##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545761_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	637377
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.938017845011665	31.0	31.0	33.0	30.0	33.0
2	31.109652529036975	31.0	31.0	33.0	30.0	34.0
3	31.037751597563137	31.0	31.0	34.0	28.0	34.0
4	35.037699822867786	35.0	35.0	37.0	32.0	37.0
5	32.711178784298774	35.0	35.0	37.0	28.0	37.0
6	33.91845171695872	35.0	35.0	37.0	28.0	37.0
7	15.751981951027414	0.0	0.0	35.0	0.0	37.0
8	25.11848874371055	17.0	17.0	35.0	17.0	37.0
9	32.658158358396996	32.0	32.0	37.0	27.0	39.0
10	35.37806510118815	37.0	34.0	37.0	32.0	39.0
11	36.35101674519162	37.0	35.0	39.0	32.0	39.0
12	36.350947712264485	37.0	35.0	39.0	32.0	39.0
13	36.21816444584602	38.0	35.0	39.0	32.0	39.0
14	37.25016748329482	39.0	36.0	40.0	32.0	41.0
15	37.517006418493295	39.0	36.0	41.0	32.0	41.0
16	37.47395340591204	39.0	36.0	41.0	32.0	41.0
17	37.41469020689482	39.0	36.0	41.0	32.0	41.0
18	37.33304621911365	39.0	36.0	40.0	32.0	41.0
19	37.29353585083867	39.0	36.0	40.0	32.0	41.0
20	37.41910831423161	39.0	36.0	40.0	32.0	41.0
21	37.2915291891612	39.0	35.0	40.0	32.0	41.0
22	37.33621859590164	39.0	35.0	41.0	32.0	41.0
23	37.29644150949909	39.0	35.0	40.0	32.0	41.0
24	37.09869825864441	39.0	35.0	40.0	32.0	41.0
25	36.84394322355529	39.0	35.0	40.0	31.0	41.0
26	36.72909439782107	39.0	35.0	40.0	31.0	41.0
27	36.664018312552855	38.0	35.0	40.0	31.0	41.0
28	36.539766888356496	38.0	35.0	40.0	31.0	41.0
29	36.47985885904261	38.0	35.0	40.0	30.0	41.0
30	36.19922118306748	38.0	35.0	40.0	30.0	41.0
31	36.08623153957548	38.0	35.0	40.0	30.0	41.0
32	35.99973798866291	38.0	35.0	40.0	30.0	41.0
33	35.81963265069182	38.0	35.0	40.0	29.0	41.0
34	35.63407998719753	38.0	34.0	40.0	27.0	41.0
35	35.44543025556303	38.0	34.0	40.0	26.0	41.0
36	35.25962656324279	38.0	34.0	40.0	25.0	41.0
37	35.119497565804856	38.0	34.0	40.0	25.0	41.0
38	34.960174276762416	38.0	34.0	40.0	24.0	41.0
39	34.83095091288202	38.0	34.0	40.0	24.0	41.0
40	34.68947891122522	38.0	33.0	40.0	23.0	41.0
41	34.504081571817	38.0	33.0	40.0	23.0	41.0
42	34.447587534536076	38.0	33.0	40.0	23.0	41.0
43	34.36070488894328	37.0	33.0	40.0	23.0	41.0
44	34.14093856540164	37.0	33.0	40.0	23.0	41.0
45	34.062211218792015	37.0	33.0	40.0	23.0	41.0
46	34.029210341760056	37.0	33.0	40.0	23.0	41.0
47	33.946714424900804	37.0	33.0	40.0	23.0	41.0
48	33.83976516253332	37.0	33.0	40.0	22.0	41.0
49	33.71309758588716	36.0	33.0	40.0	22.0	41.0
50	33.598666095576085	36.0	32.0	40.0	21.0	41.0
51	33.425446792086944	36.0	32.0	40.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	14.0
15	36.0
16	89.0
17	224.0
18	448.0
19	837.0
20	1432.0
21	2280.0
22	3480.0
23	5219.0
24	7194.0
25	9705.0
26	11422.0
27	12724.0
28	13803.0
29	15592.0
30	19050.0
31	23650.0
32	30679.0
33	43107.0
34	55473.0
35	63721.0
36	79591.0
37	116552.0
38	98427.0
39	22626.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.52183401660242	23.28951311390276	26.62678446194325	14.561868407551575
2	36.15521112308728	22.840014622429113	28.582769695172562	12.422004559311052
3	32.56565580496316	23.168548598396242	31.187821336508847	13.077974260131759
4	29.15213445103918	26.317862113003766	28.581828337075233	15.948175098881823
5	21.356904940090402	36.56062267700278	27.528291733150084	14.554180649756738
6	20.63174541911616	41.83583656140714	28.09702891695182	9.43538910252488
7	39.00752615798813	55.57276148966781	4.558840372338506	0.860871980005554
8	85.92685333797738	3.649488450320611	8.243472858292659	2.180185353409364
9	80.9362433849982	5.440108444452812	9.931641712832437	3.692006457716548
10	42.31969462343323	27.201954259410048	16.336642207045436	14.141708910111284
11	36.37580270389424	24.838988542103024	22.938072757567344	15.84713599643539
12	32.638297271473554	22.462059346352316	28.50918687056483	16.3904565116093
