FastQCFastQC Report
Thu 26 May 2016
SRR1545762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545762_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1654012
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75030.45362427842119646No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32190.1946176932210891No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19000.11487220165270869No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCGAA302.5283935E-644.0000041
CTTAACG207.857486E-444.01
TAGCGAT254.44369E-544.06
CGTTTTA39300.043.1603051
CGGTCTA4300.041.95348731
CGTTATT21200.041.405661
TTTACGG1700.040.117652
CGACGGT4500.040.0888928
ACGGTCT4500.040.0888930
AGGCGAT13950.040.0573467
GTTTTAT47350.039.446672
CGCATCG1400.039.28571321
CACGACG4600.039.21739226
TCACGAC4700.038.85106725
TTACTGG27500.038.840
GCGCGAC11900.038.6386579
GTTATTT24500.038.6122442
GGCGTAA404.1259955E-738.51
TGATTCG1150.038.2608715
TCAGACG12550.038.0398422
Produced by FastQC (version 0.11.5)