220805295	16.0899586143864
25	16.15877690031083	23.704992638201045	39.09149046346209	21.04473999802604
26	15.745731981844951	34.87478181628898	28.278231383572034	21.101254818294038
27	15.959217391421914	34.89884793592463	30.061693092077245	19.080241580576207
28	13.399690926126476	27.151315051194587	42.45723193655198	16.991762086126965
29	13.954698909858863	22.22587270104336	41.28745627875597	22.531972110341805
30	16.731496466471704	30.9724199564917	33.73745485912532	18.558628717911276
31	29.26913357913224	26.164333740294122	25.20331138989953	19.363221290674108
32	29.491271975544496	25.105424476325293	27.07154532880674	18.331758219323472
33	27.421720889797452	27.838145881245868	25.630011803214853	19.110121425741823
34	18.537537062500252	27.06410916824516	28.711962348691035	25.686391420563552
35	18.84945019732866	24.80338115460589	33.32779001364197	23.019378634423475
36	30.611292959037574	23.55343016566414	26.820608710583276	19.01466816471501
37	19.26979607343682	32.57998266698575	29.627015706523135	18.5232055530543
38	19.278719466110715	32.21506998779118	26.10930615213844	22.396904393959673
39	18.981002637808956	32.03214043797634	27.536643371203674	21.450213553011036
40	24.21173317974282	25.780898261155254	25.649751429432865	24.357617129669062
41	16.61224749164784	24.008793597871364	28.287830790842435	31.091128119638363
42	21.4089766626241	25.615274685011	24.252023649694642	28.723725002670257
43	22.09621310143329	26.824664798162324	25.36947577772099	25.709646322683398
44	19.362680478996904	29.88660531158189	28.883129244526646	21.86758496489456
45	16.57750034138737	38.85191089045995	22.47410526088079	22.096483507271888
46	21.838381134325452	32.93489033015201	26.25289165243656	18.97383688308598
47	20.949962751595734	28.252678031824065	26.698520474454085	24.09883874212612
48	22.360129199909686	23.542208323362118	31.180091640538706	22.917570836189494
49	20.538134659403564	22.909864269789313	33.4808397182912	23.071161352515922
50	19.300892744876148	32.1984400287171	27.139822803053963	21.36084442335279
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	413.0
1	623.5
2	834.0
3	4526.5
4	8219.0
5	6245.5
6	4272.0
7	4566.0
8	4860.0
9	5185.5
10	5511.0
11	5531.5
12	5552.0
13	5236.5
14	4921.0
15	4595.5
16	4270.0
17	3960.0
18	3650.0
19	3468.0
20	3286.0
21	3412.5
22	3539.0
23	3549.5
24	3560.0
25	3999.5
26	4439.0
27	5351.0
28	6263.0
29	7950.5
30	9638.0
31	11817.0
32	13996.0
33	15406.0
34	16816.0
35	18921.0
36	21026.0
37	25185.0
38	29344.0
39	40584.0
40	51824.0
41	63692.0
42	75560.0
43	79263.0
44	82966.0
45	77998.0
46	73030.0
47	67076.5
48	61123.0
49	58400.5
50	55678.0
51	50380.5
52	45083.0
53	39341.5
54	33600.0
55	29370.5
56	25141.0
57	23114.5
58	21088.0
59	19706.5
60	18325.0
61	16143.0
62	13961.0
63	11980.0
64	9999.0
65	8395.0
66	6791.0
67	5764.0
68	4737.0
69	3747.0
70	2757.0
71	2257.5
72	1758.0
73	1326.5
74	895.0
75	677.5
76	460.0
77	351.0
78	242.0
79	183.5
80	125.0
81	82.0
82	39.0
83	29.0
84	19.0
85	12.0
86	5.0
87	7.0
88	9.0
89	6.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	739629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.748625650089934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.0821139763676	17.839332548682368
