##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545762_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1654012
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.476065469899854	31.0	31.0	33.0	30.0	34.0
2	31.67104289448928	31.0	31.0	34.0	30.0	34.0
3	31.72155280614651	31.0	31.0	34.0	30.0	34.0
4	35.643057003214004	37.0	35.0	37.0	33.0	37.0
5	26.99315240760043	35.0	22.0	37.0	0.0	37.0
6	31.269838429225423	35.0	25.0	37.0	17.0	37.0
7	15.134691888571545	0.0	0.0	32.0	0.0	37.0
8	25.153931773167304	17.0	17.0	35.0	17.0	37.0
9	33.1191025216262	32.0	32.0	37.0	28.0	39.0
10	35.929042231858055	37.0	35.0	39.0	32.0	39.0
11	36.93528583831314	38.0	37.0	39.0	34.0	39.0
12	37.07755385087896	39.0	37.0	39.0	34.0	39.0
13	36.719917388749295	39.0	35.0	39.0	33.0	39.0
14	38.04948271233824	40.0	37.0	41.0	33.0	41.0
15	38.28181355395245	40.0	37.0	41.0	33.0	41.0
16	38.30842158339843	40.0	37.0	41.0	34.0	41.0
17	38.32261313702682	40.0	37.0	41.0	34.0	41.0
18	38.28986065397349	40.0	37.0	41.0	34.0	41.0
19	38.24427634140502	40.0	37.0	41.0	34.0	41.0
20	38.28602452702882	40.0	37.0	41.0	34.0	41.0
21	38.164187442412754	40.0	37.0	41.0	34.0	41.0
22	38.06934713895667	40.0	36.0	41.0	34.0	41.0
23	37.96530980428195	40.0	36.0	41.0	34.0	41.0
24	37.80395003180146	40.0	35.0	41.0	33.0	41.0
25	37.62570465026856	39.0	35.0	41.0	33.0	41.0
26	37.37612907282414	39.0	35.0	40.0	33.0	41.0
27	37.19136983286699	39.0	35.0	40.0	33.0	41.0
28	37.18777312377419	39.0	35.0	40.0	32.0	41.0
29	37.08643468124778	39.0	35.0	40.0	32.0	41.0
30	36.88668340979388	39.0	35.0	40.0	32.0	41.0
31	36.732370139998984	39.0	35.0	40.0	31.0	41.0
32	36.54193198114645	39.0	35.0	40.0	31.0	41.0
33	36.33834276897628	38.0	35.0	40.0	30.0	41.0
34	36.07277395810913	38.0	35.0	40.0	30.0	41.0
35	35.87106320873126	38.0	35.0	40.0	29.0	41.0
36	35.69816180293734	38.0	35.0	40.0	29.0	41.0
37	35.585444361951424	38.0	35.0	40.0	28.0	41.0
38	35.3879597004133	38.0	34.0	40.0	27.0	41.0
39	35.29788236119206	38.0	34.0	40.0	27.0	41.0
40	35.054634428287095	38.0	34.0	40.0	26.0	41.0
41	34.83144015883803	38.0	34.0	40.0	25.0	41.0
42	34.817364686592356	38.0	34.0	40.0	25.0	41.0
43	34.692515531930844	37.0	34.0	40.0	25.0	41.0
44	34.474412519377125	37.0	34.0	40.0	24.0	40.0
45	34.28737336851244	37.0	33.0	40.0	24.0	40.0
46	34.21385032273043	37.0	33.0	40.0	24.0	40.0
47	34.03499611852877	36.0	33.0	39.0	23.0	40.0
48	33.89781815367724	36.0	33.0	39.0	23.0	40.0
49	33.756062229294585	36.0	33.0	39.0	24.0	40.0
50	33.84603981107755	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	3.0
12	5.0
13	7.0
14	12.0
15	41.0
16	114.0
17	278.0
18	604.0
19	1233.0
20	2262.0
21	3864.0
22	5944.0
23	9189.0
24	14057.0
25	20174.0
26	25840.0
27	28449.0
28	29824.0
29	33439.0
30	40500.0
31	52252.0
32	71285.0
33	116266.0
34	131499.0
35	171227.0
36	241553.0
37	422237.0
38	182121.0
39	49731.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.83643891338152	22.443247086478213	26.552044362435094	15.168269637705167
2	36.249011494475255	23.159142738988592	28.150763114173294	12.44108265236286
3	31.91814811500763	23.552005668640856	31.661559891947576	12.868286324403936
4	28.48479938476867	25.693465343661355	30.017315472922807	15.804419798647167
5	17.35966849091784	48.225284943519156	22.63562779471975	11.77941877084326
6	20.68564194213827	41.02279789989432	28.55728978991688	9.734270368050533
7	39.25322186296109	55.36332263611147	4.271734425143228	1.1117210757842144
8	85.4918827674769	3.351184876530521	8.88723902849556	2.2696933274970195
9	80.51616312336307	5.299115121292953	10.58232951151503	3.6023922438289446
10	42.566740749160225	26.324718321269735	16.92279137031654	14.1857495592535
11	36.222288592827624	24.275095948517908	23.469116306290402	16.03349915236407
12	32.3579877292305	22.000203142419764	29.068047873896923	16.57376125445281
13	23.833442562689992	28.874397525531858	29.52390913729767	17.768250774480478
