##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545763_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	286009
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.998807729826684	31.0	31.0	33.0	30.0	33.0
2	31.150512746102397	31.0	31.0	33.0	30.0	34.0
3	31.079829655710135	31.0	31.0	34.0	28.0	34.0
4	35.029485086133654	35.0	35.0	37.0	32.0	37.0
5	32.727907163760584	35.0	35.0	37.0	28.0	37.0
6	33.94446328612037	35.0	35.0	37.0	28.0	37.0
7	15.637724686985374	0.0	0.0	35.0	0.0	37.0
8	25.101818474243817	17.0	17.0	35.0	17.0	37.0
9	32.84364128401554	32.0	32.0	37.0	27.0	39.0
10	35.57019883989665	37.0	35.0	37.0	32.0	39.0
11	36.46517766923419	37.0	35.0	39.0	32.0	39.0
12	36.17979504141478	37.0	35.0	39.0	32.0	39.0
13	35.70500229013772	37.0	35.0	39.0	31.0	39.0
14	36.745347174389615	39.0	35.0	40.0	31.0	41.0
15	37.205972539325685	39.0	35.0	41.0	32.0	41.0
16	37.31029792768759	39.0	35.0	41.0	32.0	41.0
17	37.28706089668507	39.0	35.0	41.0	32.0	41.0
18	37.1940638231664	39.0	35.0	40.0	32.0	41.0
19	37.09698645846809	39.0	35.0	40.0	32.0	41.0
20	37.17898737452318	39.0	35.0	40.0	32.0	41.0
21	36.97963700442993	39.0	35.0	40.0	32.0	41.0
22	37.04591813544329	39.0	35.0	40.0	32.0	41.0
23	37.04375386788528	39.0	35.0	40.0	32.0	41.0
24	36.86351478449979	38.0	35.0	40.0	32.0	41.0
25	36.62187553538525	38.0	35.0	40.0	31.0	41.0
26	36.499942309507745	38.0	35.0	40.0	31.0	41.0
27	36.37530287508435	38.0	35.0	40.0	31.0	41.0
28	36.34712194371506	38.0	35.0	40.0	31.0	41.0
29	36.39021499323448	38.0	35.0	40.0	31.0	41.0
30	36.14257593292519	38.0	35.0	40.0	30.0	41.0
31	35.98660531661591	38.0	34.0	40.0	30.0	41.0
32	35.829064819638546	38.0	34.0	40.0	30.0	41.0
33	35.58980661447717	38.0	34.0	40.0	29.0	41.0
34	35.43902464607757	38.0	34.0	40.0	27.0	41.0
35	35.258313549573614	38.0	34.0	40.0	27.0	41.0
36	35.020796548360366	38.0	34.0	40.0	25.0	41.0
37	34.86778388092682	38.0	34.0	40.0	25.0	41.0
38	34.80748158274739	38.0	34.0	40.0	24.0	41.0
39	34.749182718026354	38.0	34.0	40.0	24.0	41.0
40	34.59442884664469	37.0	33.0	40.0	23.0	41.0
41	34.472205420109155	37.0	33.0	40.0	23.0	41.0
42	34.434014314234865	37.0	33.0	40.0	23.0	41.0
43	34.34203119482254	37.0	33.0	40.0	23.0	41.0
44	34.12356254523459	37.0	33.0	40.0	23.0	41.0
45	34.02771591103777	37.0	33.0	40.0	23.0	41.0
46	34.02910747563888	37.0	33.0	40.0	23.0	41.0
47	33.98619274218643	36.0	33.0	40.0	23.0	41.0
48	33.99710498620673	36.0	33.0	40.0	23.0	41.0
49	33.92642539220794	36.0	33.0	40.0	23.0	41.0
50	33.78371659633088	36.0	33.0	40.0	23.0	41.0
51	33.545094734781074	36.0	33.0	40.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	6.0
14	0.0
15	14.0
16	30.0
17	75.0
18	199.0
19	359.0
20	665.0
21	1046.0
22	1571.0
23	2433.0
24	3342.0
25	4633.0
26	5255.0
27	5611.0
28	5968.0
29	6908.0
30	8356.0
31	11090.0
32	14491.0
33	22151.0
34	28647.0
35	28536.0
36	31547.0
37	48147.0
38	46162.0
39	8765.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.978364317206804	23.576880447818073	29.16027118027754	15.28448405469758
2	31.7615879220584	23.287728707837864	32.20702845015367	12.743654919950071
3	28.985451506770765	22.911167131104264	35.430353590271636	12.673027771853334
4	25.389061183389334	27.43689883884773	32.396532976235015	14.777507001527923
5	20.83186193441465	36.46318822134968	29.78192993926764	12.923019904968026
6	19.824201336321583	40.61795258191176	28.983004031341668	10.574842050424985
7	39.031289225164244	55.86397630843785	4.418742067557314	0.6859923988405959
8	88.75629787873808	2.697817201556594	7.264806352247656	1.2810785674576675
9	85.21095490002062	3.8673608173169374	8.658119150096676	2.263565132565758
10	54.151093147418436	23.235282805785832	13.734532829386486	8.879091217409242
