##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1545760_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	287767
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.560105919024767	31.0	31.0	33.0	30.0	34.0
2	31.76291930624429	31.0	31.0	34.0	30.0	34.0
3	31.824166773813538	31.0	31.0	34.0	30.0	34.0
4	35.71912345751945	37.0	35.0	37.0	35.0	37.0
5	27.22653396671613	35.0	26.0	37.0	0.0	37.0
6	31.4139633800957	36.0	28.0	37.0	17.0	37.0
7	14.811899905131582	0.0	0.0	32.0	0.0	37.0
8	24.99543380582207	17.0	17.0	35.0	17.0	37.0
9	33.166002356072795	32.0	32.0	37.0	28.0	39.0
10	36.0805964547707	37.0	35.0	39.0	32.0	39.0
11	37.07653414046781	39.0	37.0	39.0	34.0	39.0
12	37.04205485688074	39.0	35.0	39.0	33.0	39.0
13	36.550052646759355	39.0	35.0	39.0	33.0	39.0
14	37.90024915991062	40.0	36.0	41.0	33.0	41.0
15	38.16869550712903	40.0	36.0	41.0	33.0	41.0
16	38.2277571785507	40.0	36.0	41.0	34.0	41.0
17	38.26365775088874	40.0	36.0	41.0	34.0	41.0
18	38.23473157102795	40.0	36.0	41.0	34.0	41.0
19	38.16992219399723	40.0	36.0	41.0	34.0	41.0
20	38.177563792929696	40.0	36.0	41.0	34.0	41.0
21	38.0435247961024	40.0	36.0	41.0	34.0	41.0
22	37.96032901618323	40.0	35.0	41.0	34.0	41.0
23	37.876942109414905	40.0	35.0	41.0	34.0	41.0
24	37.7234116490077	40.0	35.0	41.0	33.0	41.0
25	37.55343732950616	39.0	35.0	41.0	33.0	41.0
26	37.319372964933436	39.0	35.0	40.0	33.0	41.0
27	37.11406450357407	39.0	35.0	40.0	33.0	41.0
28	37.124718956655904	39.0	35.0	40.0	33.0	41.0
29	37.07242665072785	39.0	35.0	40.0	33.0	41.0
30	36.89621117084308	38.0	35.0	40.0	32.0	41.0
31	36.72279656805679	38.0	35.0	40.0	32.0	41.0
32	36.501732304260045	38.0	35.0	40.0	31.0	41.0
33	36.32015484749815	38.0	35.0	40.0	31.0	41.0
34	36.106506305448505	38.0	35.0	40.0	30.0	41.0
35	35.96935367849684	38.0	35.0	40.0	30.0	41.0
36	35.77689589146775	38.0	35.0	40.0	30.0	41.0
37	35.61899036373177	38.0	35.0	40.0	29.0	41.0
38	35.46527572654266	38.0	34.0	40.0	29.0	41.0
39	35.421636254330764	38.0	34.0	40.0	29.0	41.0
40	35.19572084359916	38.0	34.0	40.0	28.0	41.0
41	35.03863542379773	38.0	34.0	40.0	27.0	41.0
42	35.013406679709625	37.0	34.0	40.0	27.0	41.0
43	34.91411106902459	37.0	34.0	40.0	27.0	41.0
44	34.69204947057863	37.0	34.0	40.0	26.0	40.0
45	34.480023769229966	37.0	34.0	40.0	26.0	40.0
46	34.43442437805586	36.0	34.0	39.0	26.0	40.0
47	34.280139835352905	36.0	33.0	39.0	26.0	40.0
48	34.20934645042691	36.0	33.0	39.0	26.0	40.0
49	34.08289692702777	36.0	33.0	39.0	25.0	40.0
50	34.1572278961799	36.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	7.0
16	6.0
17	35.0
18	76.0
19	163.0
20	356.0
21	539.0
22	923.0
23	1396.0
24	2219.0
25	3290.0
26	4000.0
27	4562.0
28	4710.0
29	5307.0
30	6667.0
31	9059.0
32	12788.0
33	24286.0
34	24886.0
35	30220.0
36	39514.0
37	72818.0
38	32133.0
39	7804.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.04409470161624	23.97286693748762	28.09738434219351	14.885654018702631
2	33.340515069483295	23.725097040313862	29.63751924299868	13.296868647204164
3	30.324880893222645	24.31550525251332	32.2291993175034	13.130414536760643
4	27.890272338384875	26.552384394319017	30.63311637540093	14.924226891895179
5	17.928740960568792	47.293470064322875	23.107931069233096	11.669857905875238
6	20.782786073455263	41.09088255428871	28.013983535290706	10.112347836965323
7	38.726469678594135	56.43802103785354	3.8555498024443384	0.9799594811079797
8	87.59517248329377	2.875590321336359	7.656194073677663	1.8730431216922023
9	83.02307074820949	5.0023108973579316	9.15011102732419	2.82450732710839
10	50.67224525397284	22.405626774439042	14.381774143664842	12.540353827923283