13	23.980156171308348	29.54373941952722	28.799282057557775	17.676822351606663
14	19.46320623430089	33.742824105670586	30.108083598874764	16.68588606115376
15	18.541930443050187	24.743911374272997	38.89707347456843	17.81708470810839
16	20.92984214993638	20.328157432728197	38.74598549994744	19.99601491738798
17	20.89752218859482	20.541218148756542	30.22434132389465	28.336918338753986
18	23.172157137769325	23.796277556297138	32.22143252737391	20.810132778559627
19	28.053883337490998	24.98081982876696	26.714016978962213	20.251279854779824
20	29.378687966462547	24.536655699844832	25.163913978697067	20.920742354995554
21	25.853772571021544	26.072324542617636	28.415678632897013	19.658224253463803
22	24.256915451922488	23.31744007079013	27.443883290423095	24.98176118686429
23	22.456881876817018	28.02862356187939	26.386110575059973	23.12838398624362
24	21.962355089687893	26.44776953043489	32.278855371310854	19.31102000856636
25	23.005536754542444	23.97670452495148	31.341890278438033	21.67586844206804
26	20.32690228859843	28.884004286317204	27.720014998972353	23.069078426112018
27	21.54690238273424	27.686596786517242	29.66831247440683	21.098188356341694
28	20.258496933526	26.85004322402597	33.71066103734525	19.18079880510279
29	20.93235243819592	26.53986573095672	32.47057863713312	20.057203193714237
30	22.578944643437087	26.11719594525689	30.876388699309825	20.427470711996197
31	28.112875111590157	25.07354360135367	26.32209822444174	20.491483062614435
32	27.28369552086128	26.049575055265567	26.97116463254871	19.695564791324443
33	26.446357493288904	25.457147026014432	26.895071519681444	21.201423961015223
34	21.8586488059657	26.766733032412525	28.847918892586332	22.52669926903544
35	22.596359768237637	24.64773595532942	29.56994055323615	23.18596372319679
36	27.93856697056844	25.302607405036582	26.182149653972452	20.576675970422528
37	22.626012548303436	29.34134742860191	26.718253090400186	21.314386932694465
38	22.859312463424317	29.82489170459555	24.53336094650419	22.782434885475944
39	22.672766667137346	27.087108571536156	26.00690015485341	24.23322460647309
40	23.894492584451587	24.883389265693616	27.574418279919104	23.64769986993569
41	20.982871989419134	24.676447377297894	28.115542292865918	26.225138340417054
42	23.043347971451748	27.604227953001125	24.707512194509686	24.64491188103744
43	22.50661696295913	26.390660472530385	26.09287752774261	25.00984503676788
44	22.189849963208587	27.35523873625813	28.78594615117897	21.668965149354307
45	21.448216675531125	29.684943134126268	26.258556552872165	22.608283637470443
46	23.665585673784904	28.100951242357347	27.354768057209473	20.878695026648277
47	23.482177737822358	27.276321549098885	27.053376573048602	22.188124140030155
48	22.172277945391816	27.059181614648786	28.381005276312138	22.38753516364726
49	22.31928670159105	25.86224479389749	29.790845920075558	22.0276225844359
50	21.27892911102848	28.343664738451498	28.15335350977522	22.2240526407448
51	21.734075751086092	29.485218324476726	26.54833795383266	22.232367970604525
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	623.0
2	935.0
3	4161.0
4	7387.0
5	5373.0
6	3359.0
7	3299.0
8	3239.0
9	3300.5
10	3362.0
11	3346.0
12	3330.0
13	3172.0
14	3014.0
15	2974.0
16	2934.0
17	2834.5
18	2735.0
19	2753.0
20	2771.0
21	2828.0
22	2885.0
23	3287.0
24	3689.0
25	4089.0
26	5539.0
27	6589.0
28	7566.0
29	8543.0
30	9606.5
31	10670.0
32	12047.5
33	13425.0
34	14680.0
35	15935.0
36	17590.0
37	19245.0
38	23567.5
39	27890.0
40	33107.0
41	38324.0
42	41530.5
43	44737.0
44	46629.5
45	48522.0
46	49856.5
47	51191.0
48	49600.0
49	48009.0
50	46468.0
51	44927.0
52	42487.0
53	40047.0
54	37348.5
55	34650.0
56	33055.0
57	31460.0
58	29687.5
59	27915.0
60	25530.5
61	23146.0
62	20374.0
63	17602.0
64	15204.0
65	12806.0
66	10981.0