14	19.337707344323984	32.97648384655009	30.744093755063446	16.941715054062488
15	18.552646534607973	24.19166245468594	39.31017429135943	17.945516719346656
16	20.968469394417937	19.79344768961773	39.16283557797646	20.075247337987875
17	21.010246600387422	20.132925275028235	30.664227345388063	28.19260077919628
18	23.198320205657517	23.34384514743545	32.54256921957035	20.91526542733668
19	27.726098722379284	24.727934259243582	27.24393777070541	20.302029247671722
20	29.018531909079258	24.175338510240554	25.771457522678194	21.034672058001995
21	25.6912283586818	25.51384149570862	28.98020086915935	19.814729276450233
22	24.233258283494923	22.89064408238876	28.081658416021167	24.794439218095153
23	22.441614692033674	27.42773329334975	27.021508912873664	23.109143101742912
24	22.079888174934645	25.921335516308226	32.505870574094985	19.49290573466214
25	22.95334012086974	23.60732570259466	31.595054933096012	21.844279243439587
26	20.346708488209273	28.351970844226038	28.16926358454473	23.132057083019955
27	21.55552680391678	26.910143336324044	30.226201502770234	21.308128356988945
28	20.299187672157153	26.21831038710723	34.06643966307379	19.41606227766183
29	21.024031264585748	25.98856598380181	32.72908539962226	20.25831735199019
30	22.710657480115017	25.514990217725142	31.140826064139798	20.63352623802004
31	27.730995905712895	24.719893205127896	26.752828879113334	20.79628201004588
32	26.942307552786797	25.73663310786137	27.345871734908815	19.975187604443015
33	26.170124521466594	25.190627395689997	27.39442035487046	21.244827727972954
34	21.852985347143793	26.531246448030604	29.294285652099262	22.32148255272634
35	22.54747849471467	24.50967707610344	29.71622938648571	23.22661504269618
36	27.499316812695433	24.99873035987647	26.610568726224475	20.89138410120362
37	22.73266457558954	28.790540818325383	27.055245064727462	21.42154954135762
38	23.179154685697565	29.080200143650707	24.844982986822345	22.89566218382938
39	22.790342512629895	26.49563606551827	26.32211858196918	24.39190283988266
40	23.82642931248383	24.599700606767062	27.76666674727874	23.807203333470373
41	21.108794857594745	24.367840136589095	28.22216525635848	26.30119974945768
42	23.12298822499474	27.363223483263727	24.99613062057591	24.517657671165626
43	22.604733218380517	26.276411537522097	26.426531367366135	24.692323876731244
44	22.39475892557007	27.152523681811257	28.63969547983932	21.81302191277935
45	21.549359980459634	29.03255840949159	26.423085201316553	22.99499640873222
46	23.61240426308878	27.54163815014643	27.774163669912916	21.07179391685187
47	23.49656471658005	26.84738683878956	27.468603613516713	22.18744483111368
48	22.385448231330848	26.65004848816091	28.461945862545136	22.50255741796311
49	22.49445590479392	25.392076961956743	29.69343632331567	22.420030809933664
50	21.18787529957461	27.769266486579298	28.507592447938706	22.53526576590738
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	718.0
1	1686.5
2	2655.0
3	12897.0
4	23139.0
5	16620.0
6	10101.0
7	9960.0
8	9819.0
9	9881.0
10	9943.0
11	9690.0
12	9437.0
13	9196.5
14	8956.0
15	8569.5
16	8183.0
17	7932.5
18	7682.0
19	7498.5
20	7315.0
21	7563.0
22	7811.0
23	8776.0
24	9741.0
25	11340.5
26	12940.0
27	15455.5
28	17971.0
29	20549.5
30	23128.0
31	26637.0
32	30146.0
33	33041.0
34	35936.0
35	38890.5
36	41845.0
37	47801.0
38	53757.0
39	67308.0
40	80859.0
41	92649.5
42	104440.0
43	113792.0
44	123144.0
45	127922.0
46	132700.0
47	134158.0
48	135616.0
49	131047.5
50	126479.0
51	118406.0
52	110333.0
53	102425.0
54	94517.0
55	90809.0
56	87101.0
57	83925.0
58	80749.0
59	75414.0
60	70079.0
61	62097.5
62	54116.0
63	47260.5
64	40405.0
65	34380.0
66	28355.0
67	24458.0
68	20561.0
69	17481.0
70	14401.0
71	11824.5
72	9248.0
73	7070.0
74	4892.0
75	3653.0
76	2414.0
77	1836.0
78	1258.0
79	946.5
80	635.0
81	462.0
82	289.0
83	210.5
84	132.0
85	81.0
86	30.0
87	24.5
88	19.0
89	12.5
90	6.0
91	5.5
92	5.0
