FastQCFastQC Report
Thu 26 May 2016
SRR1545761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545761_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences637377
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19160.3006070190797597No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.28931072191183554No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7500.11766976216587673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT351.2107921E-745.09
CGATTCA302.1639735E-644.99999610
CGTTTTA11350.043.8105741
CGTTTTT15650.042.9872171
AGGCGAT6150.041.7073177
CGACGGT1350.041.66666828
CGGTCTA1350.041.66666831
ACGGTCT1350.041.66666830
CAAGGAC5250.039.8571435
ACACGAC403.4549885E-739.37526
GACACGA403.4549885E-739.37525
CACGACC403.4549885E-739.37527
GTTTTAT14350.039.198612
AATAGGC750.039.04
CACGACG1450.038.79310226
GACGGTC1450.038.79310229
GCAAGGA7950.038.7735864
ACGTTAG356.2439103E-638.5714262
ATAACGC356.2439103E-638.57142611
CGTTTAG356.2439103E-638.5714262
Produced by FastQC (version 0.11.5)