2	6.716033117834888	3.3242517897380397
3	3.125823353079027	2.320794960409852
4	2.1212214460702157	2.099892619589368
5	1.629726142689378	2.0166741108792237
6	1.2709641934874567	1.8872770223573723
7	1.1087234129856716	1.9207566488230716
8	0.9231049778181504	1.827646362540478
9	0.8787137434052598	1.957226174021336
>10	9.281327349843211	49.42906797629553
>50	0.7928589124461279	12.27469390487749
>100	0.06711431253130674	2.1528080615260414
>500	0.0	0.0
>1k	0.0022750614417392113	0.9495778202598846
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2677	0.3619382149699376	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1782	0.24093160219515458	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1172	0.15845782142128012	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1115	0.1507512550210984	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009599407270401783	0.0
2	0.0	0.0	0.0	0.038127223243004264	0.0
3	0.0	0.0	0.0	0.051782718092449054	0.0
4	0.0	0.0	0.0	0.07138714139115691	0.0
5	0.0	0.0	0.0	0.1228994536450031	0.0
6	0.0	0.0	0.0	0.20942932199792058	0.0
7	0.0	0.0	0.0	0.27310989698889576	0.0
8	0.0	0.0	0.0	0.6243670813340202	0.0
9	0.0	0.0	0.0	0.7599756093933581	0.0
10	0.0	0.0	0.0	0.984818064191642	0.0
11	0.0	0.0	0.0	1.1816735146945292	0.0
12	0.0	0.0	0.0	1.361493397365436	0.0
13	0.0	0.0	0.0	1.4336917562724014	0.0
14	0.0	0.0	0.0	1.4570818613115495	0.0
15	0.0	0.0	0.0	1.4972371283440753	0.0
16	0.0	0.0	0.0	1.5949888389990117	0.0
17	0.0	0.0	0.0	1.721673974384455	0.0
18	0.0	0.0	0.0	1.9252895708524138	0.0
19	0.0	0.0	0.0	1.996406306404968	0.0
20	0.0	0.0	0.0	2.073066361648881	0.0
21	0.0	0.0	0.0	2.175685377398669	0.0
22	0.0	0.0	0.0	2.2819548719695955	0.0
23	0.0	0.0	0.0	2.419997052576359	0.0
24	0.0	0.0	0.0	2.509366182234607	0.0
25	0.0	0.0	0.0	2.583862990769697	0.0
26	0.0	0.0	0.0	2.669716844526107	0.0
27	0.0	0.0	0.0	2.748675349398144	0.0
28	0.0	0.0	0.0	2.8333123768808415	0.0
29	0.0	0.0	0.0	2.9156509547354146	0.0
30	0.0	0.0	0.0	3.0427416988787623	0.0
31	0.0	0.0	0.0	3.1514448459971147	0.0
32	0.0	0.0	0.0	3.246357295346721	0.0
33	0.0	0.0	0.0	3.3434329914051504	0.0
34	0.0	0.0	0.0	3.439967875786374	0.0
35	0.0	0.0	0.0	3.589367101614458	0.0
36	0.0	0.0	0.0	3.694690175750275	0.0
37	0.0	0.0	0.0	3.8070438016897663	0.0
38	0.0	0.0	0.0	3.9157469488081187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	20	7.854659E-4	44.000004	17
CTCACCG	20	7.854659E-4	44.000004	42
GCGACTA	40	8.305506E-9	44.000004	1
CGTATTA	20	7.854659E-4	44.000004	35
TCACCCG	20	7.854659E-4	44.000004	32
ACGCATT	20	7.854659E-4	44.000004	14
CGCATCG	40	8.305506E-9	44.000004	21
TACGGGT	40	8.305506E-9	44.000004	4
TACGGGA	175	0.0	44.000004	4
TAACCGT	20	7.854659E-4	44.000004	40
GCCGACC	20	7.854659E-4	44.000004	14
TCGTGCG	20	7.854659E-4	44.000004	10
CTCGCGA	20	7.854659E-4	44.000004	1
ACCACGG	30	2.5264872E-6	44.000004	2
CGTGTAA	20	7.854659E-4	44.000004	2
ACCGTTA	20	7.854659E-4	44.000004	42
GCGTGTA	20	7.854659E-4	44.000004	1
AGGATCG	60	0.0	44.000004	7
CGCAAAA	20	7.854659E-4	44.000004	23
TTCGCGA	20	7.854659E-4	44.000004	15
>>END_MODULE