67	9156.0
68	7934.5
69	6713.0
70	5824.5
71	4936.0
72	4061.0
73	3186.0
74	2488.0
75	1270.0
76	750.0
77	563.5
78	377.0
79	291.5
80	206.0
81	155.5
82	105.0
83	76.5
84	48.0
85	34.0
86	20.0
87	15.0
88	10.0
89	6.0
90	2.0
91	3.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	637377.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.41872359623458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.80694374771119	25.372492860010315
2	9.193596323957387	6.880252794030395
3	5.074507733716284	5.696448068246531
4	3.542250417106972	5.301859570654897
5	2.5987000359916443	4.862001917814809
6	2.071019598950318	4.649694596130393
7	1.6683158766760378	4.3698375462385295
8	1.3139384510264946	3.933271977714
9	1.106872039025315	3.727596499621995
>10	5.561057756682992	31.819804486900626
>50	0.03793168957108259	0.9653193811994307
>100	0.023601939944362185	1.7101655431024199
>500	4.214632132921818E-4	0.11827961613120758
>1k	8.429264265843636E-4	0.5929751422044539
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1916	0.3006070190797597	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1844	0.28931072191183554	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	750	0.11766976216587673	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006589506681289096	0.0
2	0.0	0.0	0.0	0.026358026725156383	0.0
3	0.0	0.0	0.0	0.03624228674709003	0.0
4	0.0	0.0	0.0	0.05601080679095732	0.0
5	0.0	0.0	0.0	0.09884260021933644	0.0
6	0.0	0.0	0.0	0.16850309942153546	0.0
7	0.0	0.0	0.0	0.21478653920678029	0.0
8	0.0	0.0	0.0	0.5539892402769475	0.0
9	0.0	0.0	0.0	0.7099408984007894	0.0
10	0.0	0.0	0.0	0.9303755861915318	0.0
11	0.0	0.0	0.0	1.0869548163802585	0.0
12	0.0	0.0	0.0	1.2372583259201384	0.0
13	0.0	0.0	0.0	1.3004862114572695	0.0
14	0.0	0.0	0.0	1.3306096705717338	0.0
15	0.0	0.0	0.0	1.3590073065077655	0.0
16	0.0	0.0	0.0	1.4344728473101478	0.0
17	0.0	9.413580973270136E-4	0.0	1.5265690478319738	0.0
18	0.0	9.413580973270136E-4	0.0	1.6674589763985836	0.0
19	0.0	9.413580973270136E-4	0.0	1.7219008530273292	0.0
20	0.0	9.413580973270136E-4	0.0	1.7846580595157968	0.0
21	0.0	9.413580973270136E-4	0.0	1.8660855349345835	0.0
22	0.0	9.413580973270136E-4	0.0	1.950023298612909	0.0
23	0.0	9.413580973270136E-4	0.0	2.0499641499457937	0.0
24	0.0	9.413580973270136E-4	0.0	2.128410658056378	0.0
25	0.0	9.413580973270136E-4	0.0	2.190069613431297	0.0
26	0.0	9.413580973270136E-4	0.0	2.254238857065755	0.0
27	0.0	9.413580973270136E-4	0.0	2.3213890680084157	0.0
28	0.0	9.413580973270136E-4	0.0	2.398580431989231	0.0
29	0.0	9.413580973270136E-4	0.0	2.474830437872719	0.0
30	0.0	9.413580973270136E-4	0.0	2.5793211866760175	0.0
31	0.0	9.413580973270136E-4	0.0	2.6806740751548928	0.0
32	0.0	9.413580973270136E-4	0.0	2.7716720245631707	0.0
33	0.0	9.413580973270136E-4	0.0	2.8727111270096035	0.0
34	0.0	9.413580973270136E-4	0.0	2.972338192310046	0.0
35	0.0	9.413580973270136E-4	0.0	3.0920475636867977	0.0
36	0.0	9.413580973270136E-4	0.0	3.197950349636087	0.0
37	0.0	9.413580973270136E-4	0.0	3.3018135263745005	0.0
38	0.0	9.413580973270136E-4	0.0	3.423091827913464	0.0
39	0.0	9.413580973270136E-4	0.0	3.600537829259606	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAT	35	1.2107921E-7	45.0	9
CGATTCA	30	2.1639735E-6	44.999996	10
CGTTTTA	1135	0.0	43.810574	1
CGTTTTT	1565	0.0	42.987217	1
AGGCGAT	615	0.0	41.707317	7
CGACGGT	135	0.0	41.666668	28
CGGTCTA	135	0.0	41.666668	31
ACGGTCT	135	0.0	41.666668	30
CAAGGAC	525	0.0	39.857143	5
ACACGAC	40	3.4549885E-7	39.375	26
GACACGA	40	3.4549885E-7	39.375	25
CACGACC	40	3.4549885E-7	39.375	27
GTTTTAT	1435	0.0	39.19861	2
AATAGGC	75	0.0	39.0	4
CACGACG	145	0.0	38.793102	26
GACGGTC	145	0.0	38.793102	29
GCAAGGA	795	0.0	38.773586	4
ACGTTAG	35	6.2439103E-6	38.571426	2
ATAACGC	35	6.2439103E-6	38.571426	11
CGTTTAG	35	6.2439103E-6	38.571426	2
>>END_MODULE