11	44.774418192496015	19.902907560630652	20.07075168452255	15.251922562350792
12	39.26892242682448	20.848464208891222	25.708646231152287	14.173967133132011
13	21.96360249785417	37.15783950209718	25.98873394100088	14.88982405904777
14	16.852175544798396	39.66472875625072	28.806986207591557	14.676109491359329
15	15.898626319209638	22.304503296069388	45.95801464379168	15.838855740929295
16	17.185778772409623	17.56073490011016	45.02114557958348	20.232340747896735
17	18.077472399545467	17.456831394843743	30.098308701136684	34.36738750447411
18	22.317360920466907	22.786490459295194	33.51739428078967	21.378754339448232
19	29.48983031410829	22.585633516004265	26.41164553267053	21.512890637216913
20	30.43469195564467	22.50501273599822	25.39207066828372	21.66822464007339
21	23.51451000288428	26.460991010088026	28.709337762842857	21.315161224184845
22	23.34180083192305	23.775485027817645	25.882050408837703	27.000663731421604
23	21.116736804428584	28.911584719582162	25.58667255105693	24.385005924932322
24	21.182414939864543	23.648298797290863	37.065751111142	18.103535151702594
25	20.094729416507104	23.25596750148558	34.34445228257583	22.304850799431485
26	18.23176389231566	31.85667571333753	27.591071943621053	22.32048845072576
27	19.983875843998792	29.973902497506664	29.9853701084558	20.056851550038747
28	17.8932956176351	26.422765640257566	37.46259995065452	18.22133879145281
29	18.179985891363497	25.043872299464496	35.622222144999256	21.15391966417275
30	20.874874464410443	28.198855323925258	31.93138893618796	18.99488127547634
31	30.085798580101265	25.023369601100892	24.60601806322476	20.284813755573087
32	29.950619772246295	25.451493743201965	26.448133385690507	18.149753098861233
33	28.21831551220258	25.170711026629185	25.347242734573456	21.26373072659478
34	20.465863007224595	26.42485066043014	28.820538838713265	24.288747493632002
35	21.853443932070043	24.78706731487627	30.30472569822113	23.054763054832556
36	30.832930808605575	24.80861252332616	25.324307512675187	19.03414915539308
37	21.838501287499955	31.55504279503904	26.70945591398597	19.897000003475036
38	22.50848776961917	32.240666928452534	22.902209078872836	22.348636223055458
39	22.132836635194444	29.352218982718657	25.860505200387813	22.654439181699082
40	24.228629411989562	24.71235409202584	26.072134747903686	24.986881748080915
41	19.323271952656143	23.865140895238163	27.067036873581753	29.744550278523946
42	22.471304909875002	27.12298491487905	23.37168612106322	27.034024054182726
43	23.18299179544562	25.872320314699042	25.464003864237387	25.48068402561795
44	21.063221286665947	29.29627094142136	27.77802875242818	21.862479019484514
45	19.881709855542855	33.894087925300674	23.78139258497326	22.44280963418321
46	22.898386541889792	30.153214232347697	26.537094246386832	20.411304979375675
47	23.043642947245516	26.67088304079342	27.537208922496326	22.748265089464738
48	23.83386559264961	24.16573130345036	29.56245851678615	22.437944587113883
49	21.43504988410763	24.230366928800038	31.33993821390222	22.994644973190116
50	20.50895342412438	30.51322771547815	27.43504293404039	21.542775926357088
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	140.0
1	259.0
2	378.0
3	1699.5
4	3021.0
5	2273.0
6	1525.0
7	1530.0
8	1535.0
9	1583.0
10	1631.0
11	1634.0
12	1637.0
13	1561.5
14	1486.0
15	1344.5
16	1203.0
17	1167.0
18	1131.0
19	1128.5
20	1126.0
21	1152.5
22	1179.0
23	1297.0
24	1415.0
25	1659.0
26	1903.0
27	2291.5
28	2680.0
29	3117.0
30	3554.0
31	4098.5
32	4643.0
33	5054.5
34	5466.0
35	5878.5
36	6291.0
37	7788.5
38	9286.0
39	13060.0
40	16834.0
41	20726.5
42	24619.0
43	25650.5
44	26682.0
45	26209.5