11	49.113489435647125	18.843113328601547	20.8486446230713	11.19475261268002
12	44.82656140191393	20.54585694855756	23.423388774479125	11.204192875049387
13	20.309151110629386	43.989175165816455	23.508001496456405	12.193672227097748
14	13.710058075095539	45.34158016006489	27.269421591628234	13.678940173211332
15	11.58320192721208	22.91151677045128	52.17737903352692	13.327902268809721
16	14.054452831903891	15.997049043911204	50.65295148054781	19.29554664363709
17	14.658979262890329	16.466614686950408	29.918289284602935	38.95611676555633
18	19.744133925855483	21.183599117510287	38.19530154645483	20.876965410179398
19	29.584034068857974	23.121999657353438	26.48238342150072	20.811582852287867
20	32.827987930449744	21.32345485631571	25.525420528724617	20.32313668450993
21	21.01262547682066	28.877063309196565	28.975311965707373	21.134999248275406
22	22.151051190696798	23.53772084095256	25.683107874227733	28.62812009412291
23	17.650493515938308	31.519287854578003	25.411787740945215	25.418430888538474
24	18.440328800841932	22.224125814222628	43.35632794772192	15.979217437213514
25	16.11382858581373	23.971972909943393	39.110657356936315	20.803541147306554
26	15.76174176337108	35.52195909918919	27.78828638259635	20.92801275484338
27	15.81558622281117	35.9712456601016	29.39278134604156	18.820386771045666
28	13.389438793884109	27.608222118884374	42.047977511197196	16.954361576034323
29	13.796069354460874	22.6901950637917	40.98332569954092	22.530409882206502
30	16.686537836221937	32.04409651444535	32.97833284966557	18.291032799667143
31	29.89031813684185	26.303717715176795	24.84222524466013	18.96373890332122
32	30.338905419060243	24.79572321150733	26.790765325566678	18.074606043865753
33	28.227433402445378	27.630948676440255	24.956557311133565	19.185060609980805
34	18.528088276942334	26.94705411368174	28.19351838578506	26.331339223590867
35	18.95359936225783	24.734186686432942	33.38741088567143	22.924803065637793
36	31.246569163907427	23.81428556444028	26.411756273403984	18.527388998248306
37	19.2004447412494	33.581111083916944	29.128104360352296	18.090339814481364
38	19.04975018268656	33.15070504774325	25.655136726466647	22.14440804310354
39	19.02947110055977	32.7881290448902	27.149495295602588	21.032904558947447
40	24.74677370292543	25.60513829984371	25.359691478240194	24.28839651899066
41	16.650874622826553	23.917079532462264	27.873248743920648	31.558797100790535
42	20.93955085329483	25.504092528556793	24.062529500819902	29.493827117328475
43	21.85945197528749	26.36105856808702	25.3341678059082	26.445321650717286
44	18.850106115541816	30.021432891971934	29.42949347747798	21.69896751500827
45	16.535493638312083	39.74420385372488	22.187064043439193	21.533238464523844
46	21.68742941655682	33.74194518354317	25.81876794086899	18.75185745903101
47	20.58186980129996	28.427077469590117	26.37294630588548	24.618106423224447
48	21.9370719103245	24.030362680894658	31.5734819533651	22.459083455415737
49	20.42278389840879	23.447164250076046	33.7349523966029	22.395099454912256
50	19.23890506942089	33.28776367177257	26.671887947582068	20.80144331122447
51	16.983381641836445	35.2642749004402	24.295039666583918	23.457303791139438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	181.0
1	234.0
2	287.0
3	1489.0
4	2691.0
5	2051.5
6	1412.0
7	1537.0
8	1662.0
9	1713.0
10	1764.0
11	1801.0
12	1838.0
13	1731.0
14	1624.0
15	1569.0
16	1514.0
17	1382.0
18	1250.0
19	1220.0
20	1190.0
21	1287.5
22	1385.0
23	1392.0
24	1399.0
25	1616.5
26	2011.5
27	2189.0
28	2766.0
29	3343.0
30	3945.5
31	4548.0
32	5540.5
33	6533.0
34	7257.0
35	7981.0
36	9183.0
37	10385.0
38	13674.5
39	16964.0
40	21906.0
