FastQCFastQC Report
Thu 26 May 2016
SRR1545763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545763_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences286009
Sequences flagged as poor quality0
Sequence length51
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7630.2667748217713429No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7500.26222951026016666No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6300.22027278861853997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC351.2076453E-745.00000425
CTCACGT351.2076453E-745.00000445
CGACGGT351.2076453E-745.00000428
AACCCGA253.883434E-545.00000424
GAGTATA253.883434E-545.0000041
CAACCCG253.883434E-545.00000423
AACGGGA502.1827873E-1145.0000044
GACGGTC351.2076453E-745.00000429
CGGTCTA351.2076453E-745.00000431
AATAGGC351.2076453E-745.0000044
ACGACGG351.2076453E-745.00000427
CTTCGGT207.024392E-445.034
GGTAATC406.7848305E-945.08
CGTATGG207.024392E-445.02
CGCGGGT207.024392E-445.029
AACTTCG207.024392E-445.011
CACGACG406.7848305E-945.026
CACGAAA207.024392E-445.035
GTAATCC207.024392E-445.09
CGGATCA207.024392E-445.031