46	25737.0
47	24788.5
48	23840.0
49	23014.0
50	22188.0
51	20841.0
52	19494.0
53	17546.0
54	15598.0
55	14580.0
56	13562.0
57	12715.5
58	11869.0
59	10928.5
60	9988.0
61	9092.0
62	8196.0
63	7082.0
64	5968.0
65	4969.5
66	3971.0
67	3455.0
68	2939.0
69	2535.5
70	2132.0
71	1734.0
72	1336.0
73	1071.0
74	806.0
75	593.0
76	380.0
77	305.5
78	231.0
79	155.5
80	80.0
81	63.5
82	47.0
83	34.5
84	22.0
85	15.0
86	8.0
87	8.0
88	8.0
89	4.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	287767.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.0043202031122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.39391156689567	21.840564592122817
2	10.11598566225465	7.082064025833075
3	6.246130136895712	6.559246180266196
4	4.288996604832781	6.005336420225111
5	3.338995326268922	5.843963077870621
6	2.4989964593197236	5.248540334908274
7	2.017997139849122	4.944703263657027
8	1.5919977436057429	4.458143902384603
9	1.232998252427849	3.884423907407597
>10	6.234991162925834	32.395752345927214
>50	0.02999995747999599	0.7802451914581813
>100	0.007999988661332265	0.6311269987434279
>500	9.99998582666533E-4	0.3258897591958577
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	931	0.32352563011047136	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	442	0.15359648604600248	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	404	0.1403913582863914	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	393	0.13656882130334613	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0034750336209502827	0.0
2	0.0	0.0	0.0	0.026757758881317175	0.0
3	0.0	0.0	0.0	0.03579284629578791	0.0
4	0.0	0.0	0.0	0.05004048414168407	0.0
5	0.0	0.0	0.0	0.08340080690280678	0.0
6	0.0	0.0	0.0	0.14525640535572182	0.0
7	0.0	0.0	0.0	0.1893893323417904	0.0
8	0.0	0.0	0.0	0.43229418244621515	0.0
9	0.0	0.0	0.0	0.5421052448682441	0.0
10	0.0	0.0	0.0	0.7231544965197538	0.0
11	0.0	0.0	0.0	0.8826585397213718	0.0
12	0.0	0.0	0.0	1.0220073879214782	0.0
13	0.0	0.0	0.0	1.0793454426671578	0.0
14	0.0	0.0	0.0	1.1005431477549545	0.0
15	0.0	0.0	0.0	1.1384210142233127	0.0
16	0.0	0.0	0.0	1.2134817404358387	0.0
17	0.0	0.0	0.0	1.2982725607870256	0.0
18	0.0	0.0	0.0	1.4365788989008468	0.0
19	0.0	0.0	0.0	1.4970444839053818	0.0
20	0.0	0.0	0.0	1.5627226193413422	0.0
21	0.0	0.0	0.0	1.6367408354675832	0.0
22	0.0	0.0	0.0	1.719794139008295	0.0
23	0.0	0.0	0.0	1.8031949459111016	0.0
24	0.0	0.0	0.0	1.8671355645365868	0.0
25	0.0	0.0	0.0	1.9248211226443617	0.0
26	0.0	0.0	0.0	1.9745141034239506	0.0
27	0.0	0.0	0.0	2.02351207747935	0.0
28	0.0	0.0	0.0	2.0728575548968435	0.0
29	0.0	0.0	0.0	2.1347131533497588	0.0
30	0.0	0.0	0.0	2.2108163896485697	0.0
31	0.0	0.0	0.0	2.295954713361852	0.0
32	0.0	0.0	0.0	2.3661503925050473	0.0
33	0.0	0.0	0.0	2.433913548113578	0.0
34	0.0	0.0	0.0	2.5044567306188688	0.0
35	0.0	0.0	0.0	2.606622719074807	0.0
36	0.0	0.0	0.0	2.6809884385631433	0.0
37	0.0	0.0	0.0	2.764736748828045	0.0
38	0.0	0.0	0.0	2.8397974750405712	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATTTAG	35	1.4410216E-7	44.0	2
TTAGGCC	50	2.7284841E-11	44.0	5
AGGTAGC	25	4.4344983E-5	44.0	7
ATAGGCG	25	4.4344983E-5	44.0	5
GCGAATG	25	4.4344983E-5	44.0	1
ACCATAC	20	7.846634E-4	44.0	44
TCCGAAC	20	7.846634E-4	44.0	30
TAGGTGT	20	7.846634E-4	44.0	6
TACGGGA	50	2.7284841E-11	44.0	4
ACGGGAT	25	4.4344983E-5	44.0	5
CGTTAAG	20	7.846634E-4	44.0	2
TCGTGGC	20	7.846634E-4	44.0	38
TGCGCAA	20	7.846634E-4	44.0	1
CTACGGG	50	2.7284841E-11	44.0	3
TAAGGCG	55	1.8189894E-12	44.0	5
CGGTCTA	30	2.5210793E-6	44.0	31
CGCAATA	20	7.846634E-4	44.0	37
CTAGGAT	40	8.274583E-9	44.0	5
AGTACGG	20	7.846634E-4	44.0	2
CGTTTTA	660	0.0	43.666664	1
>>END_MODULE