93	4.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1654012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.00342304673563
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.40745768609916	20.276566693224442
2	7.072529486880247	3.961100704632393
3	3.4627863734788837	2.9090961521100196
4	2.2948176627961376	2.570509993056054
5	1.7627593761212197	2.468164826956114
6	1.3472938276592037	2.2637303414517844
7	1.1343952723887072	2.223686550044251
8	0.9599041421645976	2.150448142187927
9	0.8597841579089195	2.1669209552524342
>10	8.223407246027485	47.398110064122235
>50	0.42231742712969333	7.366296587964216
>100	0.046317501969315514	2.239343024282264
>500	0.005417251631756422	1.0486372353083224
>1k	5.417251631756422E-4	0.3882802175438193
>5k	2.708625815878211E-4	0.5691085118638946
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7503	0.45362427842119646	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3219	0.1946176932210891	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1900	0.11487220165270869	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0074364635806753515	0.0
2	0.0	0.0	0.0	0.026057852059114445	0.0
3	0.0	0.0	0.0	0.036094054940351096	0.0
4	0.0	0.0	0.0	0.053143508027753125	0.0
5	0.0	0.0	0.0	0.08972123539611562	0.0
6	0.0	0.0	0.0	0.18554883519587523	0.0
7	0.0	0.0	0.0	0.2518724168869392	0.0
8	0.0	0.0	0.0	0.6350014389254733	0.0
9	0.0	0.0	0.0	0.816197222269246	0.0
10	0.0	0.0	0.0	1.0888675535606755	0.0
11	0.0	0.0	0.0	1.2736908801145337	0.0
12	0.0	0.0	0.0	1.447873413252141	0.0
13	0.0	0.0	0.0	1.5253819198409686	0.0
14	0.0	0.0	0.0	1.5577275134642312	0.0
15	0.0	0.0	0.0	1.5968445210796536	0.0
16	0.0	0.0	0.0	1.6858402478337522	0.0
17	0.0	7.255086420171075E-4	0.0	1.78880201594668	0.0
18	0.0	7.255086420171075E-4	0.0	1.9431539795358195	0.0
19	0.0	7.255086420171075E-4	0.0	2.007845166782345	0.0
20	0.0	7.255086420171075E-4	0.0	2.0840840332476427	0.0
21	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.180093010207907	0.0
22	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.28118054766229	0.0
23	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.3947226501379677	0.0
24	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.4816627690730177	0.0
25	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.552278943562683	0.0
26	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.6300897454190175	0.0
27	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.7032452001557425	0.0
28	6.0459053501425626E-5	7.255086420171075E-4	0.0	2.7861950215596987	0.0
29	1.2091810700285125E-4	7.255086420171075E-4	0.0	2.8776695695073555	0.0
30	1.2091810700285125E-4	7.255086420171075E-4	0.0	2.996108855316648	0.0
31	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.09882878721557	0.0
32	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.201065046686481	0.0
33	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.3089844571865257	0.0
34	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.4158760637770462	0.0
35	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.5445933886815815	0.0
36	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.654024275519162	0.0
37	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.7647852615337736	0.0
38	1.2091810700285125E-4	7.255086420171075E-4	0.0	3.8987020650394313	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCGAA	30	2.5283935E-6	44.000004	1
CTTAACG	20	7.857486E-4	44.0	1
TAGCGAT	25	4.44369E-5	44.0	6
CGTTTTA	3930	0.0	43.160305	1
CGGTCTA	430	0.0	41.953487	31
CGTTATT	2120	0.0	41.40566	1
TTTACGG	170	0.0	40.11765	2
CGACGGT	450	0.0	40.08889	28
ACGGTCT	450	0.0	40.08889	30
AGGCGAT	1395	0.0	40.057346	7
GTTTTAT	4735	0.0	39.44667	2
CGCATCG	140	0.0	39.285713	21
CACGACG	460	0.0	39.217392	26
TCACGAC	470	0.0	38.851067	25
TTACTGG	2750	0.0	38.8	40
GCGCGAC	1190	0.0	38.638657	9
GTTATTT	2450	0.0	38.612244	2
GGCGTAA	40	4.1259955E-7	38.5	1
TGATTCG	115	0.0	38.26087	15
TCAGACG	1255	0.0	38.03984	22
>>END_MODULE