41	26848.0
42	29268.5
43	31689.0
44	30005.0
45	28321.0
46	26524.5
47	24728.0
48	23278.5
49	21829.0
50	20384.0
51	18939.0
52	16782.0
53	14625.0
54	12905.0
55	11185.0
56	9959.5
57	8734.0
58	8235.0
59	7736.0
60	6933.0
61	6130.0
62	5261.5
63	4393.0
64	3758.5
65	3124.0
66	2633.5
67	2143.0
68	1763.0
69	1383.0
70	1187.5
71	992.0
72	810.0
73	628.0
74	454.5
75	220.5
76	160.0
77	122.5
78	85.0
79	65.0
80	45.0
81	28.5
82	12.0
83	11.0
84	10.0
85	8.5
86	7.0
87	6.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	286009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.75383292134163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.65101150830235	23.17234772332339
2	8.301308279555412	5.604019453933268
3	4.442764064264184	4.498809478023419
4	3.0122541149172877	4.067004884461678
5	2.3389510974839185	3.9474282277830417
6	1.7474802929386053	3.539049470471209
7	1.4936968479060275	3.529259568754829
8	1.233698298097142	3.331363698345157
9	1.0317073928671314	3.1341671066295116
>10	7.680833652720661	43.017877059812804
>50	0.0590434953749262	1.234226894957851
>100	0.0041434031842053475	0.1751693128537913
>500	0.00310755238815401	0.7492771206500495
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	763	0.2667748217713429	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	750	0.26222951026016666	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	630	0.22027278861853997	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007692065634298221	0.0
2	0.0	0.0	0.0	0.043355279029680886	0.0
3	0.0	0.0	0.0	0.0541940987871011	0.0
4	0.0	0.0	0.0	0.08216524654818555	0.0
5	0.0	0.0	0.0	0.1374082633763273	0.0
6	0.0	0.0	0.0	0.20488865734994352	0.0
7	0.0	0.0	0.0	0.25034177246170575	0.0
8	0.0	0.0	0.0	0.5297036107255366	0.0
9	0.0	0.0	0.0	0.6531263002213217	0.0
10	0.0	0.0	0.0	0.8527703673660619	0.0
11	0.0	0.0	0.0	1.0104577128691754	0.0
12	0.0	0.0	0.0	1.1566069599208417	0.0
13	0.0	0.0	0.0	1.2149967308721055	0.0
14	0.0	0.0	0.0	1.2356254523459052	0.0
15	0.0	0.0	0.0	1.2803792887636403	0.0
16	0.0	0.0	0.0	1.3688380435580698	0.0
17	0.0	0.0	0.0	1.47407948700915	0.0
18	0.0	0.0	0.0	1.6506473572509957	0.0
19	0.0	0.0	0.0	1.7170788331835711	0.0
20	0.0	0.0	0.0	1.779314636951984	0.0
21	0.0	0.0	0.0	1.8698712278284948	0.0
22	0.0	0.0	0.0	1.9513371956826533	0.0
23	0.0	0.0	0.0	2.0740606064844114	0.0
24	0.0	0.0	0.0	2.1618200825848137	0.0
25	0.0	0.0	0.0	2.227552279823362	0.0
26	0.0	0.0	0.0	2.307270050942453	0.0
27	0.0	0.0	0.0	2.368456936669825	0.0
28	0.0	0.0	0.0	2.4380351667255225	0.0
29	0.0	0.0	0.0	2.5170536591505863	0.0
30	0.0	0.0	0.0	2.6153023156613955	0.0
31	0.0	0.0	0.0	2.718795562377408	0.0
32	0.0	0.0	0.0	2.7999118908845526	0.0
33	0.0	0.0	0.0	2.888370645678982	0.0
34	0.0	0.0	0.0	2.9785775972084796	0.0
35	0.0	0.0	0.0	3.1114405490736305	0.0
36	0.0	0.0	0.0	3.2128359597075615	0.0
37	0.0	0.0	0.0	3.3215737966287775	0.0
38	0.0	0.0	0.0	3.421920289221668	0.0
39	0.0	0.0	0.0	3.508281207934016	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	35	1.2076453E-7	45.000004	25
CTCACGT	35	1.2076453E-7	45.000004	45
CGACGGT	35	1.2076453E-7	45.000004	28
AACCCGA	25	3.883434E-5	45.000004	24
GAGTATA	25	3.883434E-5	45.000004	1
CAACCCG	25	3.883434E-5	45.000004	23
AACGGGA	50	2.1827873E-11	45.000004	4
GACGGTC	35	1.2076453E-7	45.000004	29
CGGTCTA	35	1.2076453E-7	45.000004	31
AATAGGC	35	1.2076453E-7	45.000004	4
ACGACGG	35	1.2076453E-7	45.000004	27
CTTCGGT	20	7.024392E-4	45.0	34
GGTAATC	40	6.7848305E-9	45.0	8
CGTATGG	20	7.024392E-4	45.0	2
CGCGGGT	20	7.024392E-4	45.0	29
AACTTCG	20	7.024392E-4	45.0	11
CACGACG	40	6.7848305E-9	45.0	26
CACGAAA	20	7.024392E-4	45.0	35
GTAATCC	20	7.024392E-4	45.0	9
CGGATCA	20	7.024392E-4	45.0	31
>>END_MODULE