FastQCFastQC Report
Thu 26 May 2016
SRR1545760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1545760_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences287767
Sequences flagged as poor quality0
Sequence length50
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9310.32352563011047136No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4420.15359648604600248No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4040.1403913582863914No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3930.13656882130334613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTAG351.4410216E-744.02
TTAGGCC502.7284841E-1144.05
AGGTAGC254.4344983E-544.07
ATAGGCG254.4344983E-544.05
GCGAATG254.4344983E-544.01
ACCATAC207.846634E-444.044
TCCGAAC207.846634E-444.030
TAGGTGT207.846634E-444.06
TACGGGA502.7284841E-1144.04
ACGGGAT254.4344983E-544.05
CGTTAAG207.846634E-444.02
TCGTGGC207.846634E-444.038
TGCGCAA207.846634E-444.01
CTACGGG502.7284841E-1144.03
TAAGGCG551.8189894E-1244.05
CGGTCTA302.5210793E-644.031
CGCAATA207.846634E-444.037
CTAGGAT408.274583E-944.05
AGTACGG207.846634E-444.02
CGTTTTA6600.043.6666641
Produced by FastQC (version 0.11.5)